Conjugates of photon-upconversion nanoparticles with antibodies
for immunochemical detection of tumor biomarkers

a 2024

Conjugates of photon-upconversion nanoparticles with antibodies for immunochemical detection of tumor biomarkers

ŠPAČEK, Pavel; Ekaterina MAKHNEVA; Antonín HLAVÁČEK; Julie WEISOVÁ; Hans-Heiner GORRIS et al.

Základní údaje

Originální název

Conjugates of photon-upconversion nanoparticles with antibodies for immunochemical detection of tumor biomarkers

Autoři

ŠPAČEK, Pavel; Ekaterina MAKHNEVA; Antonín HLAVÁČEK; Julie WEISOVÁ; Hans-Heiner GORRIS; Petr SKLÁDAL a Zdeněk FARKA

Vydání

NANOCON 2024, 2024

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-88365-20-4

Klíčová slova anglicky

photon-upconversion nanoparticle; immunoassay; massively parallel spectroscopy; bioconjugation; biomarker; prostate-specific antigen; protein p53

Příznaky

Mezinárodní význam

Změněno: 3. 3. 2026 19:52, Mgr. Pavel Špaček

Anotace

V originále

The diagnosis of numerous illnesses relies on the sensitive detection of clinical biomarkers. It is of the utmost importance especially in the case of tumor diseases, allowing for early-stage cancer diagnosis and monitoring of treatment response. Due to the high specificity provided by antibodies, various kinds of immunochemical assays are used for this purpose. However, conventional immunoassays often provide insufficient sensitivity for the detection of low-abundance biomarkers. A common strategy to enhance immunoassay sensitivity involves replacing conventional labels with nanoparticles. Photon-upconversion nanoparticles (UCNPs) stand out among the most promising options. These lanthanide-doped nanocrystals convert near-infrared radiation into visible light, significantly reducing the optical background. Moreover, their emission spectra can be tuned by altering the dopant ions. The heterogeneous immunoassay format is typically predominant due to its high sensitivity and specificity. However, it requires immobilization and washing steps, leading to a prolonged procedure. Conversely, even though homogeneous immunoassays omit these time-consuming steps, they come at the cost of reduced specificity and sensitivity. To overcome these drawbacks, we have developed a novel artificial intelligence-aided homogeneous immunoassay format based on massively parallel spectroscopy (MPS). This single-molecule method utilizes two different UCNP-antibody labels binding to analyte molecules, detecting only sandwich immunocomplexes containing the analyte molecule and both labels with different doping. We have successfully employed this method in immunoassays for tumor biomarkers prostate-specific antigen and protein p53, proving its high potential. MPS promises to become a fast and high-throughput method for the detection of biomarkers.

Citovat

ŠPAČEK, Pavel; Ekaterina MAKHNEVA; Antonín HLAVÁČEK; Julie WEISOVÁ; Hans-Heiner GORRIS; Petr SKLÁDAL a Zdeněk FARKA. Conjugates of photon-upconversion nanoparticles with antibodies for immunochemical detection of tumor biomarkers. In NANOCON 2024. 2024. ISBN 978-80-88365-20-4.

@proceedings{2556277,
   author = {Špaček, Pavel and Makhneva, Ekaterina and Hlaváček, Antonín and Weisová, Julie and Gorris, HansandHeiner and Skládal, Petr and Farka, Zdeněk},
   booktitle = {NANOCON 2024},
   keywords = {photon-upconversion nanoparticle; immunoassay; massively parallel spectroscopy; bioconjugation; biomarker; prostate-specific antigen; protein p53},
   language = {eng},
   isbn = {978-80-88365-20-4},
   title = {Conjugates of photon-upconversion nanoparticles with antibodies for immunochemical detection of tumor biomarkers},
   url = {https://nanocon2024.tanger.cz/cz/sbornik-abstraktu/},
   year = {2024}
}

TY  - CONF
ID  - 2556277
AU  - Špaček, Pavel - Makhneva, Ekaterina - Hlaváček, Antonín - Weisová, Julie - Gorris, Hans-Heiner - Skládal, Petr - Farka, Zdeněk
PY  - 2024
TI  - Conjugates of photon-upconversion nanoparticles with antibodies for immunochemical detection of tumor biomarkers
SN  - 9788088365204
KW  - photon-upconversion nanoparticle
KW  - immunoassay
KW  - massively parallel spectroscopy
KW  - bioconjugation
KW  - biomarker
KW  - prostate-specific antigen
KW  - protein p53
UR  - https://nanocon2024.tanger.cz/cz/sbornik-abstraktu/
N2  - The diagnosis of numerous illnesses relies on the sensitive detection of clinical biomarkers. It is of the utmost importance especially in the case of tumor diseases, allowing for early-stage cancer diagnosis and monitoring of treatment response. Due to the high specificity provided by antibodies, various kinds of immunochemical assays are used for this purpose. However, conventional immunoassays often provide insufficient sensitivity for the detection of low-abundance biomarkers. A common strategy to enhance immunoassay sensitivity involves replacing conventional labels with nanoparticles. Photon-upconversion nanoparticles (UCNPs) stand out among the most promising options. These lanthanide-doped nanocrystals convert near-infrared radiation into visible light, significantly reducing the optical background. Moreover, their emission spectra can be tuned by altering the dopant ions. The heterogeneous immunoassay format is typically predominant due to its high sensitivity and specificity. However, it requires immobilization and washing steps, leading to a prolonged procedure. Conversely, even though homogeneous immunoassays omit these time-consuming steps, they come at the cost of reduced specificity and sensitivity. To overcome these drawbacks, we have developed a novel artificial intelligence-aided homogeneous immunoassay format based on massively parallel spectroscopy (MPS). This single-molecule method utilizes two different UCNP-antibody labels binding to analyte molecules, detecting only sandwich immunocomplexes containing the analyte molecule and both labels with different doping. We have successfully employed this method in immunoassays for tumor biomarkers prostate-specific antigen and protein p53, proving its high potential. MPS promises to become a fast and high-throughput method for the detection of biomarkers.
ER  -

Přehled o publikaci