Methods for Evaluation of Antivirals and Neutralizing
Antibodies Against Tick-Borne Encephalitis Virus

C 2025

Methods for Evaluation of Antivirals and Neutralizing Antibodies Against Tick-Borne Encephalitis Virus

EYER, Luděk; Pavel SVOBODA; Martin PALUS; Jiří SALÁT; Daniel RŮŽEK et al.

Základní údaje

Originální název

Methods for Evaluation of Antivirals and Neutralizing Antibodies Against Tick-Borne Encephalitis Virus

Autoři

EYER, Luděk; Pavel SVOBODA; Martin PALUS; Jiří SALÁT a Daniel RŮŽEK

Vydání

1. vyd. New York, Tick-Borne Viruses : Methods and Protocols, od s. 125-136, 12 s. Methods in Molecular Biology, 2025

Nakladatel

Humana Press Inc.

Další údaje

Jazyk

angličtina

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Obor

10607 Virology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Odkazy

URL

Označené pro přenos do RIV

Ano

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-1-0716-4586-4

DOI

https://doi.org/10.1007/978-1-0716-4587-1_14

EID Scopus

2-s2.0-105008746045

Klíčová slova anglicky

Antivirals; Immunofluorescence; Monoclonal antibodies; Mouse infection; Neutralization test; Plaque assay; Tick-borne encephalitis virus

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 2. 2026 14:59, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Tick-borne encephalitis (TBE) is a potentially fatal neuroinfection of humans caused by the TBE virus. There is no specific therapy for TBE, as treatment is only supportive. Therefore, efforts to develop an effective specific therapy for TBE are warranted. Small molecule inhibitors and monoclonal antibodies are under intense investigation as potential therapeutics to combat TBE in humans. Here we describe the basic methods that can be used to test small molecule antivirals or monoclonal antibodies against TBEV. These include in vitro methods such as plaque assays, neutralizing assays, immunofluorescence staining of viral antigen, and cell-based antiviral assays, as well as in vivo assays in mouse model of TBE.

Návaznosti

NU22-05-00659, projekt VaV

Název: Neutralizační nanoprotilátky jako potenciální terapeutika klíšťové encefalitidy

Investor: Ministerstvo zdravotnictví ČR, Neutralizační nanoprotilátky jako potenciální terapeutika klíšťové encefalitidy, Podprogram 1 - standardní

Citovat

EYER, Luděk; Pavel SVOBODA; Martin PALUS; Jiří SALÁT a Daniel RŮŽEK. Methods for Evaluation of Antivirals and Neutralizing Antibodies Against Tick-Borne Encephalitis Virus. In Saravanan Thangamani. Tick-Borne Viruses : Methods and Protocols. 1. vyd. New York: Humana Press Inc., 2025, s. 125-136. Methods in Molecular Biology. ISBN 978-1-0716-4586-4. Dostupné z: https://doi.org/10.1007/978-1-0716-4587-1_14.

@inbook{2551117,
   author = {Eyer, Luděk and Svoboda, Pavel and Palus, Martin and Salát, Jiří and Růžek, Daniel},
   address = {New York},
   booktitle = {Tick-Borne Viruses : Methods and Protocols},
   doi = {https://doi.org/10.1007/978-1-0716-4587-1_14},
   edition = {1},
   editor = {Saravanan Thangamani},
   keywords = {Antivirals; Immunofluorescence; Monoclonal antibodies; Mouse infection; Neutralization test; Plaque assay; Tick-borne encephalitis virus},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {New York},
   isbn = {978-1-0716-4586-4},
   pages = {125-136},
   publisher = {Humana Press Inc.},
   title = {Methods for Evaluation of Antivirals and Neutralizing Antibodies Against Tick-Borne Encephalitis Virus},
   url = {https://link.springer.com/protocol/10.1007/978-1-0716-4587-1_14},
   year = {2025}
}

TY  - CHAP
ID  - 2551117
AU  - Eyer, Luděk - Svoboda, Pavel - Palus, Martin - Salát, Jiří - Růžek, Daniel
PY  - 2025
TI  - Methods for Evaluation of Antivirals and Neutralizing Antibodies Against Tick-Borne Encephalitis Virus
VL  - Methods in Molecular Biology
PB  - Humana Press Inc.
CY  - New York
SN  - 9781071645864
KW  - Antivirals
KW  - Immunofluorescence
KW  - Monoclonal antibodies
KW  - Mouse infection
KW  - Neutralization test
KW  - Plaque assay
KW  - Tick-borne encephalitis virus
UR  - https://link.springer.com/protocol/10.1007/978-1-0716-4587-1_14
N2  - Tick-borne encephalitis (TBE) is a potentially fatal neuroinfection of humans caused by the TBE virus. There is no specific therapy for TBE, as treatment is only supportive. Therefore, efforts to develop an effective specific therapy for TBE are warranted. Small molecule inhibitors and monoclonal antibodies are under intense investigation as potential therapeutics to combat TBE in humans. Here we describe the basic methods that can be used to test small molecule antivirals or monoclonal antibodies against TBEV. These include in vitro methods such as plaque assays, neutralizing assays, immunofluorescence staining of viral antigen, and cell-based antiviral assays, as well as in vivo assays in mouse model of TBE.
ER  -

EYER, Luděk; Pavel SVOBODA; Martin PALUS; Jiří SALÁT a Daniel RŮŽEK. Methods for Evaluation of Antivirals and Neutralizing Antibodies Against Tick-Borne Encephalitis Virus. In Saravanan Thangamani. \textit{Tick-Borne Viruses : Methods and Protocols}. 1. vyd. New York: Humana Press Inc., 2025, s.~125-136. Methods in Molecular Biology. ISBN~978-1-0716-4586-4. Dostupné z: https://doi.org/10.1007/978-1-0716-4587-1\_{}14.

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