2000
Schwann cells migrating in absence of the axons in the basal lamina tubes express immunoreactivity for laminins, fibronectin, thrombospondin, and chondroitin sulfate
DUBOVÝ, Petr, Ivana SVÍŽENSKÁ a Ilona KLUSÁKOVÁZákladní údaje
Originální název
Schwann cells migrating in absence of the axons in the basal lamina tubes express immunoreactivity for laminins, fibronectin, thrombospondin, and chondroitin sulfate
Název česky
Migrující Schwannovy buňky při absenci axonů vykazují reakci na laminin, fibronectin, thrombospondin a chondroitin sulfat
Autoři
DUBOVÝ, Petr (203 Česká republika, garant), Ivana SVÍŽENSKÁ (203 Česká republika) a Ilona KLUSÁKOVÁ (203 Česká republika)
Vydání
Eur. J. Neurosci. Oxford, Blackwell Science Ltd. 2000, 0953-816X
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30000 3. Medical and Health Sciences
Stát vydavatele
Velká Británie a Severní Irsko
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 3.862
Kód RIV
RIV/00216224:14110/00:00002251
Organizační jednotka
Lékařská fakulta
UT WoS
000088236601655
Klíčová slova anglicky
ECM; migration; laminin
Změněno: 17. 6. 2009 11:50, prof. RNDr. Petr Dubový, CSc.
V originále
The acellular nerve segment is thought to be a method of choice for reparation of nerve defect. Repeated periods of freezing and thawing are frequently used for preparation of standard acellular nerve segment in experimental application. It was believed that the basal lamina tubes and endoneurial extracellular matrix molecules survive the procedures and promote the growth of regenerating axons. We used the model of freeze-treated segment of the distal nerve stump to study a molecular content of the endoneurium in absence and presence of Schwann cells. The nerve segment, marked out at the end of distal stump of transected rat sciatic nerve, was repeatedly frozen and thawed (5 times) to evacuate all resident cells. Serial longitudinal frozen sections, 10 microm thick, were incubated with monoclonal antibodies against laminin-1, -2, and -3, fibronectin, thrombospondin, as well as chondroitin sulfate. The position of Schwann cells was detected in subsequent sections or simultaneously by immunofluorescence staining with polyclonal antibody against S-100 protein. The results demonstrated that migrating Schwann cells (S-100+) repopulated the nerve segment from untreated regions of the distal nerve stump. Subsequent sections incubated for the above-mentioned molecules of the extracellular matrix revealed immunostaining only in the regions containing the Schwann cells. Simultaneous (double) immunostaining confirmed that the immunofluorescence for laminins, fibronectin, thrombospondin and chondroitin sulfate is restricted to the presence of Schwann cells. Our results suggest that Schwann cells that are crucial for arrangement of axon-promoting condition synthesize the ECM molecules and support the endoneurium by them without presence of the axons. This supports a critical role of Schwann cell migration for the growth of axons through the acellular nerve segment.
Česky
Migrující Schwanovy buňky bez přítomnosti axonů vykazují imunohistochemickou reakci na laminin, fbronectin, thrombospondin a chondroitin sulfát.
Návaznosti
GA309/00/0407, projekt VaV |
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