Photosystem II supercomplexes lacking light-harvesting antenna
protein LHCB5 and their organization in the thylakoid membrane

J 2025

Photosystem II supercomplexes lacking light-harvesting antenna protein LHCB5 and their organization in the thylakoid membrane

VÁNSKÁ, Tereza; Roman KOUŘIL; Monika OPATÍKOVÁ; Iva ILÍKOVÁ; Rameez ARSHAD et al.

Základní údaje

Originální název

Photosystem II supercomplexes lacking light-harvesting antenna protein LHCB5 and their organization in the thylakoid membrane

Autoři

VÁNSKÁ, Tereza; Roman KOUŘIL; Monika OPATÍKOVÁ; Iva ILÍKOVÁ; Rameez ARSHAD; Pavel ROUDNICKÝ a Petr ILÍK

Vydání

PHYSIOLOGIA PLANTARUM, WILEY-BLACKWELL, 2025, 0031-9317

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.600 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/25:00143807

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

electron-transport; grana membranes; energy-transfer; arabidopsis; cp24; complex; macrostructure; carotenoids

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 3. 2026 09:59, Mgr. Eva Dubská

Anotace

V originále

Light-harvesting protein LHCB5 is one of the three minor antenna proteins (LHCB4-6) that connect the core (C) of photosystem II (PSII) with strongly (S) and moderately (M) bound peripheral trimeric antennae (LHCIIs), forming a dimeric PSII supercomplex known as C2S2M2. Plants lacking LHCB4 and LHCB6 do not form C2S2M2, indicating that these minor antenna proteins are crucial for C(2)S(2)M(2 )assembly. However, studies on antisense asLhcb5 plants suggest this may not apply to LHCB5. Using mild clear-native PAGE (CN-PAGE) and electron microscopy (EM), we separated and structurally characterized the C(2)S(2)M(2 )supercomplex from the Arabidopsis lhcb5 mutant. When compared with wild type (WT), the C(2)S(2)M(2 )supercomplexes in the lhcb5 mutant have slightly different positions of S and M trimers and are generally smaller and present in the thylakoid membrane at higher density. Using CN-PAGE, we did not observe any PSII megacomplexes in the lhcb5 mutant, although they are routinely detected by this method in WT. However, we identified the megacomplexes directly in thylakoid membranes via EM, indicating that the megacomplexes are formed but are too labile to be separated. While in WT, both parallel- and non-parallel-associated PSII supercomplexes can be detected in the thylakoid membrane (Nosek et al., 2017, Plant Journal 89, pp. 104-111), only the parallel-associated PSII supercomplexes were found in the lhcb5 mutant. This finding suggests that the formation of non-parallel-associated PSII supercomplexes depends on the presence of LHCB5. The presence of large PSII supercomplexes and megacomplexes, even though less stable, could explain the WT-like photosynthetic characteristics of the lhcb5 mutant.

Návaznosti

EH22_008/0004581, projekt VaV

Název: Nové poznatky pro plodiny nové generace

90242, velká výzkumná infrastruktura

Název: CIISB III

90254, velká výzkumná infrastruktura

Název: e-INFRA CZ II

Citovat

VÁNSKÁ, Tereza; Roman KOUŘIL; Monika OPATÍKOVÁ; Iva ILÍKOVÁ; Rameez ARSHAD; Pavel ROUDNICKÝ a Petr ILÍK. Photosystem II supercomplexes lacking light-harvesting antenna protein LHCB5 and their organization in the thylakoid membrane. PHYSIOLOGIA PLANTARUM. WILEY-BLACKWELL, 2025, roč. 177, č. 2, s. 1-10. ISSN 0031-9317. Dostupné z: https://doi.org/10.1111/ppl.70167.

@article{2557198,
   author = {Vánská, Tereza and Kouřil, Roman and Opatíková, Monika and Ilíková, Iva and Arshad, Rameez and Roudnický, Pavel and Ilík, Petr},
   article_number = {2},
   doi = {https://doi.org/10.1111/ppl.70167},
   keywords = {electron-transport; grana membranes; energy-transfer; arabidopsis; cp24; complex; macrostructure; carotenoids},
   language = {eng},
   issn = {0031-9317},
   journal = {PHYSIOLOGIA PLANTARUM},
   title = {Photosystem II supercomplexes lacking light-harvesting antenna protein LHCB5 and their organization in the thylakoid membrane},
   url = {https://onlinelibrary.wiley.com/doi/10.1111/ppl.70167},
   volume = {177},
   year = {2025}
}

TY  - JOUR
ID  - 2557198
AU  - Vánská, Tereza - Kouřil, Roman - Opatíková, Monika - Ilíková, Iva - Arshad, Rameez - Roudnický, Pavel - Ilík, Petr
PY  - 2025
TI  - Photosystem II supercomplexes lacking light-harvesting antenna protein LHCB5 and their organization in the thylakoid membrane
JF  - PHYSIOLOGIA PLANTARUM
VL  - 177
IS  - 2
SP  - 1-10
EP  - 1-10
PB  - WILEY-BLACKWELL
SN  - 00319317
KW  - electron-transport
KW  - grana membranes
KW  - energy-transfer
KW  - arabidopsis
KW  - cp24
KW  - complex
KW  - macrostructure
KW  - carotenoids
UR  - https://onlinelibrary.wiley.com/doi/10.1111/ppl.70167
N2  - Light-harvesting protein LHCB5 is one of the three minor antenna proteins (LHCB4-6) that connect the core (C) of photosystem II (PSII) with strongly (S) and moderately (M) bound peripheral trimeric antennae (LHCIIs), forming a dimeric PSII supercomplex known as C2S2M2. Plants lacking LHCB4 and LHCB6 do not form C2S2M2, indicating that these minor antenna proteins are crucial for C(2)S(2)M(2 )assembly. However, studies on antisense asLhcb5 plants suggest this may not apply to LHCB5. Using mild clear-native PAGE (CN-PAGE) and electron microscopy (EM), we separated and structurally characterized the C(2)S(2)M(2 )supercomplex from the Arabidopsis lhcb5 mutant. When compared with wild type (WT), the C(2)S(2)M(2 )supercomplexes in the lhcb5 mutant have slightly different positions of S and M trimers and are generally smaller and present in the thylakoid membrane at higher density. Using CN-PAGE, we did not observe any PSII megacomplexes in the lhcb5 mutant, although they are routinely detected by this method in WT. However, we identified the megacomplexes directly in thylakoid membranes via EM, indicating that the megacomplexes are formed but are too labile to be separated. While in WT, both parallel- and non-parallel-associated PSII supercomplexes can be detected in the thylakoid membrane (Nosek et al., 2017, Plant Journal 89, pp. 104-111), only the parallel-associated PSII supercomplexes were found in the lhcb5 mutant. This finding suggests that the formation of non-parallel-associated PSII supercomplexes depends on the presence of LHCB5. The presence of large PSII supercomplexes and megacomplexes, even though less stable, could explain the WT-like photosynthetic characteristics of the lhcb5 mutant.
ER  -

VÁNSKÁ, Tereza; Roman KOUŘIL; Monika OPATÍKOVÁ; Iva ILÍKOVÁ; Rameez ARSHAD; Pavel ROUDNICKÝ a Petr ILÍK. Photosystem II supercomplexes lacking light-harvesting antenna protein LHCB5 and their organization in the thylakoid membrane. \textit{PHYSIOLOGIA PLANTARUM}. WILEY-BLACKWELL, 2025, roč.~177, č.~2, s.~1-10. ISSN~0031-9317. Dostupné z: https://doi.org/10.1111/ppl.70167.

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