Field potential duration and its variability as essential
parameters for revealing proarrhythmia: problematic aspects of
analysis in cardiomyocytes derived from human pluripotent stem
cells

J 2026

Field potential duration and its variability as essential parameters for revealing proarrhythmia: problematic aspects of analysis in cardiomyocytes derived from human pluripotent stem cells

KRÁL, Martin; Olga ŠVECOVÁ; Pavel JURAK; Josef HALAMEK; Milena ŠIMURDOVÁ et al.

Základní údaje

Originální název

Field potential duration and its variability as essential parameters for revealing proarrhythmia: problematic aspects of analysis in cardiomyocytes derived from human pluripotent stem cells

Autoři

KRÁL, Martin ; Olga ŠVECOVÁ ; Pavel JURAK; Josef HALAMEK; Milena ŠIMURDOVÁ; Jiří ŠIMURDA a Markéta BÉBAROVÁ

Vydání

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, OXFORD, PERGAMON-ELSEVIER SCIENCE LTD, 2026, 0079-6107

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30201 Cardiac and Cardiovascular systems

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.500 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Multielectrode array; Cardiomyocyte; Pluripotent stem cell; Field potential duration; Cycle length; Variability

Štítky

14110211, 14110515, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 29. 1. 2026 13:12, Mgr. Tereza Miškechová

Anotace

V originále

The microelectrode array (MEA) is an easy, high-throughput method, ideal for obtaining a large amount of data from excitable cells, including cardiomyocytes. However, the analysis can be problematic, especially the analysis of the field potential duration (FPD). Several factors, including the differentiation protocol, culture duration, recording settings, and signal processing, may influence the results. In this paper, we focused on the MEA recording settings, analysis, and evaluation of FPD from cardiomyocytes, especially those derived from human pluripotent stem cells (hPSC). By examining more than 120 original articles using MEA and any cardiac preparation, we detected an inconsistency in the acquisition setting. It is striking that only one-third of the studies provided complete information about filtering of the signal, even though this may substantially influence the shape of the signal and, thus, FPD. The performed analysis emphasizes a thorough inspection of both the 'raw' and filtered signals to estimate proper FPD values, as well as a careful determination of the relationship between FPD and cycle length before using any correction formula.

Návaznosti

MUNI/A/1641/2024, interní kód MU

Název: Od buňky k pacientovi II

Investor: Masarykova univerzita, Od buňky k pacientovi II

NU22-02-00348, projekt VaV

Název: Funkční hodnocení genetických variant u případů klinicky „skutečné“ idiopatické fibrilace komor: in vitro a in silico modelování s cílem odhalit arytmogenní mechanismus

Investor: Ministerstvo zdravotnictví ČR, Funkční hodnocení genetických variant u případů klinicky „skutečné“ idiopatické fibrilace komor: in vitro a in silico modelování s cílem odhalit arytmogenní mechanismus, Podprogram 1 - standardní

Citovat

KRÁL, Martin; Olga ŠVECOVÁ; Pavel JURAK; Josef HALAMEK; Milena ŠIMURDOVÁ; Jiří ŠIMURDA a Markéta BÉBAROVÁ. Field potential duration and its variability as essential parameters for revealing proarrhythmia: problematic aspects of analysis in cardiomyocytes derived from human pluripotent stem cells. PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2026, roč. 199, March 2026, s. 99-113. ISSN 0079-6107. Dostupné z: https://doi.org/10.1016/j.pbiomolbio.2025.12.004.

@article{2544920,
   author = {Král, Martin and Švecová, Olga and Jurak, Pavel and Halamek, Josef and Šimurdová, Milena and Šimurda, Jiří and Bébarová, Markéta},
   article_location = {OXFORD},
   article_number = {March 2026},
   doi = {https://doi.org/10.1016/j.pbiomolbio.2025.12.004},
   keywords = {Multielectrode array; Cardiomyocyte; Pluripotent stem cell; Field potential duration; Cycle length; Variability},
   language = {eng},
   issn = {0079-6107},
   journal = {PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY},
   title = {Field potential duration and its variability as essential parameters for revealing proarrhythmia: problematic aspects of analysis in cardiomyocytes derived from human pluripotent stem cells},
   url = {https://www.sciencedirect.com/science/article/pii/S0079610725000720?pes=vor&utm_source=clarivate&getft_integrator=clarivate},
   volume = {199},
   year = {2026}
}

TY  - JOUR
ID  - 2544920
AU  - Král, Martin - Švecová, Olga - Jurak, Pavel - Halamek, Josef - Šimurdová, Milena - Šimurda, Jiří - Bébarová, Markéta
PY  - 2026
TI  - Field potential duration and its variability as essential parameters for revealing proarrhythmia: problematic aspects of analysis in cardiomyocytes derived from human pluripotent stem cells
JF  - PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
VL  - 199
IS  - March 2026
SP  - 99-113
EP  - 99-113
PB  - PERGAMON-ELSEVIER SCIENCE LTD
SN  - 00796107
KW  - Multielectrode array
KW  - Cardiomyocyte
KW  - Pluripotent stem cell
KW  - Field potential duration
KW  - Cycle length
KW  - Variability
UR  - https://www.sciencedirect.com/science/article/pii/S0079610725000720?pes=vor&utm_source=clarivate&getft_integrator=clarivate
N2  - The microelectrode array (MEA) is an easy, high-throughput method, ideal for obtaining a large amount of data from excitable cells, including cardiomyocytes. However, the analysis can be problematic, especially the analysis of the field potential duration (FPD). Several factors, including the differentiation protocol, culture duration, recording settings, and signal processing, may influence the results. In this paper, we focused on the MEA recording settings, analysis, and evaluation of FPD from cardiomyocytes, especially those derived from human pluripotent stem cells (hPSC). By examining more than 120 original articles using MEA and any cardiac preparation, we detected an inconsistency in the acquisition setting. It is striking that only one-third of the studies provided complete information about filtering of the signal, even though this may substantially influence the shape of the signal and, thus, FPD. The performed analysis emphasizes a thorough inspection of both the 'raw' and filtered signals to estimate proper FPD values, as well as a careful determination of the relationship between FPD and cycle length before using any correction formula.
ER  -

KRÁL, Martin; Olga ŠVECOVÁ; Pavel JURAK; Josef HALAMEK; Milena ŠIMURDOVÁ; Jiří ŠIMURDA a Markéta BÉBAROVÁ. Field potential duration and its variability as essential parameters for revealing proarrhythmia: problematic aspects of analysis in cardiomyocytes derived from human pluripotent stem cells. \textit{PROGRESS IN BIOPHYSICS \&{}amp; MOLECULAR BIOLOGY}. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2026, roč.~199, March 2026, s.~99-113. ISSN~0079-6107. Dostupné z: https://doi.org/10.1016/j.pbiomolbio.2025.12.004.

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