Microbiological findings and antimicrobial resistance dynamics
in pathogens isolated from patients with toxic epidermal
necrolysis: a single center experience

J 2025

Microbiological findings and antimicrobial resistance dynamics in pathogens isolated from patients with toxic epidermal necrolysis: a single center experience

HOLOUBEK, Jakub; Dominika MATYSKOVÁ; M. HANSLIANOVA; Michaela CVANOVÁ; Břetislav LIPOVÝ et al.

Základní údaje

Originální název

Microbiological findings and antimicrobial resistance dynamics in pathogens isolated from patients with toxic epidermal necrolysis: a single center experience

Autoři

HOLOUBEK, Jakub; Dominika MATYSKOVÁ; M. HANSLIANOVA; Michaela CVANOVÁ a Břetislav LIPOVÝ

Vydání

Čs. epidemiologie, mikrobiologie, imunologie, Praha, ČLS JEP, 2025, 1210-7913

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30102 Immunology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 0.500 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

toxic epidermal necrolysis; infectious complications; antimicrobial resistance

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 2. 2026 11:46, Mgr. Tereza Miškechová

Anotace

V originále

Introduction: Toxic epidermal necrolysis (TEN) is one of the most life-threatening skin exfoliative disorders. The disease is frequently misdiagnosed or diagnosed with a long delay. This, together with the unique pathology, leads to a high mortality of affected patients. The high risk of infections associated with extensive loss of skin and impaired function of the immune system is one of the most common complications in TEN patients. Methods: All patients treated at our clinic with a diagnosis of TEN between January 1, 1996, and December 31, 2020, were included in this study. Basic epidemiological parameters, the incidence of infectious complications in individual compartments, clinical laboratory and microbiological parameters, and antimicrobial resistance of pathogens isolated during the hospital stay were collected. Results: In total, 498 potentially pathogenic microorganisms (PPMs) were isolated in our patient group, i.e., on average more than 35 isolated PPMs per patient. Of these, 155 pathogens fell into the gram-positive spectrum (31.1%), and 331 into the gram-negative spectrum (66.5%). The effect of corticosteroid therapy on overall survival is also an interesting indicator. In patients with multifocal infection, the maximum corticosteroid dose was 965 mg of methylprednisolone, while in patients without multifocal infection, the maximum dose was 2.333 mg methylprednisolone (p = 0.032). Last but not least, we mapped the most frequently occurring pathogens, dominated by gram-negative bacteria, in particular Klebsiella sp. and Enterobacter sp. We also described the presence of antibiotic resistance in epidemiologically important pathogens in TEN patients with infectious complications. Conclusion: We successfully identified basic epidemiological parameters in a cohort of patients treated with TEN along with the most frequent representatives of infectious complications. We were able to identify risk factors for the development of infectious complications and their impact on further complications of treatment as well as patient mortality.

Citovat

HOLOUBEK, Jakub; Dominika MATYSKOVÁ; M. HANSLIANOVA; Michaela CVANOVÁ a Břetislav LIPOVÝ. Microbiological findings and antimicrobial resistance dynamics in pathogens isolated from patients with toxic epidermal necrolysis: a single center experience. Čs. epidemiologie, mikrobiologie, imunologie. Praha: ČLS JEP, 2025, roč. 74, č. 4, s. 205-215. ISSN 1210-7913. Dostupné z: https://doi.org/10.61568/emi/11-6600/20251001/141813.

@article{2556421,
   author = {Holoubek, Jakub and Matysková, Dominika and Hanslianova, M. and Cvanová, Michaela and Lipový, Břetislav},
   article_location = {Praha},
   article_number = {4},
   doi = {https://doi.org/10.61568/emi/11-6600/20251001/141813},
   keywords = {toxic epidermal necrolysis; infectious complications; antimicrobial resistance},
   language = {eng},
   issn = {1210-7913},
   journal = {Čs. epidemiologie, mikrobiologie, imunologie},
   title = {Microbiological findings and antimicrobial resistance dynamics in pathogens isolated from patients with toxic epidermal necrolysis: a single center experience},
   url = {https://www.prolekare.cz/casopisy/epidemiologie/2025-4-11/microbiological-findings-and-antimicrobial-resistance-dynamics-in-pathogens-isolated-from-patients-with-toxic-epidermal-necrolysis-a-single-center-experience-141813},
   volume = {74},
   year = {2025}
}

TY  - JOUR
ID  - 2556421
AU  - Holoubek, Jakub - Matysková, Dominika - Hanslianova, M. - Cvanová, Michaela - Lipový, Břetislav
PY  - 2025
TI  - Microbiological findings and antimicrobial resistance dynamics in pathogens isolated from patients with toxic epidermal necrolysis: a single center experience
JF  - Čs. epidemiologie, mikrobiologie, imunologie
VL  - 74
IS  - 4
SP  - 205-215
EP  - 205-215
PB  - ČLS JEP
SN  - 12107913
KW  - toxic epidermal necrolysis
KW  - infectious complications
KW  - antimicrobial resistance
UR  - https://www.prolekare.cz/casopisy/epidemiologie/2025-4-11/microbiological-findings-and-antimicrobial-resistance-dynamics-in-pathogens-isolated-from-patients-with-toxic-epidermal-necrolysis-a-single-center-experience-141813
N2  - Introduction: Toxic epidermal necrolysis (TEN) is one of the most life-threatening skin exfoliative disorders. The disease is frequently misdiagnosed or diagnosed with a long delay. This, together with the unique pathology, leads to a high mortality of affected patients. The high risk of infections associated with extensive loss of skin and impaired function of the immune system is one of the most common complications in TEN patients. Methods: All patients treated at our clinic with a diagnosis of TEN between January 1, 1996, and December 31, 2020, were included in this study. Basic epidemiological parameters, the incidence of infectious complications in individual compartments, clinical laboratory and microbiological parameters, and antimicrobial resistance of pathogens isolated during the hospital stay were collected. Results: In total, 498 potentially pathogenic microorganisms (PPMs) were isolated in our patient group, i.e., on average more than 35 isolated PPMs per patient. Of these, 155 pathogens fell into the gram-positive spectrum (31.1%), and 331 into the gram-negative spectrum (66.5%). The effect of corticosteroid therapy on overall survival is also an interesting indicator. In patients with multifocal infection, the maximum corticosteroid dose was 965 mg of methylprednisolone, while in patients without multifocal infection, the maximum dose was 2.333 mg methylprednisolone (p = 0.032). Last but not least, we mapped the most frequently occurring pathogens, dominated by gram-negative bacteria, in particular Klebsiella sp. and Enterobacter sp. We also described the presence of antibiotic resistance in epidemiologically important pathogens in TEN patients with infectious complications. Conclusion: We successfully identified basic epidemiological parameters in a cohort of patients treated with TEN along with the most frequent representatives of infectious complications. We were able to identify risk factors for the development of infectious complications and their impact on further complications of treatment as well as patient mortality.
ER  -

HOLOUBEK, Jakub; Dominika MATYSKOVÁ; M. HANSLIANOVA; Michaela CVANOVÁ a Břetislav LIPOVÝ. Microbiological findings and antimicrobial resistance dynamics in pathogens isolated from patients with toxic epidermal necrolysis: a single center experience. \textit{Čs. epidemiologie, mikrobiologie, imunologie}. Praha: ČLS JEP, 2025, roč.~74, č.~4, s.~205-215. ISSN~1210-7913. Dostupné z: https://doi.org/10.61568/emi/11-6600/20251001/141813.

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