Development of in vitro cultures from Astragalus thracicus
Griseb and Astragalus aitosensis (Ivanisch.) and evaluation of
their bioproduction potential

J 2026

Development of in vitro cultures from Astragalus thracicus Griseb and Astragalus aitosensis (Ivanisch.) and evaluation of their bioproduction potential

VASILEV, Hristo; Karel ŠMEJKAL; Jakub TREML; Christian Schulze GRONOVER; Dirk PRUFER et al.

Základní údaje

Originální název

Development of in vitro cultures from Astragalus thracicus Griseb and Astragalus aitosensis (Ivanisch.) and evaluation of their bioproduction potential

Autoři

VASILEV, Hristo; Karel ŠMEJKAL; Jakub TREML; Christian Schulze GRONOVER a Dirk PRUFER

Vydání

PLANT CELL TISSUE AND ORGAN CULTURE, DORDRECHT, SPRINGER, 2026, 0167-6857

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Nizozemské království

Odkazy

URL

Impakt faktor

Impact factor: 2.400 v roce 2024

Označené pro přenos do RIV

Organizační jednotka

Farmaceutická fakulta

Klíčová slova anglicky

Astragalus species; callus cultures; Elicitation; Flavonoid; Stress-induction

Štítky

ÚMF, ÚPL

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 4. 2026 16:09, Mgr. Irena Doubková

Anotace

V originále

Two endemic Astragalus species - A. thracicus and A. aitosensis were investigated to establish and develop their in vitro cultures. We managed to optimize the in vitro growth conditions, suitable for growing and maintaining stable and long-lasting callus cultures. Furthermore, we measured the behavior and flavonoid biosynthetic potential of callus cultures after stress induction with selected elicitors: methyl jasmonate (MJ), salicylic acid (SA), and yeast extract (YE). In case of applied stress, we concluded that growth index of callus cultures from A. thracicus decreased inversely proportional to the concentrations of applied elicitors, as SA showed the strongest growth inhibiting effect. The biosynthetic potential of the same callus cultures was evaluated based on HPLC analysis of levels of three flavonol aglycons - kaempferol, quercetin, and methylated quercetin. The highest amount of kaempferol (9.12 & micro;g/g dry weight) and quercetin (5.72 & micro;g/g dry weight) were detected when callus cultures were exposed to YE at a concentration of 50 mg/l in medium for 72 hours. For the synthesis of the third studied aglycon, methylated quercetin, the best inductive conditions (equivalent to 4.69 & micro;g/g dry weight) were measured in presence of MJ at a concentration of 200 & micro;mol for 72 hours. The fastest kaempferol synthesis was found in the medium supplemented with 1000 & micro;mol/l SA at 24 hours, quantified as 7.61 & micro;g of kaempferol per gram of dry weight.

Návaznosti

GA23-04655S, projekt VaV

Název: Role prenylace a glykosylace v protizánětlivé aktivitě a metabolismu přírodních fenolových látek

Investor: Grantová agentura ČR, Role prenylace a glykosylace v protizánětlivé aktivitě a metabolismu přírodních fenolových látek

Citovat

VASILEV, Hristo; Karel ŠMEJKAL; Jakub TREML; Christian Schulze GRONOVER a Dirk PRUFER. Development of in vitro cultures from Astragalus thracicus Griseb and Astragalus aitosensis (Ivanisch.) and evaluation of their bioproduction potential. PLANT CELL TISSUE AND ORGAN CULTURE. DORDRECHT: SPRINGER, 2026, roč. 164, č. 3, s. 1-15. ISSN 0167-6857. Dostupné z: https://doi.org/10.1007/s11240-026-03395-1.

@article{2565499,
   author = {Vasilev, Hristo and Šmejkal, Karel and Treml, Jakub and Gronover, Christian Schulze and Prufer, Dirk},
   article_location = {DORDRECHT},
   article_number = {3},
   doi = {https://doi.org/10.1007/s11240-026-03395-1},
   keywords = {Astragalus species; callus cultures; Elicitation; Flavonoid; Stress-induction},
   language = {eng},
   issn = {0167-6857},
   journal = {PLANT CELL TISSUE AND ORGAN CULTURE},
   title = {Development of in vitro cultures from Astragalus thracicus Griseb and Astragalus aitosensis (Ivanisch.) and evaluation of their bioproduction potential},
   url = {https://link.springer.com/article/10.1007/s11240-026-03395-1#Ack1},
   volume = {164},
   year = {2026}
}

TY  - JOUR
ID  - 2565499
AU  - Vasilev, Hristo - Šmejkal, Karel - Treml, Jakub - Gronover, Christian Schulze - Prufer, Dirk
PY  - 2026
TI  - Development of in vitro cultures from Astragalus thracicus Griseb and Astragalus aitosensis (Ivanisch.) and evaluation of their bioproduction potential
JF  - PLANT CELL TISSUE AND ORGAN CULTURE
VL  - 164
IS  - 3
SP  - 1-15
EP  - 1-15
PB  - SPRINGER
SN  - 01676857
KW  - Astragalus species
KW  - callus cultures
KW  - Elicitation
KW  - Flavonoid
KW  - Stress-induction
UR  - https://link.springer.com/article/10.1007/s11240-026-03395-1#Ack1
N2  - Two endemic Astragalus species - A. thracicus and A. aitosensis were investigated to establish and develop their in vitro cultures. We managed to optimize the in vitro growth conditions, suitable for growing and maintaining stable and long-lasting callus cultures. Furthermore, we measured the behavior and flavonoid biosynthetic potential of callus cultures after stress induction with selected elicitors: methyl jasmonate (MJ), salicylic acid (SA), and yeast extract (YE). In case of applied stress, we concluded that growth index of callus cultures from A. thracicus decreased inversely proportional to the concentrations of applied elicitors, as SA showed the strongest growth inhibiting effect. The biosynthetic potential of the same callus cultures was evaluated based on HPLC analysis of levels of three flavonol aglycons - kaempferol, quercetin, and methylated quercetin. The highest amount of kaempferol (9.12 & micro;g/g dry weight) and quercetin (5.72 & micro;g/g dry weight) were detected when callus cultures were exposed to YE at a concentration of 50 mg/l in medium for 72 hours. For the synthesis of the third studied aglycon, methylated quercetin, the best inductive conditions (equivalent to 4.69 & micro;g/g dry weight) were measured in presence of MJ at a concentration of 200 & micro;mol for 72 hours. The fastest kaempferol synthesis was found in the medium supplemented with 1000 & micro;mol/l SA at 24 hours, quantified as 7.61 & micro;g of kaempferol per gram of dry weight.
ER  -

VASILEV, Hristo; Karel ŠMEJKAL; Jakub TREML; Christian Schulze GRONOVER a Dirk PRUFER. Development of in vitro cultures from Astragalus thracicus Griseb and Astragalus aitosensis (Ivanisch.) and evaluation of their bioproduction potential. \textit{PLANT CELL TISSUE AND ORGAN CULTURE}. DORDRECHT: SPRINGER, 2026, roč.~164, č.~3, s.~1-15. ISSN~0167-6857. Dostupné z: https://doi.org/10.1007/s11240-026-03395-1.

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