Exploring Tick-Borne Encephalitis Virus–Host Cell Interactions
Using Electron Tomography: Methodologies and Protocols

C 2025

Exploring Tick-Borne Encephalitis Virus–Host Cell Interactions Using Electron Tomography: Methodologies and Protocols

VANCOVÁ, Marie; Tomáš BÍLÝ; Martin PALUS a Daniel RŮŽEK

Základní údaje

Originální název

Exploring Tick-Borne Encephalitis Virus–Host Cell Interactions Using Electron Tomography: Methodologies and Protocols

Autoři

VANCOVÁ, Marie; Tomáš BÍLÝ; Martin PALUS a Daniel RŮŽEK

Vydání

1. vyd. New York, Tick-Borne Viruses : Methods and Protocols, od s. 137-150, 14 s. Methods in Molecular Biology, 2025

Nakladatel

Humana Press Inc.

Další údaje

Jazyk

angličtina

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Obor

10607 Virology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/25:00143627

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-1-0716-4586-4

DOI

https://doi.org/10.1007/978-1-0716-4587-1_15

EID Scopus

2-s2.0-105008745176

Klíčová slova anglicky

Tick-borne encephalitis virus; Electron tomography; Transmission electron microscopy

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 2. 2026 14:58, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Electron microscopy stands as a cornerstone in unraveling the intricate dynamics of viral infections, with its high-resolution capabilities offering invaluable insights into the interactions between viruses and the infected cells. Here, we present a comprehensive methodology designed to explore the three-dimensional interactions specifically between tick-borne encephalitis virus (TBEV) and host cells. This approach allows to study all stages of viral lifecycle, including replication, budding, maturation, and host cell defense mechanisms. The methodology encompasses a range of techniques, commencing with sample preparation using high-pressure freezing, followed by freeze substitution, epoxy embedding, and ultrathin sectioning. Subsequently, we employ electron tomography in conjunction with image processing and analysis techniques to unravel the intricate nuances of TBEV–host cell interactions.

Návaznosti

90250, velká výzkumná infrastruktura

Název: Czech-BioImaging III

Citovat

VANCOVÁ, Marie; Tomáš BÍLÝ; Martin PALUS a Daniel RŮŽEK. Exploring Tick-Borne Encephalitis Virus–Host Cell Interactions Using Electron Tomography: Methodologies and Protocols. In Saravanan Thangamani. Tick-Borne Viruses : Methods and Protocols. 1. vyd. New York: Humana Press Inc., 2025, s. 137-150. Methods in Molecular Biology. ISBN 978-1-0716-4586-4. Dostupné z: https://doi.org/10.1007/978-1-0716-4587-1_15.

@inbook{2551119,
   author = {Vancová, Marie and Bílý, Tomáš and Palus, Martin and Růžek, Daniel},
   address = {New York},
   booktitle = {Tick-Borne Viruses : Methods and Protocols},
   doi = {https://doi.org/10.1007/978-1-0716-4587-1_15},
   edition = {1},
   editor = {Saravanan Thangamani},
   keywords = {Tick-borne encephalitis virus; Electron tomography; Transmission electron microscopy},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {New York},
   isbn = {978-1-0716-4586-4},
   pages = {137-150},
   publisher = {Humana Press Inc.},
   title = {Exploring Tick-Borne Encephalitis Virus–Host Cell Interactions Using Electron Tomography: Methodologies and Protocols},
   url = {https://link.springer.com/protocol/10.1007/978-1-0716-4587-1_15},
   year = {2025}
}

TY  - CHAP
ID  - 2551119
AU  - Vancová, Marie - Bílý, Tomáš - Palus, Martin - Růžek, Daniel
PY  - 2025
TI  - Exploring Tick-Borne Encephalitis Virus–Host Cell Interactions Using Electron Tomography: Methodologies and Protocols
VL  - Methods in Molecular Biology
PB  - Humana Press Inc.
CY  - New York
SN  - 9781071645864
KW  - Tick-borne encephalitis virus
KW  - Electron tomography
KW  - Transmission electron microscopy
UR  - https://link.springer.com/protocol/10.1007/978-1-0716-4587-1_15
N2  - Electron microscopy stands as a cornerstone in unraveling the intricate dynamics of viral infections, with its high-resolution capabilities offering invaluable insights into the interactions between viruses and the infected cells. Here, we present a comprehensive methodology designed to explore the three-dimensional interactions specifically between tick-borne encephalitis virus (TBEV) and host cells. This approach allows to study all stages of viral lifecycle, including replication, budding, maturation, and host cell defense mechanisms. The methodology encompasses a range of techniques, commencing with sample preparation using high-pressure freezing, followed by freeze substitution, epoxy embedding, and ultrathin sectioning. Subsequently, we employ electron tomography in conjunction with image processing and analysis techniques to unravel the intricate nuances of TBEV–host cell interactions.
ER  -

VANCOVÁ, Marie; Tomáš BÍLÝ; Martin PALUS a Daniel RŮŽEK. Exploring Tick-Borne Encephalitis Virus–Host Cell Interactions Using Electron Tomography: Methodologies and Protocols. In Saravanan Thangamani. \textit{Tick-Borne Viruses : Methods and Protocols}. 1. vyd. New York: Humana Press Inc., 2025, s.~137-150. Methods in Molecular Biology. ISBN~978-1-0716-4586-4. Dostupné z: https://doi.org/10.1007/978-1-0716-4587-1\_{}15.

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