The quest for metabolic biomarkers of IVF outcomes: a
meta-analysis and critical review

J 2025

The quest for metabolic biomarkers of IVF outcomes: a meta-analysis and critical review

ŠMAK, Pavel; Jan JUŘICA; Jana GREGOROVÁ; Volodymyr POROKH; Ondřej PEŠ et al.

Základní údaje

Originální název

The quest for metabolic biomarkers of IVF outcomes: a meta-analysis and critical review

Autoři

ŠMAK, Pavel; Jan JUŘICA; Jana GREGOROVÁ ; Volodymyr POROKH; Ondřej PEŠ a Zuzana HOLUBCOVÁ

Vydání

Frontiers in Cell and Developmental Biology, FRONTIERS MEDIA SA, LAUSANNE, 2025, 2296-634X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

20602 Medical laboratory technology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.300 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

spent culture medium; metabolomics; embryo selection; biomarkers; in vitro fertilization; assisted reproduction

Štítky

14110512, 14110516, 14110517, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 12. 2025 14:24, Mgr. Tereza Miškechová

Anotace

V originále

Spent culture media (SCM) analysis offers a promising, non-invasive strategy for assessing embryo viability and implantation potential in in vitro fertilization (IVF). By profiling the consumption and secretion of low molecular weight metabolites, SCM analysis may offer valuable insights into embryonic metabolic activity and developmental competence. Identifying reliable biomarkers in SCM has the potential to support more objective embryo selection and reduce time to pregnancy. This Bayesian meta-analysis synthesizes quantitative evidence from studies reporting metabolite concentrations in SCM in relation to IVF outcomes. From a comprehensive literature search identifying 175 studies, 10 met strict inclusion criteria, providing concentration-based data suitable for standardized effect size estimation. Using a multilevel modeling approach, we integrated data across heterogeneous study designs and found seven metabolites positively and ten negatively associated with favorable IVF outcomes. To complement this quantitative synthesis, we critically appraised 14 additional studies excluded from the meta-analysis due to missing calibration data or insufficient methodological transparency. This dual approach highlights recurring methodological challenges and underscores the need for standardized protocols, validated analytical methods, and transparent reporting in SCM research. Overall, the findings illustrate both the potential and the current limitations of SCM metabolic profiling. We provide practical recommendations for improving study design and reproducibility, with the goal of advancing SCM analysis toward clinically relevant biomarker validation.

Návaznosti

MUNI/A/1676/2024, interní kód MU

Název: Využití chemických a bioanalytických technik pro zkoumání biochemické podstaty vybraných patologických procesů.

Investor: Masarykova univerzita, Využití chemických a bioanalytických technik pro zkoumání biochemické podstaty vybraných patologických procesů.

MUNI/A/1722/2024, interní kód MU

Název: Specifický farmakologický výzkum v oblasti farmakokinetiky, behaviorální neuropsychofarmakologie a personalizované farmakoterapie v onkologii

Investor: Masarykova univerzita, Specifický farmakologický výzkum v oblasti farmakokinetiky, behaviorální neuropsychofarmakologie a personalizované farmakoterapie v onkologii

MUNI/A/1738/2024, interní kód MU

Název: Zdroje pro tkáňové inženýrství 15

Investor: Masarykova univerzita, Zdroje pro tkáňové inženýrství 15

NU22-08-00543, projekt VaV

Název: Nové biomarkery pro neinvazivní in vitro diagnostiku v klinické embryologii (Akronym: Analyza IVF médií)

Investor: Ministerstvo zdravotnictví ČR, Nové biomarkery pro neinvazivní in vitro diagnostiku v klinické embryologii, Podprogram 1 - standardní

Citovat

ŠMAK, Pavel; Jan JUŘICA; Jana GREGOROVÁ; Volodymyr POROKH; Ondřej PEŠ a Zuzana HOLUBCOVÁ. The quest for metabolic biomarkers of IVF outcomes: a meta-analysis and critical review. Frontiers in Cell and Developmental Biology. FRONTIERS MEDIA SA: LAUSANNE, 2025, roč. 13, October 2025, s. 1-12. ISSN 2296-634X. Dostupné z: https://doi.org/10.3389/fcell.2025.1640807.

@article{2537939,
   author = {Šmak, Pavel and Juřica, Jan and Gregorová, Jana and Porokh, Volodymyr and Peš, Ondřej and Holubcová, Zuzana},
   article_location = {FRONTIERS MEDIA SA},
   article_number = {October 2025},
   doi = {https://doi.org/10.3389/fcell.2025.1640807},
   keywords = {spent culture medium; metabolomics; embryo selection; biomarkers; in vitro fertilization; assisted reproduction},
   language = {eng},
   issn = {2296-634X},
   journal = {Frontiers in Cell and Developmental Biology},
   title = {The quest for metabolic biomarkers of IVF outcomes: a meta-analysis and critical review},
   url = {https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2025.1640807/full},
   volume = {13},
   year = {2025}
}

TY  - JOUR
ID  - 2537939
AU  - Šmak, Pavel - Juřica, Jan - Gregorová, Jana - Porokh, Volodymyr - Peš, Ondřej - Holubcová, Zuzana
PY  - 2025
TI  - The quest for metabolic biomarkers of IVF outcomes: a meta-analysis and critical review
JF  - Frontiers in Cell and Developmental Biology
VL  - 13
IS  - October 2025
SP  - 1-12
EP  - 1-12
PB  - LAUSANNE
SN  - 2296634X
KW  - spent culture medium
KW  - metabolomics
KW  - embryo selection
KW  - biomarkers
KW  - in vitro fertilization
KW  - assisted reproduction
UR  - https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2025.1640807/full
N2  - Spent culture media (SCM) analysis offers a promising, non-invasive strategy for assessing embryo viability and implantation potential in in vitro fertilization (IVF). By profiling the consumption and secretion of low molecular weight metabolites, SCM analysis may offer valuable insights into embryonic metabolic activity and developmental competence. Identifying reliable biomarkers in SCM has the potential to support more objective embryo selection and reduce time to pregnancy. This Bayesian meta-analysis synthesizes quantitative evidence from studies reporting metabolite concentrations in SCM in relation to IVF outcomes. From a comprehensive literature search identifying 175 studies, 10 met strict inclusion criteria, providing concentration-based data suitable for standardized effect size estimation. Using a multilevel modeling approach, we integrated data across heterogeneous study designs and found seven metabolites positively and ten negatively associated with favorable IVF outcomes. To complement this quantitative synthesis, we critically appraised 14 additional studies excluded from the meta-analysis due to missing calibration data or insufficient methodological transparency. This dual approach highlights recurring methodological challenges and underscores the need for standardized protocols, validated analytical methods, and transparent reporting in SCM research. Overall, the findings illustrate both the potential and the current limitations of SCM metabolic profiling. We provide practical recommendations for improving study design and reproducibility, with the goal of advancing SCM analysis toward clinically relevant biomarker validation.
ER  -

ŠMAK, Pavel; Jan JUŘICA; Jana GREGOROVÁ; Volodymyr POROKH; Ondřej PEŠ a Zuzana HOLUBCOVÁ. The quest for metabolic biomarkers of IVF outcomes: a meta-analysis and critical review. \textit{Frontiers in Cell and Developmental Biology}. FRONTIERS MEDIA SA: LAUSANNE, 2025, roč.~13, October 2025, s.~1-12. ISSN~2296-634X. Dostupné z: https://doi.org/10.3389/fcell.2025.1640807.

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