Conjugates of photon-upconversion nanoparticles with antibodies
for immunochemical detection of tumor biomarkers

a 2024

Conjugates of photon-upconversion nanoparticles with antibodies for immunochemical detection of tumor biomarkers

ŠPAČEK, Pavel; Ekaterina MAKHNEVA; Antonín HLAVÁČEK; Julie WEISOVÁ; Hans-Heiner GORRIS et al.

Základní údaje

Originální název

Conjugates of photon-upconversion nanoparticles with antibodies for immunochemical detection of tumor biomarkers

Autoři

ŠPAČEK, Pavel; Ekaterina MAKHNEVA; Antonín HLAVÁČEK; Julie WEISOVÁ; Hans-Heiner GORRIS; Petr SKLÁDAL a Zdeněk FARKA

Vydání

XXIII. Meeting of biochemists and molecular biologists, 2024

Další údaje

Typ výsledku

Konferenční abstrakt

Obor

10406 Analytical chemistry

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-280-0621-1

Klíčová slova anglicky

photon-upconversion nanoparticle; immunoassay; massively parallel spectroscopy; bioconjugation; biomarker; prostate-specific antigen; protein p53

Příznaky

Mezinárodní význam

Změněno: 25. 2. 2026 10:58, Mgr. Pavel Špaček

Anotace

V originále

Sensitive detection of clinical biomarkers is crucial for accurate identification of numerous illnesses. High sensitivity is especially important in the case of tumor diseases, allowing for early-stage cancer diagnosis and assessing treatment response. Due to the high specificity conferred by antibodies, immunochemical assays are often used for this purpose. However, conventional immunoassay labels, such as enzymes and fluorophores, often provide insufficient sensitivity for the detection of low-abundance biomarkers. Therefore, various kinds of nanoparticles have been investigated as labels to enhance immunoassay sensitivity. Photon upconversion nanoparticles (UCNPs) stand out as one of the most promising options. These lanthanide-doped nanocrystals can convert near-infrared radiation into light of a shorter wavelength (anti-Stokes emission), significantly reducing the optical background. Moreover, their luminescent properties can be tuned by altering the composition of dopant ions. The heterogeneous immunoassay format is typically predominant due to its high sensitivity and specificity. However, it requires immobilization and washing steps, leading to a prolonged procedure. In contrast, even though homogeneous immunoassays omit these time-consuming steps, they come at the cost of reduced specificity and sensitivity. To overcome these drawbacks, we have developed a novel homogeneous immunoassay format based on massively parallel spectroscopy (MPS). This single-molecule method utilizes two different UCNP antibody labels with distinct emission spectra binding to the analyte molecule. Only sandwich immunocomplexes containing the analyte molecule and both labels are detected. We successfully applied MPS in assays for prostate specific antigen and protein p53, two important tumor biomarkers. Such an immunoassay format was utilized for the first time, showing a strong potential to become a convenient, high-throughput bioanalytical method.

Citovat

ŠPAČEK, Pavel; Ekaterina MAKHNEVA; Antonín HLAVÁČEK; Julie WEISOVÁ; Hans-Heiner GORRIS; Petr SKLÁDAL a Zdeněk FARKA. Conjugates of photon-upconversion nanoparticles with antibodies for immunochemical detection of tumor biomarkers. In XXIII. Meeting of biochemists and molecular biologists. 2024. ISBN 978-80-280-0621-1.

@proceedings{2556643,
   author = {Špaček, Pavel and Makhneva, Ekaterina and Hlaváček, Antonín and Weisová, Julie and Gorris, HansandHeiner and Skládal, Petr and Farka, Zdeněk},
   booktitle = {XXIII. Meeting of biochemists and molecular biologists},
   keywords = {photon-upconversion nanoparticle; immunoassay; massively parallel spectroscopy; bioconjugation; biomarker; prostate-specific antigen; protein p53},
   isbn = {978-80-280-0621-1},
   title = {Conjugates of photon-upconversion nanoparticles with antibodies for immunochemical detection of tumor biomarkers},
   url = {https://www.muni.cz/vyzkum/publikace/2483637},
   year = {2024}
}

TY  - CONF
ID  - 2556643
AU  - Špaček, Pavel - Makhneva, Ekaterina - Hlaváček, Antonín - Weisová, Julie - Gorris, Hans-Heiner - Skládal, Petr - Farka, Zdeněk
PY  - 2024
TI  - Conjugates of photon-upconversion nanoparticles with antibodies for immunochemical detection of tumor biomarkers
SN  - 9788028006211
KW  - photon-upconversion nanoparticle
KW  - immunoassay
KW  - massively parallel spectroscopy
KW  - bioconjugation
KW  - biomarker
KW  - prostate-specific antigen
KW  - protein p53
UR  - https://www.muni.cz/vyzkum/publikace/2483637
N2  - Sensitive detection of clinical biomarkers is crucial for accurate identification of numerous illnesses. High sensitivity is especially important in the case of tumor diseases, allowing for early-stage cancer diagnosis and assessing treatment response. Due to the high specificity conferred by antibodies, immunochemical assays are often used for this purpose. However, conventional immunoassay labels, such as enzymes and fluorophores, often provide insufficient sensitivity for the detection of low-abundance biomarkers. Therefore, various kinds of nanoparticles have been investigated as labels to enhance immunoassay sensitivity. Photon upconversion nanoparticles (UCNPs) stand out as one of the most promising options. These lanthanide-doped nanocrystals can convert near-infrared radiation into light of a shorter wavelength (anti-Stokes emission), significantly reducing the optical background. Moreover, their luminescent properties can be tuned by altering the composition of dopant ions. The heterogeneous immunoassay format is typically predominant due to its high sensitivity and specificity. However, it requires immobilization and washing steps, leading to a prolonged procedure. In contrast, even though homogeneous immunoassays omit these time-consuming steps, they come at the cost of reduced specificity and sensitivity. To overcome these drawbacks, we have developed a novel homogeneous immunoassay format based on massively parallel spectroscopy (MPS). This single-molecule method utilizes two different UCNP antibody labels with distinct emission spectra binding to the analyte molecule. Only sandwich immunocomplexes containing the analyte molecule and both labels are detected. We successfully applied MPS in assays for prostate specific antigen and protein p53, two important tumor biomarkers. Such an immunoassay format was utilized for the first time, showing a strong potential to become a convenient, high-throughput bioanalytical method.
ER  -

ŠPAČEK, Pavel; Ekaterina MAKHNEVA; Antonín HLAVÁČEK; Julie WEISOVÁ; Hans-Heiner GORRIS; Petr SKLÁDAL a Zdeněk FARKA. Conjugates of photon-upconversion nanoparticles with antibodies for immunochemical detection of tumor biomarkers. In \textit{XXIII. Meeting of biochemists and molecular biologists}. 2024. ISBN~978-80-280-0621-1.

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