Chromosome Painting Using Chromosome-Specific BAC Clones

C 2023

Chromosome Painting Using Chromosome-Specific BAC Clones

MALÍK MANDÁKOVÁ, Terezie a Martin LYSÁK

Základní údaje

Originální název

Chromosome Painting Using Chromosome-Specific BAC Clones

Autoři

MALÍK MANDÁKOVÁ, Terezie a Martin LYSÁK

Vydání

New York, USA, Methods in Molecular Biology, od s. 303 - 313, 23 s. 2672, 2023

Nakladatel

Springer Nature, Humana Press Inc.

Další údaje

Jazyk

angličtina

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/23:00144065

Organizační jednotka

Středoevropský technologický institut

ISBN

978-1-0716-3225-3

DOI

https://doi.org/10.1007/978-1-0716-3226-0_19

EID Scopus

2-s2.0-85162725846

Klíčová slova anglicky

Arabidopsis thaliana; BAC FISH; Brassicaceae; Chromosome painting; Fluorescence in situ hybridization (FISH); Nick translation

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 4. 2026 12:31, Mgr. Eva Dubská

Anotace

V originále

Chromosome painting (CP) refers to visualization of large chromosome regions, chromosome arms or entire chromosomes via fluorescence in situ hybridization (FISH) of chromosome-specific DNA sequences. For CP in crucifers (Brassicaceae), typically contigs of chromosome-specific bacterial artificial chromosomes (BAC) from Arabidopsis thaliana are applied as painting probes on chromosomes of A. thaliana or other species (comparative chromosome painting, CCP). CP/CCP enables to identify and trace particular chromosome regions and/or chromosomes throughout all mitotic and meiotic stages as well as corresponding interphase chromosome territories. However, extended pachytene chromosomes provide the highest resolution of CP/CCP. Fine-scale chromosome structure, structural chromosome rearrangements (such as inversions, translocations, centromere repositioning), and chromosome breakpoints can be investigated by CP/CCP. BAC DNA probes can be accompanied by other types of DNA probes, such as repetitive DNA, genomic DNA, or synthetic oligonucleotide probes. Here, we describe a robust step-by-step protocol of CP and CCP which proved to be efficient across the family Brassicaceae, but which is also applicable to other angiosperm families.

Návaznosti

GA21-06839S, projekt VaV

Název: Vznik a evoluce chromozomů spojených s apomixií v rodu Boechera (Brassicaceae)

Investor: Grantová agentura ČR, The origin and evolution of apomixis-related chromosomes in Boechera (Brassicaceae)

Citovat

MALÍK MANDÁKOVÁ, Terezie a Martin LYSÁK. Chromosome Painting Using Chromosome-Specific BAC Clones. Online. In Tony Heitkam, Sònia Garcia. Methods in Molecular Biology. New York, USA: Springer Nature, Humana Press Inc., 2023, s. 303 - 313, 23 s. 2672. ISBN 978-1-0716-3225-3. Dostupné z: https://doi.org/10.1007/978-1-0716-3226-0_19.

@inbook{2563852,
   author = {Malík Mandáková, Terezie and Lysák, Martin},
   address = {New York, USA},
   booktitle = {Methods in Molecular Biology},
   doi = {https://doi.org/10.1007/978-1-0716-3226-0_19},
   editor = {Tony Heitkam, Sònia Garcia},
   keywords = {Arabidopsis thaliana; BAC FISH; Brassicaceae; Chromosome painting; Fluorescence in situ hybridization (FISH); Nick translation},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {New York, USA},
   isbn = {978-1-0716-3225-3},
   pages = {303 - 313},
   publisher = {Springer Nature, Humana Press Inc.},
   title = {Chromosome Painting Using Chromosome-Specific BAC Clones},
   url = {https://link.springer.com/protocol/10.1007/978-1-0716-3226-0_19},
   year = {2023}
}

TY  - CHAP
ID  - 2563852
AU  - Malík Mandáková, Terezie - Lysák, Martin
PY  - 2023
TI  - Chromosome Painting Using Chromosome-Specific BAC Clones
VL  - 2672
PB  - Springer Nature, Humana Press Inc.
CY  - New York, USA
SN  - 9781071632253
KW  - Arabidopsis thaliana
KW  - BAC FISH
KW  - Brassicaceae
KW  - Chromosome painting
KW  - Fluorescence in situ hybridization (FISH)
KW  - Nick translation
UR  - https://link.springer.com/protocol/10.1007/978-1-0716-3226-0_19
N2  - Chromosome painting (CP) refers to visualization of large chromosome regions, chromosome arms or entire chromosomes via fluorescence in situ hybridization (FISH) of chromosome-specific DNA sequences. For CP in crucifers (Brassicaceae), typically contigs of chromosome-specific bacterial artificial chromosomes (BAC) from Arabidopsis thaliana are applied as painting probes on chromosomes of A. thaliana or other species (comparative chromosome painting, CCP). CP/CCP enables to identify and trace particular chromosome regions and/or chromosomes throughout all mitotic and meiotic stages as well as corresponding interphase chromosome territories. However, extended pachytene chromosomes provide the highest resolution of CP/CCP. Fine-scale chromosome structure, structural chromosome rearrangements (such as inversions, translocations, centromere repositioning), and chromosome breakpoints can be investigated by CP/CCP. BAC DNA probes can be accompanied by other types of DNA probes, such as repetitive DNA, genomic DNA, or synthetic oligonucleotide probes. Here, we describe a robust step-by-step protocol of CP and CCP which proved to be efficient across the family Brassicaceae, but which is also applicable to other angiosperm families.
ER  -

MALÍK MANDÁKOVÁ, Terezie a Martin LYSÁK. Chromosome Painting Using Chromosome-Specific BAC Clones. Online. In Tony Heitkam, Sònia Garcia. \textit{Methods in Molecular Biology}. New York, USA: Springer Nature, Humana Press Inc., 2023, s.~303 - 313, 23 s. 2672. ISBN~978-1-0716-3225-3. Dostupné z: https://doi.org/10.1007/978-1-0716-3226-0\_{}19.

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