Shining a light on viral detection: a comparative study of
electrochemical and electrochemiluminescence techniques for
human cytomegalovirus

J 2026

Shining a light on viral detection: a comparative study of electrochemical and electrochemiluminescence techniques for human cytomegalovirus

FRIED, Aneta; Karolína ITTERHEIMOVÁ; Ludmila MORÁŇOVÁ a Martin BARTOŠÍK

Základní údaje

Originální název

Shining a light on viral detection: a comparative study of electrochemical and electrochemiluminescence techniques for human cytomegalovirus

Autoři

FRIED, Aneta ; Karolína ITTERHEIMOVÁ ; Ludmila MORÁŇOVÁ a Martin BARTOŠÍK

Vydání

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Springer-Verlag, 2026, 1618-2642

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10609 Biochemical research methods

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.800 v roce 2024

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1007/s00216-026-06410-8

UT WoS

001711774300001

Klíčová slova česky

Lidský cytomegalovirus; Elektrochemiluminiscence; Elektrochemie; Biomarker rakoviny; Bioassay

Klíčová slova anglicky

Human cytomegalovirus; Electrochemiluminescence; Electrochemistry; Cancer biomarker; Bioassay

Štítky

143160

Příznaky

Recenzováno

Změněno: 19. 3. 2026 16:36, Mgr. Petra Trembecká, Ph.D.

Anotace

V originále

Human cytomegalovirus (hCMV) is a widespread herpesvirus proposed to exert oncomodulatory effects on several cancers by influencing cell proliferation, inflammation, and immune evasion. hCMV DNA and proteins have been detected in tumors, such as glioblastoma and colorectal cancer, suggesting a possible role in tumor biology. However, detection rates in clinical samples vary dramatically from 0 to 100%, reflecting both biological heterogeneity and methodological differences. Standard detection methods such as immunohistochemistry, qPCR, and sequencing provide good sensitivity, but each has limitations, including inconsistent antibody performance, costly instrumentation, and limited suitability for routine screening in point-ofcare settings. Electrochemical (EC) and electrogenerated chemiluminescence (ECL) bioassays and biosensors offer attractive alternatives, providing rapid, simple, and low-sample-consumption assays that are well suited for point-of-care testing. These platforms combine high analytical sensitivity with minimal instrumentation requirements, short turnaround times, and compatibility with amplification strategies, making them promising tools for decentralized viral diagnostics. In this study, we optimized and compared EC and ECL bioassays coupled with various amplification strategies, including PCR, recombinase polymerase amplification (RPA), and loop-mediated isothermal amplification (LAMP), for hCMV detection in real samples. Amperometric EC readout combined with PCR or RPA showed high specificity, reliably distinguishing hCMV-infected from uninfected cells, and demonstrating its potential for use in tumor tissue analysis. LAMP offers excellent sensitivity but suffers from nonspecific amplification, whereas the ECL assay failed to detect viral DNA under our conditions. These results highlight the relative strengths and limitations of these platforms and indicate their suitability for future hCMV diagnostics.

Citovat

FRIED, Aneta; Karolína ITTERHEIMOVÁ; Ludmila MORÁŇOVÁ a Martin BARTOŠÍK. Shining a light on viral detection: a comparative study of electrochemical and electrochemiluminescence techniques for human cytomegalovirus. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. Springer-Verlag, 2026, 15 s. ISSN 1618-2642. Dostupné z: https://doi.org/10.1007/s00216-026-06410-8.

@article{2561298,
   author = {Fried, Aneta and Itterheimová, Karolína and Moráňová, Ludmila and Bartošík, Martin},
   doi = {https://doi.org/10.1007/s00216-026-06410-8},
   keywords = {Human cytomegalovirus; Electrochemiluminescence; Electrochemistry; Cancer biomarker; Bioassay},
   language = {eng},
   issn = {1618-2642},
   journal = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
   title = {Shining a light on viral detection: a comparative study of electrochemical and electrochemiluminescence techniques for human cytomegalovirus},
   url = {https://link.springer.com/article/10.1007/s00216-026-06410-8#citeas},
   year = {2026}
}

TY  - JOUR
ID  - 2561298
AU  - Fried, Aneta - Itterheimová, Karolína - Moráňová, Ludmila - Bartošík, Martin
PY  - 2026
TI  - Shining a light on viral detection: a comparative study of electrochemical and electrochemiluminescence techniques for human cytomegalovirus
JF  - ANALYTICAL AND BIOANALYTICAL CHEMISTRY
PB  - Springer-Verlag
SN  - 16182642
KW  - Human cytomegalovirus
KW  - Electrochemiluminescence
KW  - Electrochemistry
KW  - Cancer biomarker
KW  - Bioassay
UR  - https://link.springer.com/article/10.1007/s00216-026-06410-8#citeas
N2  - Human cytomegalovirus (hCMV) is a widespread herpesvirus proposed to exert oncomodulatory effects on several cancers by influencing cell proliferation, inflammation, and immune evasion. hCMV DNA and proteins have been detected in tumors, such as glioblastoma and colorectal cancer, suggesting a possible role in tumor biology. However, detection rates in clinical samples vary dramatically from 0 to 100%, reflecting both biological heterogeneity and methodological differences. Standard detection methods such as immunohistochemistry, qPCR, and sequencing provide good sensitivity, but each has limitations, including inconsistent antibody performance, costly instrumentation, and limited suitability for routine screening in point-ofcare settings. Electrochemical (EC) and electrogenerated chemiluminescence (ECL) bioassays and biosensors offer attractive alternatives, providing rapid, simple, and low-sample-consumption assays that are well suited for point-of-care testing. These platforms combine high analytical sensitivity with minimal instrumentation requirements, short turnaround times, and compatibility with amplification strategies, making them promising tools for decentralized viral diagnostics. In this study, we optimized and compared EC and ECL bioassays coupled with various amplification strategies, including PCR, recombinase polymerase amplification (RPA), and loop-mediated isothermal amplification (LAMP), for hCMV detection in real samples. Amperometric EC readout combined with PCR or RPA showed high specificity, reliably distinguishing hCMV-infected from uninfected cells, and demonstrating its potential for use in tumor tissue analysis. LAMP offers excellent sensitivity but suffers from nonspecific amplification, whereas the ECL assay failed to detect viral DNA under our conditions. These results highlight the relative strengths and limitations of these platforms and indicate their suitability for future hCMV diagnostics.
ER  -

FRIED, Aneta; Karolína ITTERHEIMOVÁ; Ludmila MORÁŇOVÁ a Martin BARTOŠÍK. Shining a light on viral detection: a comparative study of electrochemical and electrochemiluminescence techniques for human cytomegalovirus. \textit{ANALYTICAL AND BIOANALYTICAL CHEMISTRY}. Springer-Verlag, 2026, 15 s. ISSN~1618-2642. Dostupné z: https://doi.org/10.1007/s00216-026-06410-8.

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