Nucleoli-localized KANSL2 as an epigenetic regulator of
ribosome biogenesis in glioblastoma cells

J 2026

Nucleoli-localized KANSL2 as an epigenetic regulator of ribosome biogenesis in glioblastoma cells

BUDNIK, Nicolas; Lucia CANEDO; Agustin E MORELLATO; Marina B CUENCA; Martina GARMENDIA et al.

Základní údaje

Originální název

Nucleoli-localized KANSL2 as an epigenetic regulator of ribosome biogenesis in glioblastoma cells

Autoři

Vydání

Communications Biology, BERLIN, Nature Research, 2026, 2399-3642

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.100 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

KANSL2; nucleolus; ribosome biogenesis; epigenetic regulation; glioblastoma

Štítky

14110513, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 5. 2026 13:56, Mgr. Tereza Miškechová

Anotace

V originále

KANSL2 is a subunit of the non-specific lethal (NSL) chromatin-modifying complex associated with glioblastoma (GBM) progression, but the intrinsic role of KANSL2 in GBM cells is poorly understood. By analyzing TCGA-GBM and GTEx datasets, we found that KANSL2 is upregulated in GBM and positively correlates with genes involved in ribosome biogenesis. Immunofluorescence and cell cycle analyses reveal a dynamic nuclear distribution, with KANSL2 becoming enriched in nucleoli mainly during G1/early S and G2 phases. Overexpression of KANSL2 increases 45S pre-rRNA and 28S rRNA levels, whereas its silencing reduces rRNA expression and histone H4 acetylation at lysines 5 and 8 within rDNA promoters. RNA-seq of patient-derived GBM spheroids confirms a global downregulation of ribosome biogenesis genes upon silencing of KANSL2. Together, these findings identify KANSL2 as a nuclear factor that transiently associates with nucleoli to promote rRNA transcription and ribosome biogenesis, supporting the biosynthetic and proliferative capacity of glioblastoma cells.

Citovat

BUDNIK, Nicolas; Lucia CANEDO; Agustin E MORELLATO; Marina B CUENCA; Martina GARMENDIA; Sergio SENIN; Sebastian A ROMANO; Zdeněk ANDRYSÍK; Guillermo A VIDELA-RICHARDSON; Michael W GRANER; Ken KOBAYASHI; Yilong ZHOU; Meike WIESE; Asifa AKHTAR; Joaquin M ESPINOSA a Carolina PEREZ-CASTRO. Nucleoli-localized KANSL2 as an epigenetic regulator of ribosome biogenesis in glioblastoma cells. Communications Biology. BERLIN: Nature Research, 2026, roč. 9, č. 1, s. 1-14. ISSN 2399-3642. Dostupné z: https://doi.org/10.1038/s42003-026-09808-3.

@article{2570604,
   author = {Budnik, Nicolas and Canedo, Lucia and Morellato, Agustin E and Cuenca, Marina B and Garmendia, Martina and Senin, Sergio and Romano, Sebastian A and Andrysík, Zdeněk and VidelaandRichardson, Guillermo A and Graner, Michael W and Kobayashi, Ken and Zhou, Yilong and Wiese, Meike and Akhtar, Asifa and Espinosa, Joaquin M and PerezandCastro, Carolina},
   article_location = {BERLIN},
   article_number = {1},
   doi = {https://doi.org/10.1038/s42003-026-09808-3},
   keywords = {KANSL2; nucleolus; ribosome biogenesis; epigenetic regulation; glioblastoma},
   language = {eng},
   issn = {2399-3642},
   journal = {Communications Biology},
   title = {Nucleoli-localized KANSL2 as an epigenetic regulator of ribosome biogenesis in glioblastoma cells},
   url = {https://www.nature.com/articles/s42003-026-09808-3},
   volume = {9},
   year = {2026}
}

TY  - JOUR
ID  - 2570604
AU  - Budnik, Nicolas - Canedo, Lucia - Morellato, Agustin E - Cuenca, Marina B - Garmendia, Martina - Senin, Sergio - Romano, Sebastian A - Andrysík, Zdeněk - Videla-Richardson, Guillermo A - Graner, Michael W - Kobayashi, Ken - Zhou, Yilong - Wiese, Meike - Akhtar, Asifa - Espinosa, Joaquin M - Perez-Castro, Carolina
PY  - 2026
TI  - Nucleoli-localized KANSL2 as an epigenetic regulator of ribosome biogenesis in glioblastoma cells
JF  - Communications Biology
VL  - 9
IS  - 1
SP  - 1-14
EP  - 1-14
PB  - Nature Research
SN  - 23993642
KW  - KANSL2
KW  - nucleolus
KW  - ribosome biogenesis
KW  - epigenetic regulation
KW  - glioblastoma
UR  - https://www.nature.com/articles/s42003-026-09808-3
N2  - KANSL2 is a subunit of the non-specific lethal (NSL) chromatin-modifying complex associated with glioblastoma (GBM) progression, but the intrinsic role of KANSL2 in GBM cells is poorly understood. By analyzing TCGA-GBM and GTEx datasets, we found that KANSL2 is upregulated in GBM and positively correlates with genes involved in ribosome biogenesis. Immunofluorescence and cell cycle analyses reveal a dynamic nuclear distribution, with KANSL2 becoming enriched in nucleoli mainly during G1/early S and G2 phases. Overexpression of KANSL2 increases 45S pre-rRNA and 28S rRNA levels, whereas its silencing reduces rRNA expression and histone H4 acetylation at lysines 5 and 8 within rDNA promoters. RNA-seq of patient-derived GBM spheroids confirms a global downregulation of ribosome biogenesis genes upon silencing of KANSL2. Together, these findings identify KANSL2 as a nuclear factor that transiently associates with nucleoli to promote rRNA transcription and ribosome biogenesis, supporting the biosynthetic and proliferative capacity of glioblastoma cells.
ER  -

BUDNIK, Nicolas; Lucia CANEDO; Agustin E MORELLATO; Marina B CUENCA; Martina GARMENDIA; Sergio SENIN; Sebastian A ROMANO; Zdeněk ANDRYSÍK; Guillermo A VIDELA-RICHARDSON; Michael W GRANER; Ken KOBAYASHI; Yilong ZHOU; Meike WIESE; Asifa AKHTAR; Joaquin M ESPINOSA a Carolina PEREZ-CASTRO. Nucleoli-localized KANSL2 as an epigenetic regulator of ribosome biogenesis in glioblastoma cells. \textit{Communications Biology}. BERLIN: Nature Research, 2026, roč.~9, č.~1, s.~1-14. ISSN~2399-3642. Dostupné z: https://doi.org/10.1038/s42003-026-09808-3.

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