A nuclear TRiC/CCT chaperonin assembles meiotic HORMAD proteins
into chromosome axes competent for crossing over

J 2025

A nuclear TRiC/CCT chaperonin assembles meiotic HORMAD proteins into chromosome axes competent for crossing over

ZETKA, Monique; Amirhossein PEZESHKI; Ryan DAWSON; J. HUIS IN'T VELD PIM; Steven J M JONES et al.

Základní údaje

Originální název

A nuclear TRiC/CCT chaperonin assembles meiotic HORMAD proteins into chromosome axes competent for crossing over

Autoři

ZETKA, Monique; Amirhossein PEZESHKI; Ryan DAWSON; J. HUIS IN'T VELD PIM; Steven J M JONES a Nicola SILVA

Vydání

Nature communications, BERLIN, NATURE PORTFOLIO, 2025, 2041-1723

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 15.700 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

ETS1; DNA-PKcs; chemoresistance; triple-negative breast cancer; transcriptional regulation

Štítky

14110513, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 12. 2025 13:28, Mgr. Tereza Miškechová

Anotace

V originále

The meiotic chromosome axis organizes chromatin and sets the stage for homolog pairing and recombination. Meiotic HORMA domain proteins (mHORMADs) are conserved axis components that conformationally transform during target binding. In C. elegans, four functionally distinct mHORMADs directly interact, but how binding between them is restricted to axis assembly is unknown. Using a mutation in the mHORMADs that delays axis assembly, we isolated a suppressor mutation in a TRiC (Tailless complex peptide 1 Ring Complex) chaperonin subunit that restored mHORMAD localization. CCT-4 associates with meiotic chromatin and forms in vivo complexes with mHORMADs, while germline disruption of TRiC results in axis defects, indicating a nuclear function for TRiC alongside meiotic chromosomes. We propose that chromosome-associated TRiC locally folds mHORMADs into the binding-competent conformation required for axis morphogenesis. More broadly, our results support the model that spatially-restricted folding by TRiC/CCT is a mechanism of controlling the assembly of multimeric complexes that function in tightly co-ordinated events.

Návaznosti

GA23-04918S, projekt VaV

Název: Mechanismus založený na modifikaci chromatinu osvětluje novou dráhu pro vytvoření meiotické synapse chromozomů

Investor: Grantová agentura ČR, Mechanismus založený na modifikaci chromatinu osvětluje novou dráhu pro vytvoření meiotické synapse chromozomů

Citovat

ZETKA, Monique; Amirhossein PEZESHKI; Ryan DAWSON; J. HUIS IN'T VELD PIM; Steven J M JONES a Nicola SILVA. A nuclear TRiC/CCT chaperonin assembles meiotic HORMAD proteins into chromosome axes competent for crossing over. Nature communications. BERLIN: NATURE PORTFOLIO, 2025, roč. 16, č. 1, s. 1-22. ISSN 2041-1723. Dostupné z: https://doi.org/10.1038/s41467-025-64403-0.

@article{2537914,
   author = {Zetka, Monique and Pezeshki, Amirhossein and Dawson, Ryan and Huis In't Veld Pim, J. and Jones, Steven J M and Silva, Nicola},
   article_location = {BERLIN},
   article_number = {1},
   doi = {https://doi.org/10.1038/s41467-025-64403-0},
   keywords = {ETS1; DNA-PKcs; chemoresistance; triple-negative breast cancer; transcriptional regulation},
   language = {eng},
   issn = {2041-1723},
   journal = {Nature communications},
   title = {A nuclear TRiC/CCT chaperonin assembles meiotic HORMAD proteins into chromosome axes competent for crossing over},
   url = {https://www.nature.com/articles/s41467-025-64403-0},
   volume = {16},
   year = {2025}
}

TY  - JOUR
ID  - 2537914
AU  - Zetka, Monique - Pezeshki, Amirhossein - Dawson, Ryan - Huis In't Veld Pim, J. - Jones, Steven J M - Silva, Nicola
PY  - 2025
TI  - A nuclear TRiC/CCT chaperonin assembles meiotic HORMAD proteins into chromosome axes competent for crossing over
JF  - Nature communications
VL  - 16
IS  - 1
SP  - 1-22
EP  - 1-22
PB  - NATURE PORTFOLIO
SN  - 20411723
KW  - ETS1
KW  - DNA-PKcs
KW  - chemoresistance
KW  - triple-negative breast cancer
KW  - transcriptional regulation
UR  - https://www.nature.com/articles/s41467-025-64403-0
N2  - The meiotic chromosome axis organizes chromatin and sets the stage for homolog pairing and recombination. Meiotic HORMA domain proteins (mHORMADs) are conserved axis components that conformationally transform during target binding. In C. elegans, four functionally distinct mHORMADs directly interact, but how binding between them is restricted to axis assembly is unknown. Using a mutation in the mHORMADs that delays axis assembly, we isolated a suppressor mutation in a TRiC (Tailless complex peptide 1 Ring Complex) chaperonin subunit that restored mHORMAD localization. CCT-4 associates with meiotic chromatin and forms in vivo complexes with mHORMADs, while germline disruption of TRiC results in axis defects, indicating a nuclear function for TRiC alongside meiotic chromosomes. We propose that chromosome-associated TRiC locally folds mHORMADs into the binding-competent conformation required for axis morphogenesis. More broadly, our results support the model that spatially-restricted folding by TRiC/CCT is a mechanism of controlling the assembly of multimeric complexes that function in tightly co-ordinated events.
ER  -

ZETKA, Monique; Amirhossein PEZESHKI; Ryan DAWSON; J. HUIS IN'T VELD PIM; Steven J M JONES a Nicola SILVA. A nuclear TRiC/CCT chaperonin assembles meiotic HORMAD proteins into chromosome axes competent for crossing over. \textit{Nature communications}. BERLIN: NATURE PORTFOLIO, 2025, roč.~16, č.~1, s.~1-22. ISSN~2041-1723. Dostupné z: https://doi.org/10.1038/s41467-025-64403-0.

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