Hairy root tissue culture as a tool for studying hormonal
responses in Brassicaceae

a 2025

Hairy root tissue culture as a tool for studying hormonal responses in Brassicaceae

PUKYŠOVÁ, Vendula; Veronika JEDLIČKOVÁ; Matej ZÁMEČNÍK a Helene ROBERT BOISIVON

Základní údaje

Originální název

Hairy root tissue culture as a tool for studying hormonal responses in Brassicaceae

Autoři

PUKYŠOVÁ, Vendula; Veronika JEDLIČKOVÁ; Matej ZÁMEČNÍK a Helene ROBERT BOISIVON

Vydání

Auxcentric meeting, Wageningen, Nizozemí, 2025

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10611 Plant sciences, botany

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Hairy root culture; Brassica napus; Hormone reporters; Auxin; cytokinin; signaling; Gene expression analysis

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 3. 2026 08:55, Mgr. Eva Dubská

Anotace

V originále

Hairy root (HR) tissue culture is an efficient system for studying gene function and hormone responses in Brassicaceae species, including Brassica napus and Brassica rapa. HR transformation enables rapid generation of transgenic root lines, allowing functional analysis of introduced genes. In this study, cytokinin and auxin reporter systems were used to monitor hormonal responses in HRs. A cytokinin-responsive reporter (TCSv2:3×VENUS) showed increased signal intensity after cytokinin treatment, confirming active cytokinin signaling. Additionally, auxin output reporters (DR5 and pIAA motif-based constructs) demonstrated auxin responsiveness under different treatment conditions. These systems allow visualization of hormone activity in root tissues with high spatial resolution. HR-derived plants can be regenerated and used for further genetic and functional analyses. Furthermore, the expression of TAA1 gene family members in B. napus was investigated, revealing differential expression patterns. Overall, HR tissue culture provides a versatile platform for studying hormonal regulation and gene expression in Brassicaceae.

Návaznosti

EH22_008/0004581, projekt VaV

Název: Nové poznatky pro plodiny nové generace

Citovat

PUKYŠOVÁ, Vendula; Veronika JEDLIČKOVÁ; Matej ZÁMEČNÍK a Helene ROBERT BOISIVON. Hairy root tissue culture as a tool for studying hormonal responses in Brassicaceae. In Auxcentric meeting, Wageningen, Nizozemí. 2025.

@proceedings{2561118,
   author = {Pukyšová, Vendula and Jedličková, Veronika and Zámečník, Matej and Robert Boisivon, Helene},
   booktitle = {Auxcentric meeting, Wageningen, Nizozemí},
   keywords = {Hairy root culture; Brassica napus; Hormone reporters; Auxin; cytokinin; signaling; Gene expression analysis},
   language = {eng},
   title = {Hairy root tissue culture as a tool for studying hormonal responses in Brassicaceae},
   url = {https://www.nwo.nl/en/projects/julfx97200},
   year = {2025}
}

TY  - CONF
ID  - 2561118
AU  - Pukyšová, Vendula - Jedličková, Veronika - Zámečník, Matej - Robert Boisivon, Helene
PY  - 2025
TI  - Hairy root tissue culture as a tool for studying hormonal responses in Brassicaceae
KW  - Hairy root culture
KW  - Brassica napus
KW  - Hormone reporters
KW  - Auxin
KW  - cytokinin
KW  - signaling
KW  - Gene expression analysis
UR  - https://www.nwo.nl/en/projects/julfx97200
N2  - Hairy root (HR) tissue culture is an efficient system for studying gene function and hormone responses in Brassicaceae species, including Brassica napus and Brassica rapa. HR transformation enables rapid generation of transgenic root lines, allowing functional analysis of introduced genes. In this study, cytokinin and auxin reporter systems were used to monitor hormonal responses in HRs. A cytokinin-responsive reporter (TCSv2:3×VENUS) showed increased signal intensity after cytokinin treatment, confirming active cytokinin signaling. Additionally, auxin output reporters (DR5 and pIAA motif-based constructs) demonstrated auxin responsiveness under different treatment conditions. These systems allow visualization of hormone activity in root tissues with high spatial resolution. HR-derived plants can be regenerated and used for further genetic and functional analyses. Furthermore, the expression of TAA1 gene family members in B. napus was investigated, revealing differential expression patterns. Overall, HR tissue culture provides a versatile platform for studying hormonal regulation and gene expression in Brassicaceae.
ER  -

PUKYŠOVÁ, Vendula; Veronika JEDLIČKOVÁ; Matej ZÁMEČNÍK a Helene ROBERT BOISIVON. Hairy root tissue culture as a tool for studying hormonal responses in Brassicaceae. In \textit{Auxcentric meeting, Wageningen, Nizozemí}. 2025.

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