Gyrodactylus PARASITES INDUCING DIFFERENTIAL EXPRESSION OF
IMMUNITY-ASSOCIATED GENES IN SEXUAL AND GYNOGENETIC FORMS OF
FRESHWATER INVASIVE GIBEL CARP (Carassius gibelio)

k 2025

Gyrodactylus PARASITES INDUCING DIFFERENTIAL EXPRESSION OF IMMUNITY-ASSOCIATED GENES IN SEXUAL AND GYNOGENETIC FORMS OF FRESHWATER INVASIVE GIBEL CARP (Carassius gibelio)

VETEŠNÍKOVÁ ŠIMKOVÁ, Andrea; Florian JAQUES; Markéta ONDRAČKOVÁ; Lukáš VETEŠNÍK; Tomáš TICHOPÁD et al.

Základní údaje

Originální název

Gyrodactylus PARASITES INDUCING DIFFERENTIAL EXPRESSION OF IMMUNITY-ASSOCIATED GENES IN SEXUAL AND GYNOGENETIC FORMS OF FRESHWATER INVASIVE GIBEL CARP (Carassius gibelio)

Autoři

VETEŠNÍKOVÁ ŠIMKOVÁ, Andrea; Florian JAQUES; Markéta ONDRAČKOVÁ; Lukáš VETEŠNÍK a Tomáš TICHOPÁD

Vydání

XI International Symposium on Fish Parasites, Merida, Mexiko, 2025

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

10613 Zoology

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Gibel carp; asexual reproduction; Monogenea; Gyrodactylus; RNAseq; immunity

Štítky

14314020

Změněno: 8. 4. 2026 14:48, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Parasite-mediated selection is considered one of the potential mechanisms contributing to the coexistence of asexual-sexual complexes in nature. Gibel carp (Carassius gibelio), is an invasive cyprinid fish species in Europe. Asexual form (gynogenetic triploid females) and sexual form often coexist in the same populations, multiple mechanisms have been hypothesized to explain this coexistence. The gynogenetic and sexual specimens originated from artificial breeding were used for experimental infection by monogeneans. Among monogenean parasites infecting gibel carp, Gyrodactylus longoacuminatus was the dominant species identified on the skin, fin and gills. The transcriptome profile of the spleen was analyzed to reveal the differentially expressed immunity-associated genes related to monogenean infection. The high G. longoacuminatus was reported in both gynogenetic and sexual forms. The higher variability in parasite load was reported in sexual specimens when compared to gynogenetic females. Sexual specimens were more infected by gyrodactylids on the body surface, whilst no difference in Gyrodactylus load on fins between sexual and gynogenetic gibel carp was found. More than 30 GO terms related to immunity were identified. Similar GO terms related to immunity were significantly enriched in infected fish compared to non-infected ones in both sexual and gynogenetic gibel carp. We identified more than 90 immune genes using interaction model (interaction between fish group and treatment), and we inferred the differences in the differential gene expression between sexual and gynogenetic specimens, suggesting that molecular mechanisms associated with immunity potentially contribute to the coexistence of sexual and gynogenetic gibel carp.

Návaznosti

GA22-27023S, projekt VaV

Název: Evoluční a ekologické mechanizmy koexistence asexuální a sexuální reprodukce u ryb

Investor: Grantová agentura ČR, Evolutionary and ecological mechanisms of the coexistence of asexual and sexual reproduction in fish

Citovat

VETEŠNÍKOVÁ ŠIMKOVÁ, Andrea; Florian JAQUES; Markéta ONDRAČKOVÁ; Lukáš VETEŠNÍK a Tomáš TICHOPÁD. Gyrodactylus PARASITES INDUCING DIFFERENTIAL EXPRESSION OF IMMUNITY-ASSOCIATED GENES IN SEXUAL AND GYNOGENETIC FORMS OF FRESHWATER INVASIVE GIBEL CARP (Carassius gibelio). In XI International Symposium on Fish Parasites, Merida, Mexiko. 2025.

@proceedings{2557766,
   author = {Vetešníková Šimková, Andrea and Jaques, Florian and Ondračková, Markéta and Vetešník, Lukáš and Tichopád, Tomáš},
   booktitle = {XI International Symposium on Fish Parasites, Merida, Mexiko},
   keywords = {Gibel carp; asexual reproduction; Monogenea; Gyrodactylus; RNAseq; immunity},
   language = {eng},
   title = {Gyrodactylus PARASITES INDUCING DIFFERENTIAL EXPRESSION OF IMMUNITY-ASSOCIATED GENES IN SEXUAL AND GYNOGENETIC FORMS OF FRESHWATER INVASIVE GIBEL CARP (Carassius gibelio)},
   year = {2025}
}

TY  - CONF
ID  - 2557766
AU  - Vetešníková Šimková, Andrea - Jaques, Florian - Ondračková, Markéta - Vetešník, Lukáš - Tichopád, Tomáš
PY  - 2025
TI  - Gyrodactylus PARASITES INDUCING DIFFERENTIAL EXPRESSION OF IMMUNITY-ASSOCIATED GENES IN SEXUAL AND GYNOGENETIC FORMS OF FRESHWATER INVASIVE GIBEL CARP (Carassius gibelio)
KW  - Gibel carp
KW  - asexual reproduction
KW  - Monogenea
KW  - Gyrodactylus
KW  - RNAseq
KW  - immunity
N2  - Parasite-mediated selection is considered one of the potential mechanisms contributing to the coexistence of asexual-sexual complexes in nature. Gibel carp (Carassius gibelio), is an invasive cyprinid fish species in Europe. Asexual form (gynogenetic triploid females) and sexual form often coexist in the same populations, multiple mechanisms have been hypothesized to explain this coexistence. The gynogenetic and sexual specimens originated from artificial breeding were used for experimental infection by monogeneans. Among monogenean parasites infecting gibel carp, Gyrodactylus longoacuminatus was the dominant species identified on the skin, fin and gills. The transcriptome profile of the spleen was analyzed to reveal the differentially expressed immunity-associated genes related to monogenean infection. The high G. longoacuminatus was reported in both gynogenetic and sexual forms. The higher variability in parasite load was reported in sexual specimens when compared to gynogenetic females. Sexual specimens were more infected by gyrodactylids on the body surface, whilst no difference in Gyrodactylus load on fins between sexual and gynogenetic gibel carp was found. More than 30 GO terms related to immunity were identified. Similar GO terms related to immunity were significantly enriched in infected fish compared to non-infected ones in both sexual and gynogenetic gibel carp. We identified more than 90 immune genes using interaction model (interaction between fish group and treatment), and we inferred the differences in the differential gene expression between sexual and gynogenetic specimens, suggesting that molecular mechanisms associated with immunity potentially contribute to the coexistence of sexual and gynogenetic gibel carp.
ER  -

VETEŠNÍKOVÁ ŠIMKOVÁ, Andrea; Florian JAQUES; Markéta ONDRAČKOVÁ; Lukáš VETEŠNÍK a Tomáš TICHOPÁD. Gyrodactylus PARASITES INDUCING DIFFERENTIAL EXPRESSION OF IMMUNITY-ASSOCIATED GENES IN SEXUAL AND GYNOGENETIC FORMS OF FRESHWATER INVASIVE GIBEL CARP (Carassius gibelio). In \textit{XI International Symposium on Fish Parasites, Merida, Mexiko}. 2025.

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