Wildlife Sentinel: Development of Multispecies Protein A-ELISA
for Detection of SARS-CoV-2 Antibodies in Zoo Animals as a
Proof of Concept for Wildlife Surveillance

J 2025

Wildlife Sentinel: Development of Multispecies Protein A-ELISA for Detection of SARS-CoV-2 Antibodies in Zoo Animals as a Proof of Concept for Wildlife Surveillance

ITALIYA, Jignesh; Petra STRAKOVÁ; Lukas PAVLACIK; Jiri VAHALA; Jaroslav Haimy HYJANEK et al.

Základní údaje

Originální název

Wildlife Sentinel: Development of Multispecies Protein A-ELISA for Detection of SARS-CoV-2 Antibodies in Zoo Animals as a Proof of Concept for Wildlife Surveillance

Autoři

ITALIYA, Jignesh; Petra STRAKOVÁ; Lukas PAVLACIK; Jiri VAHALA; Jaroslav Haimy HYJANEK; Jiří SALÁT; Daniel RŮŽEK; Dominika KOMARKOVA a Jiri CERNY

Vydání

JOURNAL OF WILDLIFE DISEASES, LAWRENCE, WILDLIFE DISEASE ASSOC, INC, 2025, 0090-3558

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.200 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/25:00143297

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

leopard pardus COVID-19; indirect ELISA; proof of concept; SARS-CoV-2; serologic surveillance; virus neutralization test; wildlife surveillance; zoo animals

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 1. 2026 08:34, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), first emerged in Wuhan in 2019 and rapidly spread worldwide. During the course of the COVID-19 pandemic, numerous reports highlighted infections of wild animals by SARS-CoV-2. Nevertheless, further research is required to understand the virus potential to infect various animal species, which is crucial for evaluating its future evolution and the potential reemergence of SARS-CoV-2. The total concentration of immunoglobulin G (IgG) represents a valuable yet underused diagnostic parameter for health assessments in wild animals, primarily due to the absence of effective diagnostic tools. A protein A-based indirect ELISA can serve as an efficient method to identify IgG antibodies against different pathogens in wildlife surveillance programs. To develop a multispecies protein A-ELISA for IgG detection against SARS-CoV-2, we used 44 animal species serum samples to ascertain the protein A binding affinity, and 88 serum samples, chosen for the strong binding affinity to protein A, were used to identify IgG antibodies against SARS-CoV-2. The serum samples were obtained from animals housed in Safari Park Dvu & ring;r Kralove, Czech Republic. The zoo animals are in proximity to humans, facilitating the exploration of potential reverse transmission events of SARS-CoV-2 from humans to animals. Also, they undergo routine veterinary examinations, providing convenient access to blood samples. Therefore, they can be easily used for the development of protein A-based indirect ELISA for wildlife disease surveillance programs. Based on the ELISA results, antibodies to SARS-CoV-2 were detected in the sera of 16 animals. To further confirm these findings, the ELISApositive samples were subjected to virus neutralization assays. This additional testing revealed the presence of SARS-CoV-2 neutralizing antibodies in the serum of two white rhinoceros (Ceratotherium simum simum) and one Persian leopard (Panthera pardus tulliana).

Citovat

ITALIYA, Jignesh; Petra STRAKOVÁ; Lukas PAVLACIK; Jiri VAHALA; Jaroslav Haimy HYJANEK; Jiří SALÁT; Daniel RŮŽEK; Dominika KOMARKOVA a Jiri CERNY. Wildlife Sentinel: Development of Multispecies Protein A-ELISA for Detection of SARS-CoV-2 Antibodies in Zoo Animals as a Proof of Concept for Wildlife Surveillance. JOURNAL OF WILDLIFE DISEASES. LAWRENCE: WILDLIFE DISEASE ASSOC, INC, 2025, roč. 61, č. 2, s. 396-407. ISSN 0090-3558. Dostupné z: https://doi.org/10.7589/JWD-D-24-00028.

@article{2547238,
   author = {Italiya, Jignesh and Straková, Petra and Pavlacik, Lukas and Vahala, Jiri and Hyjanek, Jaroslav Haimy and Salát, Jiří and Růžek, Daniel and Komarkova, Dominika and Cerny, Jiri},
   article_location = {LAWRENCE},
   article_number = {2},
   doi = {https://doi.org/10.7589/JWD-D-24-00028},
   keywords = {leopard pardus COVID-19; indirect ELISA; proof of concept; SARS-CoV-2; serologic surveillance; virus neutralization test; wildlife surveillance; zoo animals},
   language = {eng},
   issn = {0090-3558},
   journal = {JOURNAL OF WILDLIFE DISEASES},
   title = {Wildlife Sentinel: Development of Multispecies Protein A-ELISA for Detection of SARS-CoV-2 Antibodies in Zoo Animals as a Proof of Concept for Wildlife Surveillance},
   url = {https://bioone.org/journals/journal-of-wildlife-diseases/volume-61/issue-2/JWD-D-24-00028/WILDLIFE-SENTINEL--DEVELOPMENT-OF-MULTISPECIES-PROTEIN-A-ELISA-FOR/10.7589/JWD-D-24-00028.short},
   volume = {61},
   year = {2025}
}

TY  - JOUR
ID  - 2547238
AU  - Italiya, Jignesh - Straková, Petra - Pavlacik, Lukas - Vahala, Jiri - Hyjanek, Jaroslav Haimy - Salát, Jiří - Růžek, Daniel - Komarkova, Dominika - Cerny, Jiri
PY  - 2025
TI  - Wildlife Sentinel: Development of Multispecies Protein A-ELISA for Detection of SARS-CoV-2 Antibodies in Zoo Animals as a Proof of Concept for Wildlife Surveillance
JF  - JOURNAL OF WILDLIFE DISEASES
VL  - 61
IS  - 2
SP  - 396-407
EP  - 396-407
PB  - WILDLIFE DISEASE ASSOC, INC
SN  - 00903558
KW  - leopard pardus COVID-19
KW  - indirect ELISA
KW  - proof of concept
KW  - SARS-CoV-2
KW  - serologic surveillance
KW  - virus neutralization test
KW  - wildlife surveillance
KW  - zoo animals
UR  - https://bioone.org/journals/journal-of-wildlife-diseases/volume-61/issue-2/JWD-D-24-00028/WILDLIFE-SENTINEL--DEVELOPMENT-OF-MULTISPECIES-PROTEIN-A-ELISA-FOR/10.7589/JWD-D-24-00028.short
N2  - Coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), first emerged in Wuhan in 2019 and rapidly spread worldwide. During the course of the COVID-19 pandemic, numerous reports highlighted infections of wild animals by SARS-CoV-2. Nevertheless, further research is required to understand the virus potential to infect various animal species, which is crucial for evaluating its future evolution and the potential reemergence of SARS-CoV-2. The total concentration of immunoglobulin G (IgG) represents a valuable yet underused diagnostic parameter for health assessments in wild animals, primarily due to the absence of effective diagnostic tools. A protein A-based indirect ELISA can serve as an efficient method to identify IgG antibodies against different pathogens in wildlife surveillance programs. To develop a multispecies protein A-ELISA for IgG detection against SARS-CoV-2, we used 44 animal species serum samples to ascertain the protein A binding affinity, and 88 serum samples, chosen for the strong binding affinity to protein A, were used to identify IgG antibodies against SARS-CoV-2. The serum samples were obtained from animals housed in Safari Park Dvu & ring;r Kralove, Czech Republic. The zoo animals are in proximity to humans, facilitating the exploration of potential reverse transmission events of SARS-CoV-2 from humans to animals. Also, they undergo routine veterinary examinations, providing convenient access to blood samples. Therefore, they can be easily used for the development of protein A-based indirect ELISA for wildlife disease surveillance programs. Based on the ELISA results, antibodies to SARS-CoV-2 were detected in the sera of 16 animals. To further confirm these findings, the ELISApositive samples were subjected to virus neutralization assays. This additional testing revealed the presence of SARS-CoV-2 neutralizing antibodies in the serum of two white rhinoceros (Ceratotherium simum simum) and one Persian leopard (Panthera pardus tulliana).
ER  -

ITALIYA, Jignesh; Petra STRAKOVÁ; Lukas PAVLACIK; Jiri VAHALA; Jaroslav Haimy HYJANEK; Jiří SALÁT; Daniel RŮŽEK; Dominika KOMARKOVA a Jiri CERNY. Wildlife Sentinel: Development of Multispecies Protein A-ELISA for Detection of SARS-CoV-2 Antibodies in Zoo Animals as a Proof of Concept for Wildlife Surveillance. \textit{JOURNAL OF WILDLIFE DISEASES}. LAWRENCE: WILDLIFE DISEASE ASSOC, INC, 2025, roč.~61, č.~2, s.~396-407. ISSN~0090-3558. Dostupné z: https://doi.org/10.7589/JWD-D-24-00028.

Přehled o publikaci