2024
Unique structural attributes of the PSI-NDH supercomplex in <i>Physcomitrium patens</i>
OPATIKOVA, Monika a Roman KOURILZákladní údaje
Originální název
Unique structural attributes of the PSI-NDH supercomplex in <i>Physcomitrium patens</i>
Autoři
OPATIKOVA, Monika a Roman KOURIL
Vydání
Plant Journal, Hoboken (USA), Wiley-Blackwell, 2024, 0960-7412
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10611 Plant sciences, botany
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 5.700
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:90242/24:00143949
Organizační jednotka
CIISB III
UT WoS
EID Scopus
Klíčová slova anglicky
PSI-NDH supercomplex; transmission electron microscopy; single particle analysis; <italic>Physcomitrium patens</italic>; LHCA5; cyclic electron transport
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 12. 3. 2026 12:25, Mgr. Eva Dubská
Anotace
V originále
Cyclic electron transport around photosystem I (PSI) is essential for the protection of the photosynthetic apparatus in plants under diverse light conditions. This process is primarily mediated by Proton Gradient Regulation 5 protein/Proton Gradient Regulation 5-like photosynthetic phenotype 1 protein (PGR5/PGRL1) and NADH dehydrogenase-like complex (NDH). In angiosperms, NDH interacts with two PSI complexes through distinct monomeric antennae, LHCA5 and LHCA6, which is crucial for its higher stability under variable light conditions. This interaction represents an advanced evolutionary stage and offers limited insight into the origin of the PSI-NDH supercomplex in evolutionarily older organisms. In contrast, the moss Physcomitrium patens (Pp), which retains the lhca5 gene but lacks the lhca6, offers a glimpse into an earlier evolutionary stage of the PSI-NDH supercomplex. Here we present structural evidence of the Pp PSI-NDH supercomplex formation by single particle electron microscopy, demonstrating the unique ability of Pp to bind a single PSI in two different configurations. One configuration closely resembles the angiosperm model, whereas the other exhibits a novel PSI orientation, rotated clockwise. This structural flexibility in Pp is presumably enabled by the variable incorporation of LHCA5 within PSI and is indicative of an early evolutionary adaptation that allowed for greater diversity at the PSI-NDH interface. Our findings suggest that this variability was reduced as the structural complexity of the NDH complex increased in vascular plants, primarily angiosperms. This study not only clarifies the evolutionary development of PSI-NDH supercomplexes but also highlights the dynamic nature of the adaptive mechanisms of plant photosynthesis.
Návaznosti
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