2025
CSF extracellular vesicle RNAs as biomarkers in gliomas
SMRČKA, Martin; Michaela RUČKOVÁ; František SIEGL; Marek VEČEŘA; Václav VYBÍHAL et al.Základní údaje
Originální název
CSF extracellular vesicle RNAs as biomarkers in gliomas
Autoři
SMRČKA, Martin; Michaela RUČKOVÁ; František SIEGL ORCID; Marek VEČEŘA; Václav VYBÍHAL; Martina HYZDALOVA; Jan KOTOUCEK; Nicol STRAKOVA; Pavel KULICH; Pavel FADRUS a Jiří ŠÁNA
Vydání
WFNS Dubai, 2025
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
30212 Surgery
Stát vydavatele
Spojené arabské emiráty
Utajení
není předmětem státního či obchodního tajemství
Označené pro přenos do RIV
Ano
Organizační jednotka
Lékařská fakulta
Klíčová slova anglicky
gliomas; CSF extracellular vesicle RNAs
Příznaky
Mezinárodní význam
Změněno: 17. 12. 2025 12:21, Mgr. Tereza Miškechová
Anotace
V originále
Background: Gliomas are aggressive brain tumors with poor prognosis, often developing resistance to therapies such as radiotherapy. Non-invasive biomarkers are urgently needed to improve diagnosis and monitoring, and microRNAs (miRNAs) in cerebrospinal fluid (CSF) extracellular vesicles (EVs) represent a promising source that may reflect tumor biology. Methods: EVs were isolated from CSF of 100 glioma patients (56 IDH-wildtype glioblastomas, 28 other high-grade, 16 low-grade). After evaluating three RNA extraction kits, the highest-yielding kit (Qiagen miRNeasy) was used for small RNA sequencing. Expression profiles of EV miRNAs were compared across glioma subtypes. Results: The Qiagen kit produced the highest EV RNA yield and library quality, enabling robust small RNA sequencing. EV miRNA profiling identified multiple abundant miRNAs with oncogenic (miR-21-5p), tumor-suppressive (miR-204-5p), or dual roles (miR-16-5p, let-7 family). EV miRNA profiles differed by tumor grade: high-grade tumors had elevated oncomiRs (e.g., miR-21-5p), whereas low-grade tumors showed higher levels of tumor-suppressive let-7 miRNAs. Some of these miRNAs are implicated in radioresistance. Conclusions: CSF EV miRNA profiling is a feasible, non-invasive approach for glioma biomarker discovery. The EV miRNA signatures distinguish glioma subtypes and may provide insight into molecular drivers of tumor progression and therapy resistance, including radioresistance. EV miRNAs could serve as novel biomarkers and therapeutic targets, informing neurosurgical patient stratification and personalized treatment.
Návaznosti
| NU22-03-00290, projekt VaV |
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| NW24-03-00221, projekt VaV |
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