Uukuniemi virus infection causes a pervasive remodelling of the
RNA-binding proteome in tick cells

J 2025

Uukuniemi virus infection causes a pervasive remodelling of the RNA-binding proteome in tick cells

WILSON, Alexandra Louise; Wael KAMEL; Kelsey DAVIES; Zaydah R. DE LAURENT; Rozeena ARIF et al.

Základní údaje

Originální název

Uukuniemi virus infection causes a pervasive remodelling of the RNA-binding proteome in tick cells

Autoři

Vydání

PLOS PATHOGENS, PUBLIC LIBRARY SCIENCE, 2025, 1553-7366

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10607 Virology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL, URL, URL

Impakt faktor

Impact factor: 4.900 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/25:00143279

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Transfection; RNA-binding proteins; Immunoprecipitation; Ticks; Viral replication; Virions; Protein folding; Cell cultures

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 1. 2026 10:10, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Cellular RNA-binding proteins (RBPs) are pivotal for the viral lifecycle, mediating key host-virus interactions that promote or repress virus infection. While these interactions have been largely studied in the vertebrate host, no comprehensive analyses of protein-RNA interactions occurring in cells of arbovirus vectors, in particular ticks, have been performed to date. Here we systematically identified the responses of the RNA-binding proteome (RBPome) to infection with a prototype bunyavirus (Uukuniemi virus; UUKV) in tick cells and discovered changes in RNA-binding activity for 283 proteins. In an orthogonal approach, we analysed the composition of the viral ribonucleoprotein by immunoprecipitation of UUKV nucleocapsid protein (N) in infected cells. We found many tick RBPs that are regulated by UUKV infection and associate with viral nucleocapsid protein complexes, and we confirmed experimentally that they impact UUKV infection. This includes the tick homolog of topoisomerase 3B (TOP3B), a protein able to manipulate the topology of RNA, which particularly affected viral particle production. Our data thus reveals the first protein-RNA interaction map for infected tick cells.

Citovat

WILSON, Alexandra Louise; Wael KAMEL; Kelsey DAVIES; Zaydah R. DE LAURENT; Rozeena ARIF; Andrew T. CLARKE; Lesley BELL-SAKYI; Douglas LAMONT; Yana DEMYANENKO; Marko NOERENBERG; Alain KOHL; Shabaz MOHAMMED; Alfredo CASTELLO a Benjamin BRENNAN. Uukuniemi virus infection causes a pervasive remodelling of the RNA-binding proteome in tick cells. PLOS PATHOGENS. PUBLIC LIBRARY SCIENCE, 2025, roč. 21, č. 8, s. 1-26. ISSN 1553-7366. Dostupné z: https://doi.org/10.1371/journal.ppat.1013393.

@article{2546969,
   author = {Wilson, Alexandra Louise and Kamel, Wael and Davies, Kelsey and De Laurent, Zaydah R. and Arif, Rozeena and Clarke, Andrew T. and BellandSakyi, Lesley and Lamont, Douglas and Demyanenko, Yana and Noerenberg, Marko and Kohl, Alain and Mohammed, Shabaz and Castello, Alfredo and Brennan, Benjamin},
   article_number = {8},
   doi = {https://doi.org/10.1371/journal.ppat.1013393},
   keywords = {Transfection; RNA-binding proteins; Immunoprecipitation; Ticks; Viral replication; Virions; Protein folding; Cell cultures},
   language = {eng},
   issn = {1553-7366},
   journal = {PLOS PATHOGENS},
   title = {Uukuniemi virus infection causes a pervasive remodelling of the RNA-binding proteome in tick cells},
   url = {http://dx.doi.org/10.5525/gla.researchdata.1983},
   volume = {21},
   year = {2025}
}

TY  - JOUR
ID  - 2546969
AU  - Wilson, Alexandra Louise - Kamel, Wael - Davies, Kelsey - De Laurent, Zaydah R. - Arif, Rozeena - Clarke, Andrew T. - Bell-Sakyi, Lesley - Lamont, Douglas - Demyanenko, Yana - Noerenberg, Marko - Kohl, Alain - Mohammed, Shabaz - Castello, Alfredo - Brennan, Benjamin
PY  - 2025
TI  - Uukuniemi virus infection causes a pervasive remodelling of the RNA-binding proteome in tick cells
JF  - PLOS PATHOGENS
VL  - 21
IS  - 8
SP  - 1-26
EP  - 1-26
PB  - PUBLIC LIBRARY SCIENCE
SN  - 15537366
KW  - Transfection
KW  - RNA-binding proteins
KW  - Immunoprecipitation
KW  - Ticks
KW  - Viral replication
KW  - Virions
KW  - Protein folding
KW  - Cell cultures
UR  - http://dx.doi.org/10.5525/gla.researchdata.1983
N2  - Cellular RNA-binding proteins (RBPs) are pivotal for the viral lifecycle, mediating key host-virus interactions that promote or repress virus infection. While these interactions have been largely studied in the vertebrate host, no comprehensive analyses of protein-RNA interactions occurring in cells of arbovirus vectors, in particular ticks, have been performed to date. Here we systematically identified the responses of the RNA-binding proteome (RBPome) to infection with a prototype bunyavirus (Uukuniemi virus; UUKV) in tick cells and discovered changes in RNA-binding activity for 283 proteins. In an orthogonal approach, we analysed the composition of the viral ribonucleoprotein by immunoprecipitation of UUKV nucleocapsid protein (N) in infected cells. We found many tick RBPs that are regulated by UUKV infection and associate with viral nucleocapsid protein complexes, and we confirmed experimentally that they impact UUKV infection. This includes the tick homolog of topoisomerase 3B (TOP3B), a protein able to manipulate the topology of RNA, which particularly affected viral particle production. Our data thus reveals the first protein-RNA interaction map for infected tick cells.
ER  -

WILSON, Alexandra Louise; Wael KAMEL; Kelsey DAVIES; Zaydah R. DE LAURENT; Rozeena ARIF; Andrew T. CLARKE; Lesley BELL-SAKYI; Douglas LAMONT; Yana DEMYANENKO; Marko NOERENBERG; Alain KOHL; Shabaz MOHAMMED; Alfredo CASTELLO a Benjamin BRENNAN. Uukuniemi virus infection causes a pervasive remodelling of the RNA-binding proteome in tick cells. \textit{PLOS PATHOGENS}. PUBLIC LIBRARY SCIENCE, 2025, roč.~21, č.~8, s.~1-26. ISSN~1553-7366. Dostupné z: https://doi.org/10.1371/journal.ppat.1013393.

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