Toward needle-free lamotrigine monitoring: Proof-of-concept for
saliva and dried saliva spot analysis by LC-MS/MS - a short
communication

J 2026

Toward needle-free lamotrigine monitoring: Proof-of-concept for saliva and dried saliva spot analysis by LC-MS/MS - a short communication

ĎURČOVÁ, Viktória; Marta PELCOVÁ; Pavel ŠMAK; Ondřej STRÝČEK; Jana GREGOROVÁ et al.

Základní údaje

Originální název

Toward needle-free lamotrigine monitoring: Proof-of-concept for saliva and dried saliva spot analysis by LC-MS/MS - a short communication

Autoři

ĎURČOVÁ, Viktória; Marta PELCOVÁ; Pavel ŠMAK; Ondřej STRÝČEK; Jana GREGOROVÁ ; Ondřej PEŠ; Zdeněk GLATZ; Pavel SISTIK a Jan JUŘICA

Vydání

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Amsterdam, Elsevier, 2026, 1570-0232

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.800 v roce 2024

Označené pro přenos do RIV

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Lamotrigine; Saliva; Anti-seizure medication; Dried saliva spot; Therapeutic drug monitoring

Štítky

14313050, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 2. 2026 09:57, Mgr. Tereza Miškechová

Anotace

V originále

Recent advancements in micro-sampling methods provide non-invasive options for collecting biological samples used in therapeutic drug monitoring (TDM). Lately, alternative matrices such as capillary blood and saliva/oral fluid in the form of dried saliva spots, have attracted considerable interest. These matrices represent a promising approach for TDM, as they could improve accessibility while preserving analytical precision. The study developed and validated an LC-MS method for lamotrigine quantitation in saliva and Dried Saliva Spots (DSS). Samples were prepared via protein precipitation (methanol: acetonitrile: water, 7:2:1) and dilution, followed by separation on a Luna Omega C18 Polar column and detection in ESI+ mode. An isotopically labelled internal standard was employed, ensuring compliance with EMA guidelines. The linearity was demonstrated for lamotrigine concentrations ranging from 0.20 to 25 mg/L in both dried saliva spot and saliva samples. Saliva and DSS samples from patients were used to compare lamotrigine concentrations with plasma levels determined by a hospital-accredited laboratory. Findings demonstrated a strong correlation between evaluated matrices and plasma (R = 0.847 for saliva and plasma, and R = 0.839 for DSS and plasma, both p < 0.0001), underscoring the promise of saliva and DSS as substitutes for TDM. This technique improves patient comfort and accessibility through a non-invasive sampling method while ensuring accuracy. These findings provide a foundation for broader clinical implementation of alternative-matrix TDM, representing a significant step toward patient-centred, accessible pharmacotherapy and enabling improved LTG monitoring in ambulatory and remote settings.

Návaznosti

MUNI/A/1676/2024, interní kód MU

Název: Využití chemických a bioanalytických technik pro zkoumání biochemické podstaty vybraných patologických procesů.

Investor: Masarykova univerzita, Využití chemických a bioanalytických technik pro zkoumání biochemické podstaty vybraných patologických procesů.

MUNI/A/1684/2024, interní kód MU

Název: Podpora biochemického výzkumu v roce 2025

Investor: Masarykova univerzita, Podpora biochemického výzkumu v roce 2025

MUNI/A/1722/2024, interní kód MU

Název: Specifický farmakologický výzkum v oblasti farmakokinetiky, behaviorální neuropsychofarmakologie a personalizované farmakoterapie v onkologii

Investor: Masarykova univerzita, Specifický farmakologický výzkum v oblasti farmakokinetiky, behaviorální neuropsychofarmakologie a personalizované farmakoterapie v onkologii

NU23-08-00229, projekt VaV

Název: Minimálně invazivní metody pro personalizovanou farmakoterapii epilepsie

Investor: Ministerstvo zdravotnictví ČR, Minimálně invazivní metody pro personalizovanou farmakoterapii epilepsie, Podprogram 1 - standardní

Citovat

ĎURČOVÁ, Viktória; Marta PELCOVÁ; Pavel ŠMAK; Ondřej STRÝČEK; Jana GREGOROVÁ; Ondřej PEŠ; Zdeněk GLATZ; Pavel SISTIK a Jan JUŘICA. Toward needle-free lamotrigine monitoring: Proof-of-concept for saliva and dried saliva spot analysis by LC-MS/MS - a short communication. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. Amsterdam: Elsevier, 2026, roč. 1271, March 2026, s. 1-6. ISSN 1570-0232. Dostupné z: https://doi.org/10.1016/j.jchromb.2026.124929.

@article{2556621,
   author = {Ďurčová, Viktória and Pelcová, Marta and Šmak, Pavel and Strýček, Ondřej and Gregorová, Jana and Peš, Ondřej and Glatz, Zdeněk and Sistik, Pavel and Juřica, Jan},
   article_location = {Amsterdam},
   article_number = {March 2026},
   doi = {https://doi.org/10.1016/j.jchromb.2026.124929},
   keywords = {Lamotrigine; Saliva; Anti-seizure medication; Dried saliva spot; Therapeutic drug monitoring},
   language = {eng},
   issn = {1570-0232},
   journal = {Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences},
   title = {Toward needle-free lamotrigine monitoring: Proof-of-concept for saliva and dried saliva spot analysis by LC-MS/MS - a short communication},
   url = {https://www.sciencedirect.com/science/article/pii/S1570023226000188?via%3Dihub},
   volume = {1271},
   year = {2026}
}

TY  - JOUR
ID  - 2556621
AU  - Ďurčová, Viktória - Pelcová, Marta - Šmak, Pavel - Strýček, Ondřej - Gregorová, Jana - Peš, Ondřej - Glatz, Zdeněk - Sistik, Pavel - Juřica, Jan
PY  - 2026
TI  - Toward needle-free lamotrigine monitoring: Proof-of-concept for saliva and dried saliva spot analysis by LC-MS/MS - a short communication
JF  - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
VL  - 1271
IS  - March 2026
SP  - 1-6
EP  - 1-6
PB  - Elsevier
SN  - 15700232
KW  - Lamotrigine
KW  - Saliva
KW  - Anti-seizure medication
KW  - Dried saliva spot
KW  - Therapeutic drug monitoring
UR  - https://www.sciencedirect.com/science/article/pii/S1570023226000188?via%3Dihub
N2  - Recent advancements in micro-sampling methods provide non-invasive options for collecting biological samples used in therapeutic drug monitoring (TDM). Lately, alternative matrices such as capillary blood and saliva/oral fluid in the form of dried saliva spots, have attracted considerable interest. These matrices represent a promising approach for TDM, as they could improve accessibility while preserving analytical precision. The study developed and validated an LC-MS method for lamotrigine quantitation in saliva and Dried Saliva Spots (DSS). Samples were prepared via protein precipitation (methanol: acetonitrile: water, 7:2:1) and dilution, followed by separation on a Luna Omega C18 Polar column and detection in ESI+ mode. An isotopically labelled internal standard was employed, ensuring compliance with EMA guidelines. The linearity was demonstrated for lamotrigine concentrations ranging from 0.20 to 25 mg/L in both dried saliva spot and saliva samples. Saliva and DSS samples from patients were used to compare lamotrigine concentrations with plasma levels determined by a hospital-accredited laboratory. Findings demonstrated a strong correlation between evaluated matrices and plasma (R = 0.847 for saliva and plasma, and R = 0.839 for DSS and plasma, both p < 0.0001), underscoring the promise of saliva and DSS as substitutes for TDM. This technique improves patient comfort and accessibility through a non-invasive sampling method while ensuring accuracy. These findings provide a foundation for broader clinical implementation of alternative-matrix TDM, representing a significant step toward patient-centred, accessible pharmacotherapy and enabling improved LTG monitoring in ambulatory and remote settings.
ER  -

ĎURČOVÁ, Viktória; Marta PELCOVÁ; Pavel ŠMAK; Ondřej STRÝČEK; Jana GREGOROVÁ; Ondřej PEŠ; Zdeněk GLATZ; Pavel SISTIK a Jan JUŘICA. Toward needle-free lamotrigine monitoring: Proof-of-concept for saliva and dried saliva spot analysis by LC-MS/MS - a short communication. \textit{Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences}. Amsterdam: Elsevier, 2026, roč.~1271, March 2026, s.~1-6. ISSN~1570-0232. Dostupné z: https://doi.org/10.1016/j.jchromb.2026.124929.

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