2026
Label matters: comparing gold nanoparticles and nanoshells with upconversion nanoparticles for quantitative lateral flow immunoassays
MACHÁČOVÁ, Eliška; Jakub MÁČALA; Martin KOPECKÝ; Saara KUUSINEN; Tero SOUKKA et al.Základní údaje
Originální název
Label matters: comparing gold nanoparticles and nanoshells with upconversion nanoparticles for quantitative lateral flow immunoassays
Autoři
Vydání
Microchimica Acta, Springer Nature, 2026, 0026-3672
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10406 Analytical chemistry
Stát vydavatele
Německo
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 5.300 v roce 2024
Označené pro přenos do RIV
Ne
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
Lateral flow immunoassay; Biomarker detection; Gold nanoparticle; Photon-upconversion nanoparticle; Human serum albumin; Prostate-specific antigen
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 1. 4. 2026 10:54, doc. Mgr. Zdeněk Farka, Ph.D.
Anotace
V originále
Lateral flow immunoassay (LFIA) is a widely used point-of-care method for both qualitative and semi- or fully quantitative detection of clinical biomarkers. There has, however, been limited data available on direct comparison of different types of labels in the LFIA for quantitative analysis using the same non-competitive assay setup. In this study, two groups of antibody-conjugated nanoparticle labels are compared: conventional gold nanostructures with densitometry readout and upconversion nanoparticles (UCNPs) suitable for sensitive luminescence readout. First, the LFIA procedure was optimized using human serum albumin (HSA) as an analyte. In the group of gold-based labels, standard gold nanoparticles were compared with gold nanoshells (AuNSs). In the case of UCNP labels, doping with Er3+ and Tm3+ and surface modifications based on poly(ethylene glycol) and poly(acrylic acid) (PAA) were compared. The best representatives—AuNSs and PAA-Tm UCNPs—were successfully used to detect human serum albumin (HSA) in spiked urine samples. To assess assay versatility, prostate-specific antigen (PSA) was detected in serum. While AuNSs tended to aggregate in serum, the PAA-Tm UCNPs maintained their stability and achieved the limit of detection of 34 pg/mL. Finally, spiked serum samples were analyzed to evaluate detection trueness, achieving a strong correlation with the reference concentrations (recoveries ranging from 82 to 118%). We showed that both label choice and sample matrix are critical: while AuNSs and PAA-Tm UCNPs performed well in urine, PAA-Tm UCNPs excelled in the more complex serum matrix. Thus, PAA-Tm UCNPs were found to be the most suitable labels for biomarker quantitation, highlighting the strong potential of UCNPs for sensitive LFIA applications.
Návaznosti
| CZ.02.01.01/00/23_015/0008175, interní kód MU |
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| LM2023042, projekt VaV |
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| LUC24105, projekt VaV |
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| MUNI/C/0091/2025, interní kód MU |
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| MUNI/G/1125/2022, interní kód MU |
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