Identification of polycyclic aromatic hydrocarbons (PAHs)
collected through passive air sampling and their effects in
vitro on human pulmonary epithelial cell lines

J 2025

Identification of polycyclic aromatic hydrocarbons (PAHs) collected through passive air sampling and their effects in vitro on human pulmonary epithelial cell lines

LETELIER, Pablo; Karla Andrea POZO; Nicolas SAAVEDRA; Marcelo GARCES; Luis A. SALAZAR et al.

Základní údaje

Originální název

Identification of polycyclic aromatic hydrocarbons (PAHs) collected through passive air sampling and their effects in vitro on human pulmonary epithelial cell lines

Autoři

LETELIER, Pablo; Karla Andrea POZO; Nicolas SAAVEDRA; Marcelo GARCES; Luis A. SALAZAR; Gabriel ROJAS; Viviana BURGOS; Marco CAMPOS a Neftali GUZMAN

Vydání

JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING, PHILADELPHIA, TAYLOR & FRANCIS INC, 2025, 1093-4529

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.100 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/25:00143927

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Environmental pollution; polycyclic aromatic hydrocarbons; human bronchial epithelial cells; passive air samplers with polyurethane foam; cytotoxicity

Štítky

143180, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 3. 2026 11:29, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The presence of polycyclic aromatic hydrocarbons (PAHs) in ambient air is mainly linked to anthropogenic activities, particularly fossil fuel use and residential wood combustion, posing risks to human health. This study evaluated atmospheric PAHs in Temuco, Chile, characterized their spatial and seasonal distribution, and investigated the in vitro effects of environmentally relevant PAH mixtures on human bronchial epithelial cells (BEAS-2B). Passive air samplers equipped with polyurethane foam (PUF) disks were deployed at two urban sites (Universidad Católica de Temuco and Padre Las Casas) and one rural site (Maquehue sector). Sampling covered summer and fall–early winter, with seasonal assessment conducted in Padre Las Casas. PAHs were quantified by gas chromatography–mass spectrometry (GC–MS), and cell viability was assessed using the MTS assay after exposure to defined PAH mixtures. Spatial variability was observed, with phenanthrene, fluoranthene, and pyrene predominating in urban areas and increasing during winter, while dibenzo(a,h)anthracene was detected exclusively in the rural sector. In BEAS-2B cells, PAH exposure caused a dose and time-dependent reduction in viability, reaching significance at 20 and 28 µM after 48 and 72 h. These findings highlight PAH persistence and potential adverse effects on respiratory epithelial cells, underscoring the need to reduce population exposure.

Citovat

LETELIER, Pablo; Karla Andrea POZO; Nicolas SAAVEDRA; Marcelo GARCES; Luis A. SALAZAR; Gabriel ROJAS; Viviana BURGOS; Marco CAMPOS a Neftali GUZMAN. Identification of polycyclic aromatic hydrocarbons (PAHs) collected through passive air sampling and their effects in vitro on human pulmonary epithelial cell lines. JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING. PHILADELPHIA: TAYLOR & FRANCIS INC, 2025, roč. 60, č. 12, s. 633-643. ISSN 1093-4529. Dostupné z: https://doi.org/10.1080/10934529.2026.2613198.

@article{2559452,
   author = {Letelier, Pablo and Pozo, Karla Andrea and Saavedra, Nicolas and Garces, Marcelo and Salazar, Luis A. and Rojas, Gabriel and Burgos, Viviana and Campos, Marco and Guzman, Neftali},
   article_location = {PHILADELPHIA},
   article_number = {12},
   doi = {https://doi.org/10.1080/10934529.2026.2613198},
   keywords = {Environmental pollution; polycyclic aromatic hydrocarbons; human bronchial epithelial cells; passive air samplers with polyurethane foam; cytotoxicity},
   language = {eng},
   issn = {1093-4529},
   journal = {JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING},
   title = {Identification of polycyclic aromatic hydrocarbons (PAHs) collected through passive air sampling and their effects in vitro on human pulmonary epithelial cell lines},
   url = {https://doi.org/10.1080/10934529.2026.2613198},
   volume = {60},
   year = {2025}
}

TY  - JOUR
ID  - 2559452
AU  - Letelier, Pablo - Pozo, Karla Andrea - Saavedra, Nicolas - Garces, Marcelo - Salazar, Luis A. - Rojas, Gabriel - Burgos, Viviana - Campos, Marco - Guzman, Neftali
PY  - 2025
TI  - Identification of polycyclic aromatic hydrocarbons (PAHs) collected through passive air sampling and their effects in vitro on human pulmonary epithelial cell lines
JF  - JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING
VL  - 60
IS  - 12
SP  - 633-643
EP  - 633-643
PB  - TAYLOR & FRANCIS INC
SN  - 10934529
KW  - Environmental pollution
KW  - polycyclic aromatic hydrocarbons
KW  - human bronchial epithelial cells
KW  - passive air samplers with polyurethane foam
KW  - cytotoxicity
UR  - https://doi.org/10.1080/10934529.2026.2613198
N2  - The presence of polycyclic aromatic hydrocarbons (PAHs) in ambient air is mainly linked to anthropogenic activities, particularly fossil fuel use and residential wood combustion, posing risks to human health. This study evaluated atmospheric PAHs in Temuco, Chile, characterized their spatial and seasonal distribution, and investigated the in vitro effects of environmentally relevant PAH mixtures on human bronchial epithelial cells (BEAS-2B). Passive air samplers equipped with polyurethane foam (PUF) disks were deployed at two urban sites (Universidad Católica de Temuco and Padre Las Casas) and one rural site (Maquehue sector). Sampling covered summer and fall–early winter, with seasonal assessment conducted in Padre Las Casas. PAHs were quantified by gas chromatography–mass spectrometry (GC–MS), and cell viability was assessed using the MTS assay after exposure to defined PAH mixtures. Spatial variability was observed, with phenanthrene, fluoranthene, and pyrene predominating in urban areas and increasing during winter, while dibenzo(a,h)anthracene was detected exclusively in the rural sector. In BEAS-2B cells, PAH exposure caused a dose and time-dependent reduction in viability, reaching significance at 20 and 28 µM after 48 and 72 h. These findings highlight PAH persistence and potential adverse effects on respiratory epithelial cells, underscoring the need to reduce population exposure.
ER  -

LETELIER, Pablo; Karla Andrea POZO; Nicolas SAAVEDRA; Marcelo GARCES; Luis A. SALAZAR; Gabriel ROJAS; Viviana BURGOS; Marco CAMPOS a Neftali GUZMAN. Identification of polycyclic aromatic hydrocarbons (PAHs) collected through passive air sampling and their effects in vitro on human pulmonary epithelial cell lines. \textit{JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES \&{} ENVIRONMENTAL ENGINEERING}. PHILADELPHIA: TAYLOR \&{} FRANCIS INC, 2025, roč.~60, č.~12, s.~633-643. ISSN~1093-4529. Dostupné z: https://doi.org/10.1080/10934529.2026.2613198.

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