Role of the sodium/calcium exchanger type 3 in cancer cells

J 2025

Role of the sodium/calcium exchanger type 3 in cancer cells

GALVANKOVA, Kristina; Ingeborg REZUCHOVA; Ladislav KLENA; Marian GRMAN; Simova GAZOVA et al.

Základní údaje

Originální název

Role of the sodium/calcium exchanger type 3 in cancer cells

Autoři

GALVANKOVA, Kristina; Ingeborg REZUCHOVA; Ladislav KLENA; Marian GRMAN; Simova GAZOVA; Veronika LISKOVA; Zuzana KOZOVSKA; Ladislav ROLLER; Petr BABULA a Oľga KRIŽANOVÁ

Vydání

European Journal of Cell Biology, MUNICH, ELSEVIER GMBH, 2025, 0171-9335

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.300 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/25:00143828

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Sodium/calcium exchanger type 3; Proliferation; Migration; Breast cancer cell lines; Colorectal cancer cell line; Cervical cancer cell line; Mitochondrial respiration

Štítky

14110515, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 3. 2026 13:07, Mgr. Tereza Miškechová

Anotace

V originále

The sodium/calcium exchanger (NCX) type 1 has been well described in various cancers, but little is known about the other two NCX types (NCX2 and NCX3). In this study, we used the selective blocker of NCX3 - YM244769 to investigate changes in apoptosis induction, migration, proliferation, intracellular calcium and ATP in four cancer cell lines - DLD1, HeLa, MDA-MB-231 and JIMT1. In all four cell lines we observed a concentration-dependent increase in the number of apoptotic cells, as well as reduced migration and proliferation. Induction of hypoxic conditions did not alter the response of these cells to YM-244769 in any of the abovementioned parameters. These results indicate the role of NCX3 in cancer cell migration, proliferation and apoptosis, as inhibition of NCX1 by the specific blocker SEA0400 had no significant effect on these parameters. However, we verified the effect of NCX3 inhibition by using CRISPR/Cas9 to generate clones in which the SLC8A3 (NCX3) gene was deleted, and we obtained the same results. In addition, mitochondrial respiration was impaired in the clones with NCX3 knocked-out, suggesting that NCX3 also play a role in bioenergetics. In conclusion, we have clearly shown that NCX3 plays an important anti-apoptotic, pro-migratory and proliferative role in the cancer cells by affecting mitochondrial bioenergetics, thus supporting their survival and fate.

Citovat

GALVANKOVA, Kristina; Ingeborg REZUCHOVA; Ladislav KLENA; Marian GRMAN; Simova GAZOVA; Veronika LISKOVA; Zuzana KOZOVSKA; Ladislav ROLLER; Petr BABULA a Oľga KRIŽANOVÁ. Role of the sodium/calcium exchanger type 3 in cancer cells. European Journal of Cell Biology. MUNICH: ELSEVIER GMBH, 2025, roč. 104, č. 2, s. 1-11. ISSN 0171-9335. Dostupné z: https://doi.org/10.1016/j.ejcb.2025.151493.

@article{2557821,
   author = {Galvankova, Kristina and Rezuchova, Ingeborg and Klena, Ladislav and Grman, Marian and Gazova, Simova and Liskova, Veronika and Kozovska, Zuzana and Roller, Ladislav and Babula, Petr and Križanová, Oľga},
   article_location = {MUNICH},
   article_number = {2},
   doi = {https://doi.org/10.1016/j.ejcb.2025.151493},
   keywords = {Sodium/calcium exchanger type 3; Proliferation; Migration; Breast cancer cell lines; Colorectal cancer cell line; Cervical cancer cell line; Mitochondrial respiration},
   language = {eng},
   issn = {0171-9335},
   journal = {European Journal of Cell Biology},
   title = {Role of the sodium/calcium exchanger type 3 in cancer cells},
   url = {https://www.sciencedirect.com/science/article/pii/S0171933525000184?via%3Dihub},
   volume = {104},
   year = {2025}
}

TY  - JOUR
ID  - 2557821
AU  - Galvankova, Kristina - Rezuchova, Ingeborg - Klena, Ladislav - Grman, Marian - Gazova, Simova - Liskova, Veronika - Kozovska, Zuzana - Roller, Ladislav - Babula, Petr - Križanová, Oľga
PY  - 2025
TI  - Role of the sodium/calcium exchanger type 3 in cancer cells
JF  - European Journal of Cell Biology
VL  - 104
IS  - 2
SP  - 1-11
EP  - 1-11
PB  - ELSEVIER GMBH
SN  - 01719335
KW  - Sodium/calcium exchanger type 3
KW  - Proliferation
KW  - Migration
KW  - Breast cancer cell lines
KW  - Colorectal cancer cell line
KW  - Cervical cancer cell line
KW  - Mitochondrial respiration
UR  - https://www.sciencedirect.com/science/article/pii/S0171933525000184?via%3Dihub
N2  - The sodium/calcium exchanger (NCX) type 1 has been well described in various cancers, but little is known about the other two NCX types (NCX2 and NCX3). In this study, we used the selective blocker of NCX3 - YM244769 to investigate changes in apoptosis induction, migration, proliferation, intracellular calcium and ATP in four cancer cell lines - DLD1, HeLa, MDA-MB-231 and JIMT1. In all four cell lines we observed a concentration-dependent increase in the number of apoptotic cells, as well as reduced migration and proliferation. Induction of hypoxic conditions did not alter the response of these cells to YM-244769 in any of the abovementioned parameters. These results indicate the role of NCX3 in cancer cell migration, proliferation and apoptosis, as inhibition of NCX1 by the specific blocker SEA0400 had no significant effect on these parameters. However, we verified the effect of NCX3 inhibition by using CRISPR/Cas9 to generate clones in which the SLC8A3 (NCX3) gene was deleted, and we obtained the same results. In addition, mitochondrial respiration was impaired in the clones with NCX3 knocked-out, suggesting that NCX3 also play a role in bioenergetics. In conclusion, we have clearly shown that NCX3 plays an important anti-apoptotic, pro-migratory and proliferative role in the cancer cells by affecting mitochondrial bioenergetics, thus supporting their survival and fate.
ER  -

GALVANKOVA, Kristina; Ingeborg REZUCHOVA; Ladislav KLENA; Marian GRMAN; Simova GAZOVA; Veronika LISKOVA; Zuzana KOZOVSKA; Ladislav ROLLER; Petr BABULA a Oľga KRIŽANOVÁ. Role of the sodium/calcium exchanger type 3 in cancer cells. \textit{European Journal of Cell Biology}. MUNICH: ELSEVIER GMBH, 2025, roč.~104, č.~2, s.~1-11. ISSN~0171-9335. Dostupné z: https://doi.org/10.1016/j.ejcb.2025.151493.

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