Global Interactome Mapping Reveals Protumorigenic Interactions
of NF-κB in Breast Cancer

a 2025

Global Interactome Mapping Reveals Protumorigenic Interactions of NF-κB in Breast Cancer

LAPČÍK, Petr; R. Greg STACEY; David POTĚŠIL; Petr KULHÁNEK; Leonard J. FOSTER et al.

Základní údaje

Originální název

Global Interactome Mapping Reveals Protumorigenic Interactions of NF-κB in Breast Cancer

Autoři

LAPČÍK, Petr; R. Greg STACEY; David POTĚŠIL; Petr KULHÁNEK; Leonard J. FOSTER a Pavel BOUCHAL

Vydání

In Book of Abstracts of Czech Annual Cancer Research Meeting, Olomouc. 24.-26.11.2025, p. 41-42. 2025, 2025

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

Příznaky

Mezinárodní význam

Změněno: 5. 1. 2026 09:45, Mgr. Petr Lapčík, Ph.D.

Anotace

V originále

NF-κB pathway is involved in inflammation; however, recent data shows its role also in cancer development and progression, including metastasis. To understand the role of NF-κB interactome dynamics in cancer, in this work (1) we study the complexity of breast cancer interactome in luminal A breast cancer model and its rearrangement associated with NF-κB modulation. Liquid chromatography-mass spectrometry measurement of 160 size-exclusion chromatography fractions identifies 5460 protein groups. Seven thousand five hundred sixty eight interactions among these proteins have been reconstructed by PrInCE algorithm, of which 2564 have been validated in independent datasets. NF-κB modulation leads to rearrangement of protein complexes involved in NF-κB signaling and immune response, cell cycle regulation, and DNA replication. Central NF-κB transcription regulator RELA co-elutes with interactors of NF-κB activator PRMT5, and these complexes are confirmed by AlphaPulldown prediction. A complementary immunoprecipitation experiment recapitulates RELA interactions with other NF-κB factors, associating NF-κB inhibition with lower binding of NF-κB activators to RELA. This study describes a network of protumorigenic protein interactions and their rearrangement upon NF-κB inhibition with potential therapeutic implications in tumors with high NF- κB activity. This work was supported by the project National Institute for Cancer Research (Programme EXCELES, ID Project No. LX22NPO5102) - Funded by the European Union - Next Generation EU. (1) Lapcik, Petr, R- Greg Stacey, David Potesil, Petr Kulhanek, Leonard J. Foster, Pavel Bouchal, Global Interactome Mapping Reveals Pro-tumorigenic Interactions of NF-κB in Breast Cancer. Molecular & Cellular Proteomics 2024, 23, 4, 100744.

Návaznosti

LX22NPO5102, projekt VaV

Název: Národní ústav pro výzkum rakoviny (Akronym: NÚVR)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní ústav pro výzkum rakoviny, 5.1 EXCELES

Citovat

LAPČÍK, Petr; R. Greg STACEY; David POTĚŠIL; Petr KULHÁNEK; Leonard J. FOSTER a Pavel BOUCHAL. Global Interactome Mapping Reveals Protumorigenic Interactions of NF-κB in Breast Cancer. In In Book of Abstracts of Czech Annual Cancer Research Meeting, Olomouc. 24.-26.11.2025, p. 41-42. 2025. 2025.

@proceedings{2544008,
   author = {Lapčík, Petr and Stacey, R. Greg and Potěšil, David and Kulhánek, Petr and Foster, Leonard J. and Bouchal, Pavel},
   booktitle = {In Book of Abstracts of Czech Annual Cancer Research Meeting, Olomouc. 24.-26.11.2025, p. 41-42. 2025},
   language = {eng},
   title = {Global Interactome Mapping Reveals Protumorigenic Interactions of NF-κB in Breast Cancer},
   year = {2025}
}

TY  - CONF
ID  - 2544008
AU  - Lapčík, Petr - Stacey, R. Greg - Potěšil, David - Kulhánek, Petr - Foster, Leonard J. - Bouchal, Pavel
PY  - 2025
TI  - Global Interactome Mapping Reveals Protumorigenic Interactions of NF-κB in Breast Cancer
N2  - NF-κB pathway is involved in inflammation; however, recent data shows its role also in cancer development and progression, including metastasis. To understand the role of NF-κB interactome dynamics in cancer, in this work (1) we study the complexity of breast cancer interactome in luminal A breast cancer model and its rearrangement associated with NF-κB modulation. Liquid chromatography-mass spectrometry measurement of 160 size-exclusion chromatography fractions identifies 5460 protein groups. Seven thousand five hundred sixty eight interactions among these proteins have been reconstructed by PrInCE algorithm, of which 2564 have been validated in independent datasets. NF-κB modulation leads to rearrangement of protein complexes involved in NF-κB signaling and immune response, cell cycle regulation, and DNA replication. Central NF-κB transcription regulator RELA co-elutes with interactors of NF-κB activator PRMT5, and these complexes are confirmed by AlphaPulldown prediction. A complementary immunoprecipitation experiment recapitulates RELA interactions with other NF-κB factors, associating NF-κB inhibition with lower binding of NF-κB activators to RELA. This study describes a network of protumorigenic protein interactions and their rearrangement upon NF-κB inhibition with potential therapeutic implications in tumors with high NF- κB activity. This work was supported by the project National Institute for Cancer Research (Programme EXCELES, ID Project No. LX22NPO5102) - Funded by the European Union - Next Generation EU. (1) Lapcik, Petr, R- Greg Stacey, David Potesil, Petr Kulhanek, Leonard J. Foster, Pavel Bouchal, Global Interactome Mapping Reveals Pro-tumorigenic Interactions of NF-κB in Breast Cancer. Molecular & Cellular Proteomics 2024, 23, 4, 100744.
ER  -

LAPČÍK, Petr; R. Greg STACEY; David POTĚŠIL; Petr KULHÁNEK; Leonard J. FOSTER a Pavel BOUCHAL. Global Interactome Mapping Reveals Protumorigenic Interactions of NF-κB in Breast Cancer. In \textit{In Book of Abstracts of Czech Annual Cancer Research Meeting, Olomouc. 24.-26.11.2025, p. 41-42. 2025}. 2025.

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