Multielectrode array technique as an effective tool for
studying cardiac electrophysiology and its changes in
pathology: problematic aspects of analysis

k 2025

Multielectrode array technique as an effective tool for studying cardiac electrophysiology and its changes in pathology: problematic aspects of analysis

ŠVECOVÁ, Olga; Martin KRÁL; Pavel JURÁK; Josef HALÁMEK; Milena ŠIMURDOVÁ et al.

Základní údaje

Originální název

Multielectrode array technique as an effective tool for studying cardiac electrophysiology and its changes in pathology: problematic aspects of analysis

Autoři

ŠVECOVÁ, Olga ; Martin KRÁL ; Pavel JURÁK; Josef HALÁMEK; Milena ŠIMURDOVÁ; Jiří ŠIMURDA a Markéta BÉBAROVÁ

Vydání

Czech Cardiovascular Research and Innovation Days 2025 + 11th European Section Meeting of the International Academy of Cardiovascular Sciences, 2025

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

30201 Cardiac and Cardiovascular systems

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/25:00142673

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

microelectrode array; field potential duration; Bazett´s formula; Fridericia´s formula; filtering

Příznaky

Mezinárodní význam

Změněno: 24. 11. 2025 11:24, doc. MUDr. Markéta Bébarová, Ph.D.

Anotace

V originále

The microelectrode array (MEA) is an easy, high-throughput method, ideal for obtaining a large amount of data from excitable cells, including cardiomyocytes. In connection with cardiomyocytes derived from human-induced pluripotent stem cells, it represents a valuable tool for translational research and personalized medicine, both related to cardiac pathology and drug effects. In this study, we focused on problematic aspects of MEA recording and analysis. By examining more than 120 original articles using MEA and any cardiac preparation, we detected an inconsistency in the acquisition setting. It is striking that only one-third of the studies provided complete information about the filtering of the signal. We investigated how the filtering may alter the MEA signal and the evaluation of the field potential duration (FPD), a parameter analogous to the QT interval on ECG. Based on the analysis, we recommend using the high-pass filter as low as possible, because even the frequently used 1-Hz filter may substantially distort the data and FPD value. We also studied the impact of the correction of FPD on the cycle length (CL), resulting in FPDc values, for which the standard correction formulas used in clinical practice, namely Bazett´s and Fridericia´s formulas, are usually used in MEA studies. The data demonstrated that such correction without the proof of proportionality between FPD and CL may considerably alter the resulting FPDc values and should be avoided. The performed analysis emphasizes a thorough inspection of both the ‘raw’ and filtered signals to estimate proper FPD values, as well as a careful determination of the relationship between FPD and CL before using any correction formula.

Návaznosti

MUNI/A/1641/2024, interní kód MU

Název: Od buňky k pacientovi II

Investor: Masarykova univerzita, Od buňky k pacientovi II

NU22-02-00348, projekt VaV

Název: Funkční hodnocení genetických variant u případů klinicky „skutečné“ idiopatické fibrilace komor: in vitro a in silico modelování s cílem odhalit arytmogenní mechanismus

Investor: Ministerstvo zdravotnictví ČR, Funkční hodnocení genetických variant u případů klinicky „skutečné“ idiopatické fibrilace komor: in vitro a in silico modelování s cílem odhalit arytmogenní mechanismus, Podprogram 1 - standardní

Citovat

ŠVECOVÁ, Olga; Martin KRÁL; Pavel JURÁK; Josef HALÁMEK; Milena ŠIMURDOVÁ; Jiří ŠIMURDA a Markéta BÉBAROVÁ. Multielectrode array technique as an effective tool for studying cardiac electrophysiology and its changes in pathology: problematic aspects of analysis. In Czech Cardiovascular Research and Innovation Days 2025 + 11th European Section Meeting of the International Academy of Cardiovascular Sciences. 2025.

@proceedings{2535198,
   author = {Švecová, Olga and Král, Martin and Jurák, Pavel and Halámek, Josef and Šimurdová, Milena and Šimurda, Jiří and Bébarová, Markéta},
   booktitle = {Czech Cardiovascular Research and Innovation Days 2025 + 11th European Section Meeting of the International Academy of Cardiovascular Sciences},
   keywords = {microelectrode array; field potential duration; Bazett´s formula; Fridericia´s formula; filtering},
   language = {eng},
   title = {Multielectrode array technique as an effective tool for studying cardiac electrophysiology and its changes in pathology: problematic aspects of analysis},
   year = {2025}
}

TY  - CONF
ID  - 2535198
AU  - Švecová, Olga - Král, Martin - Jurák, Pavel - Halámek, Josef - Šimurdová, Milena - Šimurda, Jiří - Bébarová, Markéta
PY  - 2025
TI  - Multielectrode array technique as an effective tool for studying cardiac electrophysiology and its changes in pathology: problematic aspects of analysis
KW  - microelectrode array
KW  - field potential duration
KW  - Bazett´s formula
KW  - Fridericia´s formula
KW  - filtering
N2  - The microelectrode array (MEA) is an easy, high-throughput method, ideal for obtaining a large amount of data from excitable cells, including cardiomyocytes. In connection with cardiomyocytes derived from human-induced pluripotent stem cells, it represents a valuable tool for translational research and personalized medicine, both related to cardiac pathology and drug effects. In this study, we focused on problematic aspects of MEA recording and analysis. By examining more than 120 original articles using MEA and any cardiac preparation, we detected an inconsistency in the acquisition setting. It is striking that only one-third of the studies provided complete information about the filtering of the signal. We investigated how the filtering may alter the MEA signal and the evaluation of the field potential duration (FPD), a parameter analogous to the QT interval on ECG. Based on the analysis, we recommend using the high-pass filter as low as possible, because even the frequently used 1-Hz filter may substantially distort the data and FPD value. We also studied the impact of the correction of FPD on the cycle length (CL), resulting in FPDc values, for which the standard correction formulas used in clinical practice, namely Bazett´s and Fridericia´s formulas, are usually used in MEA studies. The data demonstrated that such correction without the proof of proportionality between FPD and CL may considerably alter the resulting FPDc values and should be avoided. The performed analysis emphasizes a thorough inspection of both the ‘raw’ and filtered signals to estimate proper FPD values, as well as a careful determination of the relationship between FPD and CL before using any correction formula.
ER  -

ŠVECOVÁ, Olga; Martin KRÁL; Pavel JURÁK; Josef HALÁMEK; Milena ŠIMURDOVÁ; Jiří ŠIMURDA a Markéta BÉBAROVÁ. Multielectrode array technique as an effective tool for studying cardiac electrophysiology and its changes in pathology: problematic aspects of analysis. In \textit{Czech Cardiovascular Research and Innovation Days 2025 + 11th European Section Meeting of the International Academy of Cardiovascular Sciences}. 2025.

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