Natural Phenolics Disrupt Microbial Communication by Inhibiting
Quorum Sensing

J 2025

Natural Phenolics Disrupt Microbial Communication by Inhibiting Quorum Sensing

HELCMAN, Martin; Karel ŠMEJKAL; Marie ČULENOVÁ; Tibor BERES; Jakub TREML et al.

Základní údaje

Originální název

Natural Phenolics Disrupt Microbial Communication by Inhibiting Quorum Sensing

Autoři

HELCMAN, Martin; Karel ŠMEJKAL; Marie ČULENOVÁ; Tibor BERES a Jakub TREML

Vydání

Microorganisms, Basel, MDPI, 2025, 2076-2607

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.200 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Farmaceutická fakulta

Klíčová slova anglicky

antibacterial; Cannabis sativa; Morus alba; phenolic; prenyl; quorum sensing

Štítky

rivok, ÚMF, ÚPL

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 3. 2026 16:56, Mgr. Irena Doubková

Anotace

V originále

Quorum sensing, a bacterial cell-to-cell communication mechanism, plays a key role in bacterial virulence and biofilm formation. Targeting quorum-sensing pathways represents a promising strategy for the development of novel antibacterial agents. This study evaluated the anti-quorum-sensing activities of 18 natural compounds, including cannabinoids, arylbenzofurans, flavonoids, caffeine, and chlorogenic acid, using the luminescent biosensor strain Vibrio harveyi MM30. V. harveyi MM30, a mutant strain deficient in the production of autoinducer-2 (AI-2) but responsive to exogenous AI-2, was used to assess the activity of test compounds on the AI-2 receptor pathway. Test compounds were incubated in AI-2-containing media, and luminescence was measured to evaluate quorum-sensing inhibition. Comparisons were made in the absence of AI-2 to determine AI-2-independent inhibitory activity. The most active compounds were further tested on methicillin-resistant Staphylococcus aureus (MRSA 7112) to determine their effects on AI-2 production in spent media. Among the tested compounds, the non-prenylated arylbenzofuran moracin M and the prenylated arylbenzofuran moracin C exhibited significant quorum-sensing inhibitory activity in the AI-2-mediated pathway. None of the test compounds significantly inhibited quorum sensing in the absence of AI-2. Five compounds (cannabigerol, cannabidiol, cannabigerolic acid, moracin M, and moracin C) were selected for further investigation in MRSA 7112 cultures. The spent media from MRSA 7112 cultures treated with moracin M (16, 32, 64 mu g/mL) and cannabigerolic acid (16 mu g/mL) showed significant inhibition of AI-2 production when transferred to V. harveyi MM30 cultures. Moracin M and cannabigerolic acid demonstrated potential as quorum-sensing inhibitors by targeting AI-2 production and signalling pathways in MRSA 7112 and V. harveyi. These findings suggest their potential for further development as antibacterial agents targeting quorum-sensing mechanisms.

Citovat

HELCMAN, Martin; Karel ŠMEJKAL; Marie ČULENOVÁ; Tibor BERES a Jakub TREML. Natural Phenolics Disrupt Microbial Communication by Inhibiting Quorum Sensing. Microorganisms. Basel: MDPI, 2025, roč. 13, č. 2, s. 1-15. ISSN 2076-2607. Dostupné z: https://doi.org/10.3390/microorganisms13020287.

@article{2559922,
   author = {Helcman, Martin and Šmejkal, Karel and Čulenová, Marie and Beres, Tibor and Treml, Jakub},
   article_location = {Basel},
   article_number = {2},
   doi = {https://doi.org/10.3390/microorganisms13020287},
   keywords = {antibacterial; Cannabis sativa; Morus alba; phenolic; prenyl; quorum sensing},
   language = {eng},
   issn = {2076-2607},
   journal = {Microorganisms},
   title = {Natural Phenolics Disrupt Microbial Communication by Inhibiting Quorum Sensing},
   url = {https://www.mdpi.com/2076-2607/13/2/287},
   volume = {13},
   year = {2025}
}

TY  - JOUR
ID  - 2559922
AU  - Helcman, Martin - Šmejkal, Karel - Čulenová, Marie - Beres, Tibor - Treml, Jakub
PY  - 2025
TI  - Natural Phenolics Disrupt Microbial Communication by Inhibiting Quorum Sensing
JF  - Microorganisms
VL  - 13
IS  - 2
SP  - 1-15
EP  - 1-15
PB  - MDPI
SN  - 20762607
KW  - antibacterial
KW  - Cannabis sativa
KW  - Morus alba
KW  - phenolic
KW  - prenyl
KW  - quorum sensing
UR  - https://www.mdpi.com/2076-2607/13/2/287
N2  - Quorum sensing, a bacterial cell-to-cell communication mechanism, plays a key role in bacterial virulence and biofilm formation. Targeting quorum-sensing pathways represents a promising strategy for the development of novel antibacterial agents. This study evaluated the anti-quorum-sensing activities of 18 natural compounds, including cannabinoids, arylbenzofurans, flavonoids, caffeine, and chlorogenic acid, using the luminescent biosensor strain Vibrio harveyi MM30. V. harveyi MM30, a mutant strain deficient in the production of autoinducer-2 (AI-2) but responsive to exogenous AI-2, was used to assess the activity of test compounds on the AI-2 receptor pathway. Test compounds were incubated in AI-2-containing media, and luminescence was measured to evaluate quorum-sensing inhibition. Comparisons were made in the absence of AI-2 to determine AI-2-independent inhibitory activity. The most active compounds were further tested on methicillin-resistant Staphylococcus aureus (MRSA 7112) to determine their effects on AI-2 production in spent media. Among the tested compounds, the non-prenylated arylbenzofuran moracin M and the prenylated arylbenzofuran moracin C exhibited significant quorum-sensing inhibitory activity in the AI-2-mediated pathway. None of the test compounds significantly inhibited quorum sensing in the absence of AI-2. Five compounds (cannabigerol, cannabidiol, cannabigerolic acid, moracin M, and moracin C) were selected for further investigation in MRSA 7112 cultures. The spent media from MRSA 7112 cultures treated with moracin M (16, 32, 64 mu g/mL) and cannabigerolic acid (16 mu g/mL) showed significant inhibition of AI-2 production when transferred to V. harveyi MM30 cultures. Moracin M and cannabigerolic acid demonstrated potential as quorum-sensing inhibitors by targeting AI-2 production and signalling pathways in MRSA 7112 and V. harveyi. These findings suggest their potential for further development as antibacterial agents targeting quorum-sensing mechanisms.
ER  -

HELCMAN, Martin; Karel ŠMEJKAL; Marie ČULENOVÁ; Tibor BERES a Jakub TREML. Natural Phenolics Disrupt Microbial Communication by Inhibiting Quorum Sensing. \textit{Microorganisms}. Basel: MDPI, 2025, roč.~13, č.~2, s.~1-15. ISSN~2076-2607. Dostupné z: https://doi.org/10.3390/microorganisms13020287.

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