Occurrence, growth, and virulence genes of Bacillus cereus in
ready-to-cook plant-based meat analogues

J 2025

Occurrence, growth, and virulence genes of Bacillus cereus in ready-to-cook plant-based meat analogues

DUŠKOVÁ, Marta; Josef KAMENÍK; Michaela ČUTOVÁ; Kateřina DOROTÍKOVÁ; Michaela KRÁLOVÁ et al.

Základní údaje

Originální název

Occurrence, growth, and virulence genes of Bacillus cereus in ready-to-cook plant-based meat analogues

Autoři

DUŠKOVÁ, Marta; Josef KAMENÍK; Michaela ČUTOVÁ; Kateřina DOROTÍKOVÁ; Michaela KRÁLOVÁ a Ondrej ŠEDO

Vydání

ACTA VETERINARIA BRNO, PALACKEHO 1-3, BRNO CS-612 42, CZECH REP, University of Veterinary and Pharmaceuti, 2025, 0001-7213

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40301 Veterinary science

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 0.700 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/25:00143603

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

total visible count; heat treatment; hbl genes; nhe genes; ces gene; Bacillus cytotoxicus

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 3. 2026 22:52, Mgr. Eva Dubská

Anotace

V originále

The aim of this study was to monitor the growth of Bacillus cereus in 11 different types of naturally contaminated ready-to-cook plant-based meat analogues (PBMAs). The PBMAs were prepared according to the manufacturer's instructions and subsequently stored at room temperature for up to 24 h. Bacillus spp. represented the second most numerous group of microbiota detected in PBMAs after lactic acid bacteria. Bacillus cereus sensu lato (s.l.) was isolated from all 11 PBMA samples. Following heat treatment and 24-h storage at 22 degrees C, B. cereus s.l. was detected by plate counting in five samples, with one product reaching nearly 4 log CFU/g. The presence of B. cereus sensu stricto (s.s.) was detected in all 11 samples. Bacillus thuringiensis was present in 7 samples, and in 4 PBMA samples the species B. weihenstephanensis, B. mycoides, andB. cytotoxicus were found. Atotal of 95.7% ofB. cereus s.l. strains contained genes for toxin production. The presence of nhe genes was demonstrated in 92.5%, hbl genes in 59.0% of the analysed B. cereus s.l. strains, the cytK-1 gene in 5 strains (B. cytotoxicus). The presence of the ces gene was confirmed in 5% of B. cereus s.l. strains. In total, 47 different combinations of genes for toxin production were detected in B. cereus s.l. strains. The almost 100% presence of virulence genes associated with the possibility ofB. cereus growth during inappropriate storage of PBMAs thus poses ariskforthe development of foodborne diseases.

Návaznosti

EH23_015/0008175, projekt VaV

Název: Inovace České infrastruktury pro integrativní strukturní biologii

90242, velká výzkumná infrastruktura

Název: CIISB III

Citovat

DUŠKOVÁ, Marta; Josef KAMENÍK; Michaela ČUTOVÁ; Kateřina DOROTÍKOVÁ; Michaela KRÁLOVÁ a Ondrej ŠEDO. Occurrence, growth, and virulence genes of Bacillus cereus in ready-to-cook plant-based meat analogues. ACTA VETERINARIA BRNO. PALACKEHO 1-3, BRNO CS-612 42, CZECH REP: University of Veterinary and Pharmaceuti, 2025, roč. 94, č. 4, s. 317-327. ISSN 0001-7213. Dostupné z: https://doi.org/10.2754/avb202594040317.

@article{2551088,
   author = {Dušková, Marta and Kameník, Josef and Čutová, Michaela and Dorotíková, Kateřina and Králová, Michaela and Šedo, Ondrej},
   article_location = {PALACKEHO 1-3, BRNO CS-612 42, CZECH REP},
   article_number = {4},
   doi = {https://doi.org/10.2754/avb202594040317},
   keywords = {total visible count; heat treatment; hbl genes; nhe genes; ces gene; Bacillus cytotoxicus},
   language = {eng},
   issn = {0001-7213},
   journal = {ACTA VETERINARIA BRNO},
   title = {Occurrence, growth, and virulence genes of Bacillus cereus in ready-to-cook plant-based meat analogues},
   url = {https://actavet.vfu.cz/94/4/0317/},
   volume = {94},
   year = {2025}
}

TY  - JOUR
ID  - 2551088
AU  - Dušková, Marta - Kameník, Josef - Čutová, Michaela - Dorotíková, Kateřina - Králová, Michaela - Šedo, Ondrej
PY  - 2025
TI  - Occurrence, growth, and virulence genes of Bacillus cereus in ready-to-cook plant-based meat analogues
JF  - ACTA VETERINARIA BRNO
VL  - 94
IS  - 4
SP  - 317-327
EP  - 317-327
PB  - University of Veterinary and Pharmaceuti
SN  - 00017213
KW  - total visible count
KW  - heat treatment
KW  - hbl genes
KW  - nhe genes
KW  - ces gene
KW  - Bacillus cytotoxicus
UR  - https://actavet.vfu.cz/94/4/0317/
N2  - The aim of this study was to monitor the growth of Bacillus cereus in 11 different types of naturally contaminated ready-to-cook plant-based meat analogues (PBMAs). The PBMAs were prepared according to the manufacturer's instructions and subsequently stored at room temperature for up to 24 h. Bacillus spp. represented the second most numerous group of microbiota detected in PBMAs after lactic acid bacteria. Bacillus cereus sensu lato (s.l.) was isolated from all 11 PBMA samples. Following heat treatment and 24-h storage at 22 degrees C, B. cereus s.l. was detected by plate counting in five samples, with one product reaching nearly 4 log CFU/g. The presence of B. cereus sensu stricto (s.s.) was detected in all 11 samples. Bacillus thuringiensis was present in 7 samples, and in 4 PBMA samples the species B. weihenstephanensis, B. mycoides, andB. cytotoxicus were found. Atotal of 95.7% ofB. cereus s.l. strains contained genes for toxin production. The presence of nhe genes was demonstrated in 92.5%, hbl genes in 59.0% of the analysed B. cereus s.l. strains, the cytK-1 gene in 5 strains (B. cytotoxicus). The presence of the ces gene was confirmed in 5% of B. cereus s.l. strains. In total, 47 different combinations of genes for toxin production were detected in B. cereus s.l. strains. The almost 100% presence of virulence genes associated with the possibility ofB. cereus growth during inappropriate storage of PBMAs thus poses ariskforthe development of foodborne diseases.
ER  -

DUŠKOVÁ, Marta; Josef KAMENÍK; Michaela ČUTOVÁ; Kateřina DOROTÍKOVÁ; Michaela KRÁLOVÁ a Ondrej ŠEDO. Occurrence, growth, and virulence genes of Bacillus cereus in ready-to-cook plant-based meat analogues. \textit{ACTA VETERINARIA BRNO}. PALACKEHO 1-3, BRNO CS-612 42, CZECH REP: University of Veterinary and Pharmaceuti, 2025, roč.~94, č.~4, s.~317-327. ISSN~0001-7213. Dostupné z: https://doi.org/10.2754/avb202594040317.

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