Clonal relatedness and emerging linezolid resistance in
Clostridioides difficile ribotypes 001 and 176 in the Czech
Republic

J 2026

Clonal relatedness and emerging linezolid resistance in Clostridioides difficile ribotypes 001 and 176 in the Czech Republic

BRAJEROVA, Marie; Pete KINROSS; Elka NYCOVA; Ivana VITKOVA; Magda BALEJOVA et al.

Základní údaje

Originální název

Clonal relatedness and emerging linezolid resistance in Clostridioides difficile ribotypes 001 and 176 in the Czech Republic

Autoři

Vydání

International journal of infectious diseases, London, ELSEVIER SCI LTD, 2026, 1201-9712

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.300 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Antimicrobial resistance; Antimicrobial use; Whole-genome sequencing; Nanopore; cfrE; cfrB

Štítky

14110113, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 4. 2026 12:32, Mgr. Tereza Miškechová

Anotace

V originále

Background and aims: Clostridioides difficile ribotypes (RTs) 001 and 176 dominate the epidemiology of C. difficile infection (CDI) in the Czech Republic. We applied whole-genome sequencing (WGS) and broad-range antimicrobial susceptibility testing (AST) to provide detailed characterization of these lineages. Methods: Between October and December 2019, 22 hospitals participated. CDI isolates were characterized by PCR ribotyping, toxin gene detection and AST to metronidazole, vancomycin and moxifloxacin. WGS and broad-range AST were performed on selected RT001 and RT176 isolates. Results: The mean CDI incidence was 5.1 cases/10,000 patient-days. Isolates and epidemiological data were available for 495/524 (94.5%) CDIs. RT001 ( n = 166, 33.5%), RT014 ( n = 59, 11.9%) RT176 ( n = 51, 10.3%) were the most prevalent. Among 21 RT001 isolates, wgMLST (3745 loci) revealed 0-120 allele differences and 21 genomic inserts, eight of which carried antimicrobial resistance (AMR) genes, including cfrB, which encodes linezolid resistance. In 13 RT176 isolates, wgMLST (3298 loci) showed 0-9 allele differences and 11 inserts, eight with AMR genes. The wgMLST confirmed clonal relatedness of RT001 and RT176 isolates from different hospitals (0-3 allelic differences), yet with variation in acquired AMR gene content. Differences between genotype and expected phenotype were observed in PnimBG and metronidazole, cfrE and linezolid and tetgenes and tetracycline. Conclusion: RT001 and RT176 predominate in the Czech Republic, and WGS confirmed their inter-hospital clonal relatedness. Importantly, the emergence of linezolid-resistant RT001 strains was detected in nine hospitals. The inclusion of AMR genes in genetic relatedness analysis showed higher discriminatory power compared to cgMLST or wgMLST alone. (c) 2026 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)

Návaznosti

LX22NPO5103, projekt VaV

Název: Národní institut virologie a bakteriologie (Akronym: NIVB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní institut virologie a bakteriologie, 5.1 EXCELES

Citovat

BRAJEROVA, Marie; Pete KINROSS; Elka NYCOVA; Ivana VITKOVA; Magda BALEJOVA; Lenka GEIGEROVA; Natasa BARTONIKOVA; Renata TEJKALOVÁ; Lenka HAVLINOVA; Iva VAGNEROVA; Zuzana KADLECKOVA; Lenka RYSKOVA; Petr JEZEK; Dana ZAMAZALOVA; Denisa VESELA; Alice KUCHAROVA; Marketa SKRUZNA; Martina CURDOVA; Antonin MELICHAR; Eva SIMECKOVA; Erika CZYZOVA; Eliska MISKOVA; Tereza PROKOPOVA; Otakar NYC; Pavel DREVINEK a Marcela KRUTOVA. Clonal relatedness and emerging linezolid resistance in Clostridioides difficile ribotypes 001 and 176 in the Czech Republic. International journal of infectious diseases. London: ELSEVIER SCI LTD, 2026, roč. 165, April 2026, s. 1-9. ISSN 1201-9712. Dostupné z: https://doi.org/10.1016/j.ijid.2026.108444.

@article{2565339,
   author = {Brajerova, Marie and Kinross, Pete and Nycova, Elka and Vitkova, Ivana and Balejova, Magda and Geigerova, Lenka and Bartonikova, Natasa and Tejkalová, Renata and Havlinova, Lenka and Vagnerova, Iva and Kadleckova, Zuzana and Ryskova, Lenka and Jezek, Petr and Zamazalova, Dana and Vesela, Denisa and Kucharova, Alice and Skruzna, Marketa and Curdova, Martina and Melichar, Antonin and Simeckova, Eva and Czyzova, Erika and Miskova, Eliska and Prokopova, Tereza and Nyc, Otakar and Drevinek, Pavel and Krutova, Marcela},
   article_location = {London},
   article_number = {April 2026},
   doi = {https://doi.org/10.1016/j.ijid.2026.108444},
   keywords = {Antimicrobial resistance; Antimicrobial use; Whole-genome sequencing; Nanopore; cfrE; cfrB},
   language = {eng},
   issn = {1201-9712},
   journal = {International journal of infectious diseases},
   title = {Clonal relatedness and emerging linezolid resistance in Clostridioides difficile ribotypes 001 and 176 in the Czech Republic},
   url = {https://www.sciencedirect.com/science/article/pii/S1201971226000792?via%3Dihub},
   volume = {165},
   year = {2026}
}

TY  - JOUR
ID  - 2565339
AU  - Brajerova, Marie - Kinross, Pete - Nycova, Elka - Vitkova, Ivana - Balejova, Magda - Geigerova, Lenka - Bartonikova, Natasa - Tejkalová, Renata - Havlinova, Lenka - Vagnerova, Iva - Kadleckova, Zuzana - Ryskova, Lenka - Jezek, Petr - Zamazalova, Dana - Vesela, Denisa - Kucharova, Alice - Skruzna, Marketa - Curdova, Martina - Melichar, Antonin - Simeckova, Eva - Czyzova, Erika - Miskova, Eliska - Prokopova, Tereza - Nyc, Otakar - Drevinek, Pavel - Krutova, Marcela
PY  - 2026
TI  - Clonal relatedness and emerging linezolid resistance in Clostridioides difficile ribotypes 001 and 176 in the Czech Republic
JF  - International journal of infectious diseases
VL  - 165
IS  - April 2026
SP  - 1-9
EP  - 1-9
PB  - ELSEVIER SCI LTD
SN  - 12019712
KW  - Antimicrobial resistance
KW  - Antimicrobial use
KW  - Whole-genome sequencing
KW  - Nanopore
KW  - cfrE
KW  - cfrB
UR  - https://www.sciencedirect.com/science/article/pii/S1201971226000792?via%3Dihub
N2  - Background and aims: Clostridioides difficile ribotypes (RTs) 001 and 176 dominate the epidemiology of C. difficile infection (CDI) in the Czech Republic. We applied whole-genome sequencing (WGS) and broad-range antimicrobial susceptibility testing (AST) to provide detailed characterization of these lineages. Methods: Between October and December 2019, 22 hospitals participated. CDI isolates were characterized by PCR ribotyping, toxin gene detection and AST to metronidazole, vancomycin and moxifloxacin. WGS and broad-range AST were performed on selected RT001 and RT176 isolates. Results: The mean CDI incidence was 5.1 cases/10,000 patient-days. Isolates and epidemiological data were available for 495/524 (94.5%) CDIs. RT001 ( n = 166, 33.5%), RT014 ( n = 59, 11.9%) RT176 ( n = 51, 10.3%) were the most prevalent. Among 21 RT001 isolates, wgMLST (3745 loci) revealed 0-120 allele differences and 21 genomic inserts, eight of which carried antimicrobial resistance (AMR) genes, including cfrB, which encodes linezolid resistance. In 13 RT176 isolates, wgMLST (3298 loci) showed 0-9 allele differences and 11 inserts, eight with AMR genes. The wgMLST confirmed clonal relatedness of RT001 and RT176 isolates from different hospitals (0-3 allelic differences), yet with variation in acquired AMR gene content. Differences between genotype and expected phenotype were observed in PnimBG and metronidazole, cfrE and linezolid and tetgenes and tetracycline. Conclusion: RT001 and RT176 predominate in the Czech Republic, and WGS confirmed their inter-hospital clonal relatedness. Importantly, the emergence of linezolid-resistant RT001 strains was detected in nine hospitals. The inclusion of AMR genes in genetic relatedness analysis showed higher discriminatory power compared to cgMLST or wgMLST alone. (c) 2026 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
ER  -

BRAJEROVA, Marie; Pete KINROSS; Elka NYCOVA; Ivana VITKOVA; Magda BALEJOVA; Lenka GEIGEROVA; Natasa BARTONIKOVA; Renata TEJKALOVÁ; Lenka HAVLINOVA; Iva VAGNEROVA; Zuzana KADLECKOVA; Lenka RYSKOVA; Petr JEZEK; Dana ZAMAZALOVA; Denisa VESELA; Alice KUCHAROVA; Marketa SKRUZNA; Martina CURDOVA; Antonin MELICHAR; Eva SIMECKOVA; Erika CZYZOVA; Eliska MISKOVA; Tereza PROKOPOVA; Otakar NYC; Pavel DREVINEK a Marcela KRUTOVA. Clonal relatedness and emerging linezolid resistance in Clostridioides difficile ribotypes 001 and 176 in the Czech Republic. \textit{International journal of infectious diseases}. London: ELSEVIER SCI LTD, 2026, roč.~165, April 2026, s.~1-9. ISSN~1201-9712. Dostupné z: https://doi.org/10.1016/j.ijid.2026.108444.

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