Multicolor Upconversion Forster Resonant Energy Transfer Using
Optimized Yb@YbTm Core@Shell Nanoparticles

J 2025

Multicolor Upconversion Forster Resonant Energy Transfer Using Optimized Yb@YbTm Core@Shell Nanoparticles

BEKARSKI, Grzegorz; Katarzyna PROROK; František ŠTĚTINA; Malgorzata MISIAK; Hans-Heiner GORRIS et al.

Základní údaje

Originální název

Multicolor Upconversion Forster Resonant Energy Transfer Using Optimized Yb@YbTm Core@Shell Nanoparticles

Autoři

BEKARSKI, Grzegorz; Katarzyna PROROK; František ŠTĚTINA; Malgorzata MISIAK; Hans-Heiner GORRIS a Artur BEDNARKIEWICZ

Vydání

ACS NANO, WASHINGTON, AMER CHEMICAL SOC, 2025, 1936-0851

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30403 Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 16.000 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

upconversion; FRET; nanomaterials; multiplexing; sensing

Štítky

14313050, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 1. 2026 14:23, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Upconverting nanoparticles (UCNPs) have emerged as promising alternative donors for resonance energy transfer (FRET)-based biosensing. However, employing UCNPs in FRET assays remains challenging because they display relatively small absorption cross sections and are relatively large as compared to the Forster distance. Thousands of individual donor ions in each UCNP are located within various distances from surface-bound acceptors, complicating the data analysis. While previous studies have explored how the composition and architecture of UCNPs influence FRET, many reports remain qualitative, and multicolor UC-FRET systems involving a single donor and multiple acceptors are less commonly studied than single-donor-single-acceptor systems. To address these challenges, we synthesized UCNPs with an absorbing core (Yb3+-doped)/active shell (Yb3+, Tm3+-doped) nanoparticles systematically varying Tm3+ concentrations to optimize the FRET efficiency to surface-bound organic acceptors. A shell composition containing 4% Tm3+ yielded the highest FRET efficiency. Moreover, four distinct ATTO dyes showing spectral overlap with the blue emission of Tm3+ were used as acceptor dyes on the surface of UCNPs to evaluate FRET efficiencies in spectral and time domains. The differentiation of the four ATTO dyes on one type of upconverting donor nanoparticles using a simple ratiometric approach lays the foundation for the design of multiplexed bioassays. Our results offer a strategy for improving UC-FRET sensitivity through smart core-shell UCNPs designs, donor concentration tuning, and provide important insights into the rational design of more efficient, multicolor, and wash-free UC biosensing platforms.

Návaznosti

GF23-06199K, projekt VaV

Název: Nové anti-Stokesové lanthanoidové nanočástice a vícebarevný FRET mechanismy pro jednomolekulové sekvenování DNA

Investor: Grantová agentura ČR, Nové anti-Stokesové upkonverzní nanočástice a vícebarevný FRET pro jednomolekulové sekvenování DNA, Lead agentura

Citovat

BEKARSKI, Grzegorz; Katarzyna PROROK; František ŠTĚTINA; Malgorzata MISIAK; Hans-Heiner GORRIS a Artur BEDNARKIEWICZ. Multicolor Upconversion Forster Resonant Energy Transfer Using Optimized Yb@YbTm Core@Shell Nanoparticles. ACS NANO. WASHINGTON: AMER CHEMICAL SOC, 2025, roč. 19, č. 48, s. 41110-41120. ISSN 1936-0851. Dostupné z: https://doi.org/10.1021/acsnano.5c13869.

@article{2548631,
   author = {Bekarski, Grzegorz and Prorok, Katarzyna and Štětina, František and Misiak, Malgorzata and Gorris, HansandHeiner and Bednarkiewicz, Artur},
   article_location = {WASHINGTON},
   article_number = {48},
   doi = {https://doi.org/10.1021/acsnano.5c13869},
   keywords = {upconversion; FRET; nanomaterials; multiplexing; sensing},
   language = {eng},
   issn = {1936-0851},
   journal = {ACS NANO},
   title = {Multicolor Upconversion Forster Resonant Energy Transfer Using Optimized Yb@YbTm Core@Shell Nanoparticles},
   url = {https://pubs.acs.org/doi/10.1021/acsnano.5c13869},
   volume = {19},
   year = {2025}
}

TY  - JOUR
ID  - 2548631
AU  - Bekarski, Grzegorz - Prorok, Katarzyna - Štětina, František - Misiak, Malgorzata - Gorris, Hans-Heiner - Bednarkiewicz, Artur
PY  - 2025
TI  - Multicolor Upconversion Forster Resonant Energy Transfer Using Optimized Yb@YbTm Core@Shell Nanoparticles
JF  - ACS NANO
VL  - 19
IS  - 48
SP  - 41110-41120
EP  - 41110-41120
PB  - AMER CHEMICAL SOC
SN  - 19360851
KW  - upconversion
KW  - FRET
KW  - nanomaterials
KW  - multiplexing
KW  - sensing
UR  - https://pubs.acs.org/doi/10.1021/acsnano.5c13869
N2  - Upconverting nanoparticles (UCNPs) have emerged as promising alternative donors for resonance energy transfer (FRET)-based biosensing. However, employing UCNPs in FRET assays remains challenging because they display relatively small absorption cross sections and are relatively large as compared to the Forster distance. Thousands of individual donor ions in each UCNP are located within various distances from surface-bound acceptors, complicating the data analysis. While previous studies have explored how the composition and architecture of UCNPs influence FRET, many reports remain qualitative, and multicolor UC-FRET systems involving a single donor and multiple acceptors are less commonly studied than single-donor-single-acceptor systems. To address these challenges, we synthesized UCNPs with an absorbing core (Yb3+-doped)/active shell (Yb3+, Tm3+-doped) nanoparticles systematically varying Tm3+ concentrations to optimize the FRET efficiency to surface-bound organic acceptors. A shell composition containing 4% Tm3+ yielded the highest FRET efficiency. Moreover, four distinct ATTO dyes showing spectral overlap with the blue emission of Tm3+ were used as acceptor dyes on the surface of UCNPs to evaluate FRET efficiencies in spectral and time domains. The differentiation of the four ATTO dyes on one type of upconverting donor nanoparticles using a simple ratiometric approach lays the foundation for the design of multiplexed bioassays. Our results offer a strategy for improving UC-FRET sensitivity through smart core-shell UCNPs designs, donor concentration tuning, and provide important insights into the rational design of more efficient, multicolor, and wash-free UC biosensing platforms.
ER  -

BEKARSKI, Grzegorz; Katarzyna PROROK; František ŠTĚTINA; Malgorzata MISIAK; Hans-Heiner GORRIS a Artur BEDNARKIEWICZ. Multicolor Upconversion Forster Resonant Energy Transfer Using Optimized Yb@YbTm Core@Shell Nanoparticles. \textit{ACS NANO}. WASHINGTON: AMER CHEMICAL SOC, 2025, roč.~19, č.~48, s.~41110-41120. ISSN~1936-0851. Dostupné z: https://doi.org/10.1021/acsnano.5c13869.

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