Fully Immunocompetent Porcine Model of Complicated Skin and
Soft Tissue Infection Caused by Carbapenem-Resistant
Pseudomonas aeruginosa

a 2025

Fully Immunocompetent Porcine Model of Complicated Skin and Soft Tissue Infection Caused by Carbapenem-Resistant Pseudomonas aeruginosa

VACEK, Lukáš; Břetislav LIPOVÝ; Dominika POLAŠTÍK KLEKNEROVÁ; Edita JEKLOVÁ; Lenka LIŠKOVÁ et al.

Základní údaje

Originální název

Fully Immunocompetent Porcine Model of Complicated Skin and Soft Tissue Infection Caused by Carbapenem-Resistant Pseudomonas aeruginosa

Autoři

VACEK, Lukáš ; Břetislav LIPOVÝ; Dominika POLAŠTÍK KLEKNEROVÁ; Edita JEKLOVÁ; Lenka LIŠKOVÁ; Jakub HOLOUBEK; Dominika MATYSKOVÁ a Filip RŮŽIČKA

Vydání

FEMS MICRO Milan 2025, 2025

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10606 Microbiology

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/25:00144756

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

porcine model; skin infection; Pseudomonas; carbapenem

Příznaky

Mezinárodní význam

Změněno: 16. 12. 2025 14:03, Mgr. Lukáš Vacek, Ph.D.

Anotace

V originále

Carbapenem-resistant Pseudomonas aeruginosa (CRPA) poses a significant threat to patients, often resulting in life-threatening infections. With increasing antimicrobial resistance, novel therapeutic strategies are urgently needed. Although animal models are crucial for preclinical studies, limited data are available for porcine models, more specifically for CRPA complicated skin and soft tissue infections (cSSTI). The study presents a novel porcine model inducing and sustaining cSSTI caused by CRPA. Six fully immunocompetent pigs (120 wounds) were used to develop wound infection and evaluate the progression of cSSTI in the model. Full-thickness skin defects (5 × 5 cm) with fascial incisions were surgically performed to introduce cSSTI. CRPA clinical strain FF2 (producing VIM carbapenemase) was used for wound infection development. Microbiological and histopathological assessment was performed by processing the tissue biopsy samples. The model demonstrated bacterial loads of 107 CFU/gram of tissue and higher with extensive areas of bacterial cells within the wound tissue and on its surface. The cSSTI fully developed within three days and remained as such for 14 days post-infection. The histopathological evaluation confirmed the full development of cSSTI. The samples were characterized by significant tissue infiltration by inflammatory cells, with a predominance of polymorphonuclear cells and macrophages. This fully immunocompetent model of cSSTI caused by CRPA will serve in the development of novel therapeutical strategies (including antimicrobial peptides and enzymes, phage therapy, etc.) and corresponding preclinical studies. All immunological processes associated with the wound healing process (especially the inflammatory and proliferative phases) are preserved.

Návaznosti

NU22-05-00475, projekt VaV

Název: Léčba infekcí způsobených multirezistentními kmeny bakterií pomocí nových antibakteriálních přípravků založených na katelicidinových antimikrobiálních peptidech

Investor: Ministerstvo zdravotnictví ČR, Léčba infekcí způsobených multirezistentními kmeny bakterií pomocí nových antibakteriálních přípravků založených na katelicidinových antimikrobiálních peptidech, Podprogram 1 - standardní

Citovat

VACEK, Lukáš; Břetislav LIPOVÝ; Dominika POLAŠTÍK KLEKNEROVÁ; Edita JEKLOVÁ; Lenka LIŠKOVÁ; Jakub HOLOUBEK; Dominika MATYSKOVÁ a Filip RŮŽIČKA. Fully Immunocompetent Porcine Model of Complicated Skin and Soft Tissue Infection Caused by Carbapenem-Resistant Pseudomonas aeruginosa. In FEMS MICRO Milan 2025. 2025.

@proceedings{2540952,
   author = {Vacek, Lukáš and Lipový, Břetislav and Polaštík Kleknerová, Dominika and Jeklová, Edita and Lišková, Lenka and Holoubek, Jakub and Matysková, Dominika and Růžička, Filip},
   booktitle = {FEMS MICRO Milan 2025},
   keywords = {porcine model; skin infection; Pseudomonas; carbapenem},
   language = {eng},
   title = {Fully Immunocompetent Porcine Model of Complicated Skin and Soft Tissue Infection Caused by Carbapenem-Resistant Pseudomonas aeruginosa},
   url = {https://www.femsmicro.org/abstracts-overview},
   year = {2025}
}

TY  - CONF
ID  - 2540952
AU  - Vacek, Lukáš - Lipový, Břetislav - Polaštík Kleknerová, Dominika - Jeklová, Edita - Lišková, Lenka - Holoubek, Jakub - Matysková, Dominika - Růžička, Filip
PY  - 2025
TI  - Fully Immunocompetent Porcine Model of Complicated Skin and Soft Tissue Infection Caused by Carbapenem-Resistant Pseudomonas aeruginosa
KW  - porcine model
KW  - skin infection
KW  - Pseudomonas
KW  - carbapenem
UR  - https://www.femsmicro.org/abstracts-overview
N2  - Carbapenem-resistant Pseudomonas aeruginosa (CRPA) poses a significant threat to patients, often resulting in life-threatening infections. With increasing antimicrobial resistance, novel therapeutic strategies are urgently needed. Although animal models are crucial for preclinical studies, limited data are available for porcine models, more specifically for CRPA complicated skin and soft tissue infections (cSSTI). The study presents a novel porcine model inducing and sustaining cSSTI caused by CRPA. Six fully immunocompetent pigs (120 wounds) were used to develop wound infection and evaluate the progression of cSSTI in the model. Full-thickness skin defects (5 × 5 cm) with fascial incisions were surgically performed to introduce cSSTI. CRPA clinical strain FF2 (producing VIM carbapenemase) was used for wound infection development. Microbiological and histopathological assessment was performed by processing the tissue biopsy samples. The model demonstrated bacterial loads of 107 CFU/gram of tissue and higher with extensive areas of bacterial cells within the wound tissue and on its surface. The cSSTI fully developed within three days and remained as such for 14 days post-infection. The histopathological evaluation confirmed the full development of cSSTI. The samples were characterized by significant tissue infiltration by inflammatory cells, with a predominance of polymorphonuclear cells and macrophages. This fully immunocompetent model of cSSTI caused by CRPA will serve in the development of novel therapeutical strategies (including antimicrobial peptides and enzymes, phage therapy, etc.) and corresponding preclinical studies. All immunological processes associated with the wound healing process (especially the inflammatory and proliferative phases) are preserved.
ER  -

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