k 2025

Seeing is Believing: 3D Mapping of Tick-Borne Encephalitis Virus Distribution in Mouse Brain Using TurboGFP Reporter Virus

BERÁNKOVÁ, Michaela; Simone LEONI; Jan HAVIERNIK; Jiří HOLOUBEK; Daniel RŮŽEK et al.

Základní údaje

Originální název

Seeing is Believing: 3D Mapping of Tick-Borne Encephalitis Virus Distribution in Mouse Brain Using TurboGFP Reporter Virus

Autoři

BERÁNKOVÁ, Michaela; Simone LEONI; Jan HAVIERNIK; Jiří HOLOUBEK a Daniel RŮŽEK

Vydání

NIVB Meeting 2025, 2025

Další údaje

Typ výsledku

Prezentace na konferencích

Odkazy

Označené pro přenos do RIV

Ne

Klíčová slova česky

virus klíšťové encefalitidy; light-sheet mikroskopie; fluorescenční mikroskopie

Klíčová slova anglicky

tick-borne encephalitis virus; light-sheet microscopy; fluorescent microscopy

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 18. 11. 2025 17:18, Mgr. Michaela Beránková

Anotace

V originále

Tick-borne encephalitis virus (TBEV) is a neurotropic orthoflavivirus that invades the central nervous system, causing severe neurological disease and posing a significant public health threat. Traditionally, detecting TBEV in infected cells or animal models relied on labor-intensive secondary approaches, preventing real-time observation of infection and limiting studies of viral spread and host responses. We developed a stable recombinant TBEV expressing TurboGFP, allowing direct visualization of infection in cells, tissue slices, and mice. The reporter virus enables tracking of viral replication, spread, and tropism, including whole brain imaging in 3D using light-sheet microscopy. This tool could also be used for antiviral testing and provides a versatile platform for studying TBEV biology and pathogenesis.