Insights into the cytotoxicity of Ganoderma adspersum mycelium
extracts against human renal cancer cell lines

J 2026

Insights into the cytotoxicity of Ganoderma adspersum mycelium extracts against human renal cancer cell lines

SCHLOSSEROVA, Nikola; Biagia MUSIO; Maria Noemi SGOBBA; Federica MASTROPIRRO; Antonio RADESCO et al.

Základní údaje

Originální název

Insights into the cytotoxicity of Ganoderma adspersum mycelium extracts against human renal cancer cell lines

Autoři

Vydání

JOURNAL OF AGRICULTURE AND FOOD RESEARCH, AMSTERDAM, ELSEVIER, 2026, 2666-1543

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 6.200 v roce 2024

Označené pro přenos do RIV

Organizační jednotka

Farmaceutická fakulta

DOI

https://doi.org/10.1016/j.jafr.2026.102770

UT WoS

001696538100001

Klíčová slova anglicky

Natural products; Medicinal fungi; Ganoderma; Renal cancer cells; NMR

Štítky

ÚMF

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 3. 2026 16:52, Mgr. Irena Doubková

Anotace

V originále

Ganoderma adspersum is a medicinal fungus known for its bioactive properties, including antibacterial, anticholinesterase, and antioxidant activities. While preliminary research suggests antiproliferative effects on breast cancer cells, its efficacy against other cancer types remains underexplored. This study aims to evaluate the antiproliferative activity of ethanolic extracts of Ganoderma adspersum on human renal cancer cells. Ethanolic extracts were prepared from (i) the liquid culture medium post-fermentation and (ii) the fungal mycelium. Both extracts were tested in vitro on human renal cancer cell lines Caki-1, 786-O and RCC-Shaw, as well as on nontumor human renal HK-2 cells. Notably, we found that only the mycelium-derived ethanolic extract showed significant antiproliferative activity, reducing cell viability by more than 80% in both 786-O and RCC-Shaw cells after 72 h. Concentrations of Gad mycelium extract up to 500 mu g/ml were well tolerated by HK-2 non-tumor renal cells. Nuclear Magnetic Resonance (NMR) spectroscopy revealed marked differences in the composition of the two extracts. Based on the information on the most abundant metabolites in the Gad mycelium extract, specific compounds were tested in vitro to explore their effects on the cell viability. Our findings support the potential of Ganoderma adspersum mycelium extract as a selective, non-toxic source of bioactive compounds with antiproliferative properties within the range of 125 - 500 mu g/ml.

Citovat

SCHLOSSEROVA, Nikola; Biagia MUSIO; Maria Noemi SGOBBA; Federica MASTROPIRRO; Antonio RADESCO; Maria FAVIA; Simona Marianna SANZANI; Stefano TODISCO; Mariateresa VOLPICELLA; De Grassi ANNA; Vito GALLO; Elena CIANI; Ciro Leonardo PIERRI; Jakub TREML a Lorenzo GUERRA. Insights into the cytotoxicity of Ganoderma adspersum mycelium extracts against human renal cancer cell lines. JOURNAL OF AGRICULTURE AND FOOD RESEARCH. AMSTERDAM: ELSEVIER, 2026, roč. 27, 14 s. ISSN 2666-1543. Dostupné z: https://doi.org/10.1016/j.jafr.2026.102770.

@article{2560241,
   author = {Schlosserova, Nikola and Musio, Biagia and Sgobba, Maria Noemi and Mastropirro, Federica and Radesco, Antonio and Favia, Maria and Sanzani, Simona Marianna and Todisco, Stefano and Volpicella, Mariateresa and Anna, De Grassi and Gallo, Vito and Ciani, Elena and Pierri, Ciro Leonardo and Treml, Jakub and Guerra, Lorenzo},
   article_location = {AMSTERDAM},
   doi = {https://doi.org/10.1016/j.jafr.2026.102770},
   keywords = {Natural products; Medicinal fungi; Ganoderma; Renal cancer cells; NMR},
   language = {eng},
   issn = {2666-1543},
   journal = {JOURNAL OF AGRICULTURE AND FOOD RESEARCH},
   title = {Insights into the cytotoxicity of Ganoderma adspersum mycelium extracts against human renal cancer cell lines},
   volume = {27},
   year = {2026}
}

TY  - JOUR
ID  - 2560241
AU  - Schlosserova, Nikola - Musio, Biagia - Sgobba, Maria Noemi - Mastropirro, Federica - Radesco, Antonio - Favia, Maria - Sanzani, Simona Marianna - Todisco, Stefano - Volpicella, Mariateresa - Anna, De Grassi - Gallo, Vito - Ciani, Elena - Pierri, Ciro Leonardo - Treml, Jakub - Guerra, Lorenzo
PY  - 2026
TI  - Insights into the cytotoxicity of Ganoderma adspersum mycelium extracts against human renal cancer cell lines
JF  - JOURNAL OF AGRICULTURE AND FOOD RESEARCH
VL  - 27
PB  - ELSEVIER
SN  - 26661543
KW  - Natural products
KW  - Medicinal fungi
KW  - Ganoderma
KW  - Renal cancer cells
KW  - NMR
N2  - Ganoderma adspersum is a medicinal fungus known for its bioactive properties, including antibacterial, anticholinesterase, and antioxidant activities. While preliminary research suggests antiproliferative effects on breast cancer cells, its efficacy against other cancer types remains underexplored. This study aims to evaluate the antiproliferative activity of ethanolic extracts of Ganoderma adspersum on human renal cancer cells. Ethanolic extracts were prepared from (i) the liquid culture medium post-fermentation and (ii) the fungal mycelium. Both extracts were tested in vitro on human renal cancer cell lines Caki-1, 786-O and RCC-Shaw, as well as on nontumor human renal HK-2 cells. Notably, we found that only the mycelium-derived ethanolic extract showed significant antiproliferative activity, reducing cell viability by more than 80% in both 786-O and RCC-Shaw cells after 72 h. Concentrations of Gad mycelium extract up to 500 mu g/ml were well tolerated by HK-2 non-tumor renal cells. Nuclear Magnetic Resonance (NMR) spectroscopy revealed marked differences in the composition of the two extracts. Based on the information on the most abundant metabolites in the Gad mycelium extract, specific compounds were tested in vitro to explore their effects on the cell viability. Our findings support the potential of Ganoderma adspersum mycelium extract as a selective, non-toxic source of bioactive compounds with antiproliferative properties within the range of 125 - 500 mu g/ml.
ER  -

SCHLOSSEROVA, Nikola; Biagia MUSIO; Maria Noemi SGOBBA; Federica MASTROPIRRO; Antonio RADESCO; Maria FAVIA; Simona Marianna SANZANI; Stefano TODISCO; Mariateresa VOLPICELLA; De Grassi ANNA; Vito GALLO; Elena CIANI; Ciro Leonardo PIERRI; Jakub TREML a Lorenzo GUERRA. Insights into the cytotoxicity of Ganoderma adspersum mycelium extracts against human renal cancer cell lines. \textit{JOURNAL OF AGRICULTURE AND FOOD RESEARCH}. AMSTERDAM: ELSEVIER, 2026, roč.~27, 14 s. ISSN~2666-1543. Dostupné z: https://doi.org/10.1016/j.jafr.2026.102770.

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