Identification of Plant Phenolics from Paulownia tomentosa and
Morus alba as Novel PPARγ Partial Agonists and Hypoglycemic
Agents

J 2025

Identification of Plant Phenolics from Paulownia tomentosa and Morus alba as Novel PPARγ Partial Agonists and Hypoglycemic Agents

TREML, Jakub; Jiří VÁCLAVÍK; Lenka MOLČANOVÁ; Marie ČULENOVÁ; Scarlet HUMMELBRUNNER et al.

Základní údaje

Originální název

Identification of Plant Phenolics from Paulownia tomentosa and Morus alba as Novel PPARγ Partial Agonists and Hypoglycemic Agents

Autoři

TREML, Jakub; Jiří VÁCLAVÍK; Lenka MOLČANOVÁ; Marie ČULENOVÁ; Scarlet HUMMELBRUNNER; Cathrina NEUHAUSER; Verena M. DIRSCH; Julian WEGHUBER a Karel ŠMEJKAL

Vydání

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, WASHINGTON, AMER CHEMICAL SOC, 2025, 0021-8561

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.200 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Farmaceutická fakulta

Klíčová slova anglicky

diabetes mellitus; hypoglycemic; natural products; plant phenolics; PPAR gamma

Štítky

rivok, ÚMF, ÚPL

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 3. 2026 17:00, Mgr. Irena Doubková

Anotace

V originále

The aim of our study was to determine the PPAR gamma agonism and hypoglycemic activity of natural phenolics isolated from Paulownia tomentosa and Morus alba. We started with a molecular docking preselection, followed by in vitro cell culture assays, such as PPAR gamma luciferase reporter gene assay and PPAR gamma protein expression by Western blot analysis. The ability of the selected compounds to induce GLUT4 translocation in cell culture and lower blood glucose levels in chicken embryos was also determined. Among the thirty-six plant phenolic compounds, moracin M showed the highest hypoglycemic effect in an in ovo experiment (7.33 +/- 2.37%), followed by mulberrofuran Y (3.84 +/- 1.34%) and diplacone (3.69 +/- 1.37%). Neither moracin M nor mulberrofuran Y showed a clear effect on the enhancement of GLUT4 translocation or agonism on PPAR gamma, while diplacone succeeded in both (3.62 +/- 0.16% and 2.4-fold +/- 0.2, respectively). Thus, we believe that the compounds moracin M, mulberrofuran Y, and diplacone are suitable for further experiments to elucidate their mechanisms of action.

Návaznosti

GA23-04655S, projekt VaV

Název: Role prenylace a glykosylace v protizánětlivé aktivitě a metabolismu přírodních fenolových látek

Investor: Grantová agentura ČR, Role prenylace a glykosylace v protizánětlivé aktivitě a metabolismu přírodních fenolových látek

MUNI/A/1654/2020, interní kód MU

Název: Testování biologické aktivity vybraných přírodních látek in vitro

Investor: Masarykova univerzita, Testování biologické aktivity vybraných přírodních látek in vitro

Citovat

TREML, Jakub; Jiří VÁCLAVÍK; Lenka MOLČANOVÁ; Marie ČULENOVÁ; Scarlet HUMMELBRUNNER; Cathrina NEUHAUSER; Verena M. DIRSCH; Julian WEGHUBER a Karel ŠMEJKAL. Identification of Plant Phenolics from Paulownia tomentosa and Morus alba as Novel PPARγ Partial Agonists and Hypoglycemic Agents. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. WASHINGTON: AMER CHEMICAL SOC, 2025, roč. 73, č. 22, s. 13960-13972. ISSN 0021-8561. Dostupné z: https://doi.org/10.1021/acs.jafc.4c11398.

@article{2554505,
   author = {Treml, Jakub and Václavík, Jiří and Molčanová, Lenka and Čulenová, Marie and Hummelbrunner, Scarlet and Neuhauser, Cathrina and Dirsch, Verena M. and Weghuber, Julian and Šmejkal, Karel},
   article_location = {WASHINGTON},
   article_number = {22},
   doi = {https://doi.org/10.1021/acs.jafc.4c11398},
   keywords = {diabetes mellitus; hypoglycemic; natural products; plant phenolics; PPAR gamma},
   language = {eng},
   issn = {0021-8561},
   journal = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
   title = {Identification of Plant Phenolics from Paulownia tomentosa and Morus alba as Novel PPARγ Partial Agonists and Hypoglycemic Agents},
   url = {https://pubs.acs.org/doi/10.1021/acs.jafc.4c11398?src=getftr&utm_source=clarivate&getft_integrator=clarivate},
   volume = {73},
   year = {2025}
}

TY  - JOUR
ID  - 2554505
AU  - Treml, Jakub - Václavík, Jiří - Molčanová, Lenka - Čulenová, Marie - Hummelbrunner, Scarlet - Neuhauser, Cathrina - Dirsch, Verena M. - Weghuber, Julian - Šmejkal, Karel
PY  - 2025
TI  - Identification of Plant Phenolics from Paulownia tomentosa and Morus alba as Novel PPARγ Partial Agonists and Hypoglycemic Agents
JF  - JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
VL  - 73
IS  - 22
SP  - 13960-13972
EP  - 13960-13972
PB  - AMER CHEMICAL SOC
SN  - 00218561
KW  - diabetes mellitus
KW  - hypoglycemic
KW  - natural products
KW  - plant phenolics
KW  - PPAR gamma
UR  - https://pubs.acs.org/doi/10.1021/acs.jafc.4c11398?src=getftr&utm_source=clarivate&getft_integrator=clarivate
N2  - The aim of our study was to determine the PPAR gamma agonism and hypoglycemic activity of natural phenolics isolated from Paulownia tomentosa and Morus alba. We started with a molecular docking preselection, followed by in vitro cell culture assays, such as PPAR gamma luciferase reporter gene assay and PPAR gamma protein expression by Western blot analysis. The ability of the selected compounds to induce GLUT4 translocation in cell culture and lower blood glucose levels in chicken embryos was also determined. Among the thirty-six plant phenolic compounds, moracin M showed the highest hypoglycemic effect in an in ovo experiment (7.33 +/- 2.37%), followed by mulberrofuran Y (3.84 +/- 1.34%) and diplacone (3.69 +/- 1.37%). Neither moracin M nor mulberrofuran Y showed a clear effect on the enhancement of GLUT4 translocation or agonism on PPAR gamma, while diplacone succeeded in both (3.62 +/- 0.16% and 2.4-fold +/- 0.2, respectively). Thus, we believe that the compounds moracin M, mulberrofuran Y, and diplacone are suitable for further experiments to elucidate their mechanisms of action.
ER  -

TREML, Jakub; Jiří VÁCLAVÍK; Lenka MOLČANOVÁ; Marie ČULENOVÁ; Scarlet HUMMELBRUNNER; Cathrina NEUHAUSER; Verena M. DIRSCH; Julian WEGHUBER a Karel ŠMEJKAL. Identification of Plant Phenolics from Paulownia tomentosa and Morus alba as Novel PPARγ Partial Agonists and Hypoglycemic Agents. \textit{JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}. WASHINGTON: AMER CHEMICAL SOC, 2025, roč.~73, č.~22, s.~13960-13972. ISSN~0021-8561. Dostupné z: https://doi.org/10.1021/acs.jafc.4c11398.

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