The Role of Sonication in Improving the Detection of
Periprosthetic Joint Infections: a Prospective Analysis

J 2025

The Role of Sonication in Improving the Detection of Periprosthetic Joint Infections: a Prospective Analysis

RAPI, Jakub; Vasileios APOSTOLOPOULOS; Filip RŮŽIČKA; Martina VANĚRKOVÁ; Pavel BRANČÍK et al.

Základní údaje

Originální název

The Role of Sonication in Improving the Detection of Periprosthetic Joint Infections: a Prospective Analysis

Autoři

RAPI, Jakub ; Vasileios APOSTOLOPOULOS; Filip RŮŽIČKA ; Martina VANĚRKOVÁ; Pavel BRANČÍK a Tomáš TOMÁŠ

Vydání

Acta chirurgiae orthopaedicae et traumatologiae čechoslovaca, Praha, Česká lékařská společnost J.E. Purkyně, 2025, 0001-5415

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30211 Orthopaedics

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 0.400 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

periprosthetic joint infection; polymerase chain reaction; sonification; sonication-assisted PCR; arthroplasty

Štítky

14110113, 14110123, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 3. 2026 10:09, Mgr. Tereza Miškechová

Anotace

V originále

Purpose of the study: The diagnosis of periprosthetic joint infection (PJI) can be particularly challenging in cases of low-grade chronic infection. The suspicion of infection is typically confirmed through cultures of synovial fluid and periprosthetic tissue. However, these methods may not always detect low-grade infections, which can lead to persistent infection and early failure of the prosthesis. The purpose of this study was to evaluate the effectiveness of sonication in enhancing the detection of PJI using polymerase chain reaction (PCR) analysis. Material and methods: A prospective cohort of 26 patients, suspected of having mitigated PJI, underwent surgery at the First Department of Orthopaedic Surgery, St. Anne's University Hospital in Brno between 2019 and 2024. The cohort included 16 women and 10 men, aged 56 to 82 years, with infections involving hip (11 cases) or knee prostheses (15 cases). Standard PCR and sonication followed by PCR were used to confirm PJI. Results: In 20 out of 26 cases, both standard PCR and sonication-assisted PCR detected the infection(p= 0.014). However, in 6 cases, standard PCR failed to identify the pathogen, whereas sonication followed by PCR confirmed the infection. Among these, 4 cases had significantly positive results, and 2 showed weak positivity. The most common pathogens detected were coagulase-negative Staphylococcus (12 cases), followed by Staphylococcus aureus, Pseudomonas aeruginosa, and others. Conclusions: The findings of this study indicate that the integration of sonication with PCR markedly enhances the detection of PJI, especially in instances where standard PCR techniques may be insufficient, such as in low-grade chronic infections.

Citovat

RAPI, Jakub; Vasileios APOSTOLOPOULOS; Filip RŮŽIČKA; Martina VANĚRKOVÁ; Pavel BRANČÍK a Tomáš TOMÁŠ. The Role of Sonication in Improving the Detection of Periprosthetic Joint Infections: a Prospective Analysis. Acta chirurgiae orthopaedicae et traumatologiae čechoslovaca. Praha: Česká lékařská společnost J.E. Purkyně, 2025, roč. 92, č. 4, s. 187-194. ISSN 0001-5415. Dostupné z: https://doi.org/10.55095/achot2024/061.

@article{2559939,
   author = {Rapi, Jakub and Apostolopoulos, Vasileios and Růžička, Filip and Vaněrková, Martina and Brančík, Pavel and Tomáš, Tomáš},
   article_location = {Praha},
   article_number = {4},
   doi = {https://doi.org/10.55095/achot2024/061},
   keywords = {periprosthetic joint infection; polymerase chain reaction; sonification; sonication-assisted PCR; arthroplasty},
   language = {eng},
   issn = {0001-5415},
   journal = {Acta chirurgiae orthopaedicae et traumatologiae čechoslovaca},
   title = {The Role of Sonication in Improving the Detection of Periprosthetic Joint Infections: a Prospective Analysis},
   url = {https://achot.cz/artkey/ach-202504-0001_the-role-of-sonication-in-improving-the-detection-of-periprosthetic-joint-infections-a-prospective-analysis.php},
   volume = {92},
   year = {2025}
}

TY  - JOUR
ID  - 2559939
AU  - Rapi, Jakub - Apostolopoulos, Vasileios - Růžička, Filip - Vaněrková, Martina - Brančík, Pavel - Tomáš, Tomáš
PY  - 2025
TI  - The Role of Sonication in Improving the Detection of Periprosthetic Joint Infections: a Prospective Analysis
JF  - Acta chirurgiae orthopaedicae et traumatologiae čechoslovaca
VL  - 92
IS  - 4
SP  - 187-194
EP  - 187-194
PB  - Česká lékařská společnost J.E. Purkyně
SN  - 00015415
KW  - periprosthetic joint infection
KW  - polymerase chain reaction
KW  - sonification
KW  - sonication-assisted PCR
KW  - arthroplasty
UR  - https://achot.cz/artkey/ach-202504-0001_the-role-of-sonication-in-improving-the-detection-of-periprosthetic-joint-infections-a-prospective-analysis.php
N2  - Purpose of the study: The diagnosis of periprosthetic joint infection (PJI) can be particularly challenging in cases of low-grade chronic infection. The suspicion of infection is typically confirmed through cultures of synovial fluid and periprosthetic tissue. However, these methods may not always detect low-grade infections, which can lead to persistent infection and early failure of the prosthesis. The purpose of this study was to evaluate the effectiveness of sonication in enhancing the detection of PJI using polymerase chain reaction (PCR) analysis. Material and methods: A prospective cohort of 26 patients, suspected of having mitigated PJI, underwent surgery at the First Department of Orthopaedic Surgery, St. Anne's University Hospital in Brno between 2019 and 2024. The cohort included 16 women and 10 men, aged 56 to 82 years, with infections involving hip (11 cases) or knee prostheses (15 cases). Standard PCR and sonication followed by PCR were used to confirm PJI. Results: In 20 out of 26 cases, both standard PCR and sonication-assisted PCR detected the infection(p= 0.014). However, in 6 cases, standard PCR failed to identify the pathogen, whereas sonication followed by PCR confirmed the infection. Among these, 4 cases had significantly positive results, and 2 showed weak positivity. The most common pathogens detected were coagulase-negative Staphylococcus (12 cases), followed by Staphylococcus aureus, Pseudomonas aeruginosa, and others. Conclusions: The findings of this study indicate that the integration of sonication with PCR markedly enhances the detection of PJI, especially in instances where standard PCR techniques may be insufficient, such as in low-grade chronic infections.
ER  -

RAPI, Jakub; Vasileios APOSTOLOPOULOS; Filip RŮŽIČKA; Martina VANĚRKOVÁ; Pavel BRANČÍK a Tomáš TOMÁŠ. The Role of Sonication in Improving the Detection of Periprosthetic Joint Infections: a Prospective Analysis. \textit{Acta chirurgiae orthopaedicae et traumatologiae čechoslovaca}. Praha: Česká lékařská společnost J.E. Purkyně, 2025, roč.~92, č.~4, s.~187-194. ISSN~0001-5415. Dostupné z: https://doi.org/10.55095/achot2024/061.

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