The role of cellulose nanoparticles in enhancing human iPSC
compatibility with composite conductive PANI/cellulose films

J 2025

The role of cellulose nanoparticles in enhancing human iPSC compatibility with composite conductive PANI/cellulose films

KORABKOVA, Eva; Vera KASPARKOVA; Jiří PACHERNÍK; Miroslava TRCHOVA; Kristyna VALASKOVA et al.

Základní údaje

Originální název

The role of cellulose nanoparticles in enhancing human iPSC compatibility with composite conductive PANI/cellulose films

Autoři

Vydání

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, AMSTERDAM, ELSEVIER, 2025, 0141-8130

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10404 Polymer science

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 8.500 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/25:00144641

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Polyaniline films; Cellulose; Composite; Antibacterial activity; Human iPSC cells

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 1. 2026 11:18, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The development of composites with tailored surface properties and electrical conductivity is critical for various biomedical applications. However, a substantial gap remains in understanding how the unique properties of cellulose, such as biocompatibility and renewability can be effectively combined with those of polyaniline (PANI), including electrical conductivity, antibacterial, and antioxidant activity, to create composite films with advanced multifunctional performance. Indeed, conductivity can be used not only to monitor biological functions but also as a cell instructive factor. To meet these requirements, thin composite films were synthetized using oxidative polymerization of aniline hydrochloride with ammonium peroxydisulfate in the presence of either cellulose nanocrystals (CNC) or cellulose nanofibres (CNF). Their cytocompatibility was demonstrated with the NIH/3 T3 fibroblast line and highly progressive human induced pluripotent stem cells. The films also showed antibacterial activity against Staphyloccocus aureus and Escherichia coli, surpassing that of pristine PANI and meeting the EN ISO 20743 criteria for materials with significant activity (reducing CFU value to zero). Comprehensive physicochemical characterization revealed that the films possessed exceptional DPPH radical scavenging achieving their complete (100 %) removal within 15 min, and electrical conductivity within units of S cm−1. Raman spectroscopy showed that PANI/CNC composites were more resistant to deprotonation caused by laser illumination than PANI/CNF, which resulted from the presence of sulfate groups on the CNC surface. These findings highlight that PANI/CNC and PANI/CNF films are promising materials for applications requiring surfaces that are simultaneously biocompatible, electrically conductive, and antibacterial.

Návaznosti

GA23-07425S, projekt VaV

Název: Anisotropní a elektricky vodivé biomateriály

Investor: Grantová agentura ČR, Elektricky vodivé biomateriály

Citovat

KORABKOVA, Eva; Vera KASPARKOVA; Jiří PACHERNÍK; Miroslava TRCHOVA; Kristyna VALASKOVA; Robert MOUCKA; Štefan ZELENÁK; Kadir OZALTIN; Lucie URBANKOVA; Adam SRNEC; Zdenka VICHOVA; Jan VICHA; Marketa KADLECKOVA a Petr HUMPOLICEK. The role of cellulose nanoparticles in enhancing human iPSC compatibility with composite conductive PANI/cellulose films. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES. AMSTERDAM: ELSEVIER, 2025, roč. 330, November, s. 1-12. ISSN 0141-8130. Dostupné z: https://doi.org/10.1016/j.ijbiomac.2025.147959.

@article{2550866,
   author = {Korabkova, Eva and Kasparkova, Vera and Pacherník, Jiří and Trchova, Miroslava and Valaskova, Kristyna and Moucka, Robert and Zelenák, Štefan and Ozaltin, Kadir and Urbankova, Lucie and Srnec, Adam and Vichova, Zdenka and Vicha, Jan and Kadleckova, Marketa and Humpolicek, Petr},
   article_location = {AMSTERDAM},
   article_number = {November},
   doi = {https://doi.org/10.1016/j.ijbiomac.2025.147959},
   keywords = {Polyaniline films; Cellulose; Composite; Antibacterial activity; Human iPSC cells},
   language = {eng},
   issn = {0141-8130},
   journal = {INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES},
   title = {The role of cellulose nanoparticles in enhancing human iPSC compatibility with composite conductive PANI/cellulose films},
   url = {https://www.sciencedirect.com/science/article/pii/S0141813025085162},
   volume = {330},
   year = {2025}
}

TY  - JOUR
ID  - 2550866
AU  - Korabkova, Eva - Kasparkova, Vera - Pacherník, Jiří - Trchova, Miroslava - Valaskova, Kristyna - Moucka, Robert - Zelenák, Štefan - Ozaltin, Kadir - Urbankova, Lucie - Srnec, Adam - Vichova, Zdenka - Vicha, Jan - Kadleckova, Marketa - Humpolicek, Petr
PY  - 2025
TI  - The role of cellulose nanoparticles in enhancing human iPSC compatibility with composite conductive PANI/cellulose films
JF  - INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
VL  - 330
IS  - November
SP  - 1-12
EP  - 1-12
PB  - ELSEVIER
SN  - 01418130
KW  - Polyaniline films
KW  - Cellulose
KW  - Composite
KW  - Antibacterial activity
KW  - Human iPSC cells
UR  - https://www.sciencedirect.com/science/article/pii/S0141813025085162
N2  - The development of composites with tailored surface properties and electrical conductivity is critical for various biomedical applications. However, a substantial gap remains in understanding how the unique properties of cellulose, such as biocompatibility and renewability can be effectively combined with those of polyaniline (PANI), including electrical conductivity, antibacterial, and antioxidant activity, to create composite films with advanced multifunctional performance. Indeed, conductivity can be used not only to monitor biological functions but also as a cell instructive factor. To meet these requirements, thin composite films were synthetized using oxidative polymerization of aniline hydrochloride with ammonium peroxydisulfate in the presence of either cellulose nanocrystals (CNC) or cellulose nanofibres (CNF). Their cytocompatibility was demonstrated with the NIH/3 T3 fibroblast line and highly progressive human induced pluripotent stem cells. The films also showed antibacterial activity against Staphyloccocus aureus and Escherichia coli, surpassing that of pristine PANI and meeting the EN ISO 20743 criteria for materials with significant activity (reducing CFU value to zero). Comprehensive physicochemical characterization revealed that the films possessed exceptional DPPH radical scavenging achieving their complete (100 %) removal within 15 min, and electrical conductivity within units of S cm−1. Raman spectroscopy showed that PANI/CNC composites were more resistant to deprotonation caused by laser illumination than PANI/CNF, which resulted from the presence of sulfate groups on the CNC surface. These findings highlight that PANI/CNC and PANI/CNF films are promising materials for applications requiring surfaces that are simultaneously biocompatible, electrically conductive, and antibacterial.
ER  -

KORABKOVA, Eva; Vera KASPARKOVA; Jiří PACHERNÍK; Miroslava TRCHOVA; Kristyna VALASKOVA; Robert MOUCKA; Štefan ZELENÁK; Kadir OZALTIN; Lucie URBANKOVA; Adam SRNEC; Zdenka VICHOVA; Jan VICHA; Marketa KADLECKOVA a Petr HUMPOLICEK. The role of cellulose nanoparticles in enhancing human iPSC compatibility with composite conductive PANI/cellulose films. \textit{INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES}. AMSTERDAM: ELSEVIER, 2025, roč.~330, November, s.~1-12. ISSN~0141-8130. Dostupné z: https://doi.org/10.1016/j.ijbiomac.2025.147959.

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