Structural Characterization of Pragia fontium Lipid A by MALDI
Mass Spectrometry

J 2025

Structural Characterization of Pragia fontium Lipid A by MALDI Mass Spectrometry

NARTEY, Linda K; Abanoub MIKHAEL; Hyojik YANG; David ŠMAJS; Robert K ERNST et al.

Základní údaje

Originální název

Structural Characterization of Pragia fontium Lipid A by MALDI Mass Spectrometry

Autoři

NARTEY, Linda K; Abanoub MIKHAEL; Hyojik YANG; David ŠMAJS; Robert K ERNST; Helena PETROSOVA a David R GOODLETT

Vydání

Rapid Communications in Mass Spectrometry, HOBOKEN, Wiley, 2025, 0951-4198

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.700 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/25:00143818

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Lipid A; MALDI-TOF MS; Ozonation; P. fontium

Štítky

14110513, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 3. 2026 10:31, Mgr. Tereza Miškechová

Anotace

V originále

Here, we report the first structural characterization of lipid A from Pragia fontium isolated from well water in South Bohemia, Czech Republic, in 1982, using our Fast Lipid Analysis Technique (FLAT) and its tandem mass spectrometry version, FLATn. P. fontium is a non-pathogenic environmental bacterium within the Enterobacteriaceae family, which is in the same genera as clinically important species Escherichia, Klebsiella, and Salmonella. P. fontium is not known to cause disease; however, studying its lipid A expands our current understanding of the structural diversity of lipid A within the Enterobacteriaceae beyond clinically relevant species and particularly in underexplored environmental isolates. Understanding such variation provides insight into evolutionary adaptations and structural features critical for environmental survival and immune recognition. Our analysis identified the major P. fontium lipid A molecule, distinguishing it from other Enterobacteriaceae. Lipid A is the bioactive component of lipopolysaccharide (LPS), essential for the structural integrity of Gram-negative bacterial outer membranes and a key modulator of host immune responses. Although the lipid A structures of many pathogenic bacteria have been extensively studied, limited information is available on non-pathogenic or environmental isolates, such as P. fontium. These findings provide new insights into the structural biology of this organism and may have implications for its interaction with the environment and host immune responses.

Citovat

NARTEY, Linda K; Abanoub MIKHAEL; Hyojik YANG; David ŠMAJS; Robert K ERNST; Helena PETROSOVA a David R GOODLETT. Structural Characterization of Pragia fontium Lipid A by MALDI Mass Spectrometry. Rapid Communications in Mass Spectrometry. HOBOKEN: Wiley, 2025, roč. 39, č. 18, s. 1-4. ISSN 0951-4198. Dostupné z: https://doi.org/10.1002/rcm.10087.

@article{2557759,
   author = {Nartey, Linda K and Mikhael, Abanoub and Yang, Hyojik and Šmajs, David and Ernst, Robert K and Petrosova, Helena and Goodlett, David R},
   article_location = {HOBOKEN},
   article_number = {18},
   doi = {https://doi.org/10.1002/rcm.10087},
   keywords = {Lipid A; MALDI-TOF MS; Ozonation; P. fontium},
   language = {eng},
   issn = {0951-4198},
   journal = {Rapid Communications in Mass Spectrometry},
   title = {Structural Characterization of Pragia fontium Lipid A by MALDI Mass Spectrometry},
   url = {https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/rcm.10087},
   volume = {39},
   year = {2025}
}

TY  - JOUR
ID  - 2557759
AU  - Nartey, Linda K - Mikhael, Abanoub - Yang, Hyojik - Šmajs, David - Ernst, Robert K - Petrosova, Helena - Goodlett, David R
PY  - 2025
TI  - Structural Characterization of Pragia fontium Lipid A by MALDI Mass Spectrometry
JF  - Rapid Communications in Mass Spectrometry
VL  - 39
IS  - 18
SP  - 1-4
EP  - 1-4
PB  - Wiley
SN  - 09514198
KW  - Lipid A
KW  - MALDI-TOF MS
KW  - Ozonation
KW  - P. fontium
UR  - https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/rcm.10087
N2  - Here, we report the first structural characterization of lipid A from Pragia fontium isolated from well water in South Bohemia, Czech Republic, in 1982, using our Fast Lipid Analysis Technique (FLAT) and its tandem mass spectrometry version, FLATn. P. fontium is a non-pathogenic environmental bacterium within the Enterobacteriaceae family, which is in the same genera as clinically important species Escherichia, Klebsiella, and Salmonella. P. fontium is not known to cause disease; however, studying its lipid A expands our current understanding of the structural diversity of lipid A within the Enterobacteriaceae beyond clinically relevant species and particularly in underexplored environmental isolates. Understanding such variation provides insight into evolutionary adaptations and structural features critical for environmental survival and immune recognition. Our analysis identified the major P. fontium lipid A molecule, distinguishing it from other Enterobacteriaceae. Lipid A is the bioactive component of lipopolysaccharide (LPS), essential for the structural integrity of Gram-negative bacterial outer membranes and a key modulator of host immune responses. Although the lipid A structures of many pathogenic bacteria have been extensively studied, limited information is available on non-pathogenic or environmental isolates, such as P. fontium. These findings provide new insights into the structural biology of this organism and may have implications for its interaction with the environment and host immune responses.
ER  -

NARTEY, Linda K; Abanoub MIKHAEL; Hyojik YANG; David ŠMAJS; Robert K ERNST; Helena PETROSOVA a David R GOODLETT. Structural Characterization of Pragia fontium Lipid A by MALDI Mass Spectrometry. \textit{Rapid Communications in Mass Spectrometry}. HOBOKEN: Wiley, 2025, roč.~39, č.~18, s.~1-4. ISSN~0951-4198. Dostupné z: https://doi.org/10.1002/rcm.10087.

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