Cost-effective conversion of BLUESTAR® forensic tablets to the
green region of the emission spectrum

J 2026

Cost-effective conversion of BLUESTAR® forensic tablets to the green region of the emission spectrum

MARTIKAN, Jakub; Hynek MARTIKAN a Petr TÁBORSKÝ

Základní údaje

Originální název

Cost-effective conversion of BLUESTAR® forensic tablets to the green region of the emission spectrum

Autoři

MARTIKAN, Jakub; Hynek MARTIKAN a Petr TÁBORSKÝ

Vydání

MICROCHEMICAL JOURNAL, AMSTERDAM, ELSEVIER, 2026, 0026-265X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.100 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

BLUESTAR (R); Chemiluminescence; CRET (chemiluminescence resonance energy transfer); Fluorescein; Luminol

Štítky

14313010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 2. 2026 10:46, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

In this study, we present an evaluation of a chemiluminescence resonance energy transfer (CRET) system based on the commercial Bluestar (R) formulation, aimed at enhancing the detection of trace bloodstains. In the CRET mechanism, the excited-state 3-aminophthalate, generated during the luminol chemiluminescence reaction, transfers its energy non-radiatively to ground-state fluorescein, which subsequently emits in the green spectral region. Fluorescein was identified as the most effective acceptor owing to its high fluorescence quantum yield and low cost. Compared with conventional luminol emission, the Bluestar (R)-fluorescein system exhibited a pronounced spectral shift from blue (similar to 447 nm) to green (similar to 530 nm), resulting in enhanced visual contrast and improved detectability. Quantitatively, camera-based analysis demonstrated up to a 257 % increase in apparent signal intensity, whereas photomultiplier tube (PMT) measurements showed a more moderate enhancement, underlining the critical influence of detector spectral sensitivity. Importantly, the green emission region is more sensitively perceived by the human eye and digital cameras, which translated into significantly lower detection limits for synthetic bloodstains. This work highlights not only the potential of CRET for improving forensic chemiluminescence assays but also emphasizes the practical role of detector characteristics in determining system sensitivity.

Návaznosti

MUNI/A/1691/2024, interní kód MU

Název: Vývoj metod a instrumentace pro chemickou analýzu a studium přírodních a syntetických materiálů včetně biologicky významných látek 2025

Investor: Masarykova univerzita, Vývoj metod a instrumentace pro chemickou analýzu a studium přírodních a syntetických materiálů včetně biologicky významných látek 2025

Citovat

MARTIKAN, Jakub; Hynek MARTIKAN a Petr TÁBORSKÝ. Cost-effective conversion of BLUESTAR® forensic tablets to the green region of the emission spectrum. MICROCHEMICAL JOURNAL. AMSTERDAM: ELSEVIER, 2026, roč. 220, JAN, s. 1-12. ISSN 0026-265X. Dostupné z: https://doi.org/10.1016/j.microc.2025.116104.

@article{2553760,
   author = {Martikan, Jakub and Martikan, Hynek and Táborský, Petr},
   article_location = {AMSTERDAM},
   article_number = {JAN},
   doi = {https://doi.org/10.1016/j.microc.2025.116104},
   keywords = {BLUESTAR (R); Chemiluminescence; CRET (chemiluminescence resonance energy transfer); Fluorescein; Luminol},
   language = {eng},
   issn = {0026-265X},
   journal = {MICROCHEMICAL JOURNAL},
   title = {Cost-effective conversion of BLUESTAR® forensic tablets to the green region of the emission spectrum},
   url = {https://www.sciencedirect.com/science/article/pii/S0026265X25034538?via%3Dihub},
   volume = {220},
   year = {2026}
}

TY  - JOUR
ID  - 2553760
AU  - Martikan, Jakub - Martikan, Hynek - Táborský, Petr
PY  - 2026
TI  - Cost-effective conversion of BLUESTAR® forensic tablets to the green region of the emission spectrum
JF  - MICROCHEMICAL JOURNAL
VL  - 220
IS  - JAN
SP  - 1-12
EP  - 1-12
PB  - ELSEVIER
SN  - 0026265X
KW  - BLUESTAR (R)
KW  - Chemiluminescence
KW  - CRET (chemiluminescence resonance energy transfer)
KW  - Fluorescein
KW  - Luminol
UR  - https://www.sciencedirect.com/science/article/pii/S0026265X25034538?via%3Dihub
N2  - In this study, we present an evaluation of a chemiluminescence resonance energy transfer (CRET) system based on the commercial Bluestar (R) formulation, aimed at enhancing the detection of trace bloodstains. In the CRET mechanism, the excited-state 3-aminophthalate, generated during the luminol chemiluminescence reaction, transfers its energy non-radiatively to ground-state fluorescein, which subsequently emits in the green spectral region. Fluorescein was identified as the most effective acceptor owing to its high fluorescence quantum yield and low cost. Compared with conventional luminol emission, the Bluestar (R)-fluorescein system exhibited a pronounced spectral shift from blue (similar to 447 nm) to green (similar to 530 nm), resulting in enhanced visual contrast and improved detectability. Quantitatively, camera-based analysis demonstrated up to a 257 % increase in apparent signal intensity, whereas photomultiplier tube (PMT) measurements showed a more moderate enhancement, underlining the critical influence of detector spectral sensitivity. Importantly, the green emission region is more sensitively perceived by the human eye and digital cameras, which translated into significantly lower detection limits for synthetic bloodstains. This work highlights not only the potential of CRET for improving forensic chemiluminescence assays but also emphasizes the practical role of detector characteristics in determining system sensitivity.
ER  -

MARTIKAN, Jakub; Hynek MARTIKAN a Petr TÁBORSKÝ. Cost-effective conversion of BLUESTAR® forensic tablets to the green region of the emission spectrum. \textit{MICROCHEMICAL JOURNAL}. AMSTERDAM: ELSEVIER, 2026, roč.~220, JAN, s.~1-12. ISSN~0026-265X. Dostupné z: https://doi.org/10.1016/j.microc.2025.116104.

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