Schwann cells migrating in absence of the axons in the basal
lamina tubes express immunoreactivity for laminins,
fibronectin, thrombospondin, and chondroitin sulfate

J 2000

Schwann cells migrating in absence of the axons in the basal lamina tubes express immunoreactivity for laminins, fibronectin, thrombospondin, and chondroitin sulfate

DUBOVÝ, Petr, Ivana SVÍŽENSKÁ and Ilona KLUSÁKOVÁ

Basic information

Original name

Schwann cells migrating in absence of the axons in the basal lamina tubes express immunoreactivity for laminins, fibronectin, thrombospondin, and chondroitin sulfate

Name in Czech

Migrující Schwannovy buňky při absenci axonů vykazují reakci na laminin, fibronectin, thrombospondin a chondroitin sulfat

Authors

DUBOVÝ, Petr (203 Czech Republic, guarantor), Ivana SVÍŽENSKÁ (203 Czech Republic) and Ilona KLUSÁKOVÁ (203 Czech Republic)

Edition

Eur. J. Neurosci. Oxford, Blackwell Science Ltd. 2000, 0953-816X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30000 3. Medical and Health Sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.862

RIV identification code

RIV/00216224:14110/00:00002251

Organization unit

Faculty of Medicine

UT WoS

000088236601655

Keywords in English

ECM; migration; laminin

Abstract

ORIG CZ

V originále

The acellular nerve segment is thought to be a method of choice for reparation of nerve defect. Repeated periods of freezing and thawing are frequently used for preparation of standard acellular nerve segment in experimental application. It was believed that the basal lamina tubes and endoneurial extracellular matrix molecules survive the procedures and promote the growth of regenerating axons. We used the model of freeze-treated segment of the distal nerve stump to study a molecular content of the endoneurium in absence and presence of Schwann cells. The nerve segment, marked out at the end of distal stump of transected rat sciatic nerve, was repeatedly frozen and thawed (5 times) to evacuate all resident cells. Serial longitudinal frozen sections, 10 microm thick, were incubated with monoclonal antibodies against laminin-1, -2, and -3, fibronectin, thrombospondin, as well as chondroitin sulfate. The position of Schwann cells was detected in subsequent sections or simultaneously by immunofluorescence staining with polyclonal antibody against S-100 protein. The results demonstrated that migrating Schwann cells (S-100+) repopulated the nerve segment from untreated regions of the distal nerve stump. Subsequent sections incubated for the above-mentioned molecules of the extracellular matrix revealed immunostaining only in the regions containing the Schwann cells. Simultaneous (double) immunostaining confirmed that the immunofluorescence for laminins, fibronectin, thrombospondin and chondroitin sulfate is restricted to the presence of Schwann cells. Our results suggest that Schwann cells that are crucial for arrangement of axon-promoting condition synthesize the ECM molecules and support the endoneurium by them without presence of the axons. This supports a critical role of Schwann cell migration for the growth of axons through the acellular nerve segment.

In Czech

Migrující Schwanovy buňky bez přítomnosti axonů vykazují imunohistochemickou reakci na laminin, fbronectin, thrombospondin a chondroitin sulfát.

Links

GA309/00/0407, research and development project

Name: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Investor: Czech Science Foundation, Cellular and extracellular environment for the axon regenaration following injury of the spinal roots and peripheral nerves

Citovat

DUBOVÝ, Petr, Ivana SVÍŽENSKÁ and Ilona KLUSÁKOVÁ. Schwann cells migrating in absence of the axons in the basal lamina tubes express immunoreactivity for laminins, fibronectin, thrombospondin, and chondroitin sulfate. Eur. J. Neurosci. Oxford: Blackwell Science Ltd., 2000, vol. 12, Suppl. 11, p. 289. ISSN 0953-816X.

@article{323471,
   author = {Dubový, Petr and Svíženská, Ivana and Klusáková, Ilona},
   article_location = {Oxford},
   article_number = {Suppl. 11},
   keywords = {ECM; migration; laminin},
   language = {eng},
   issn = {0953-816X},
   journal = {Eur. J. Neurosci.},
   title = {Schwann cells migrating in absence of the axons in the basal lamina tubes express immunoreactivity for laminins, fibronectin, thrombospondin, and chondroitin sulfate},
   volume = {12},
   year = {2000}
}

TY  - JOUR
ID  - 323471
AU  - Dubový, Petr - Svíženská, Ivana - Klusáková, Ilona
PY  - 2000
TI  - Schwann cells migrating in absence of the axons in the basal lamina tubes express immunoreactivity for laminins, fibronectin, thrombospondin, and chondroitin sulfate
JF  - Eur. J. Neurosci.
VL  - 12
IS  - Suppl. 11
SP  - 289
EP  - 289
PB  - Blackwell Science Ltd.
SN  - 0953816X
KW  - ECM
KW  - migration
KW  - laminin
N2  - The acellular nerve segment is thought to be a method of choice for reparation of nerve defect. Repeated periods of freezing and thawing are frequently used for preparation of standard acellular nerve segment in experimental application. It was believed that the basal lamina tubes and endoneurial extracellular matrix molecules survive the procedures and promote the growth of regenerating axons. We used the model of freeze-treated segment of the distal nerve stump to study a molecular content of the endoneurium in absence and presence of Schwann cells. The nerve segment, marked out at the end of distal stump of transected rat sciatic nerve, was repeatedly frozen and thawed (5 times) to evacuate all resident cells. Serial longitudinal frozen sections, 10 microm thick, were incubated with monoclonal antibodies against laminin-1, -2, and -3, fibronectin, thrombospondin, as well as chondroitin sulfate. The position of Schwann cells was detected in subsequent sections or simultaneously by immunofluorescence staining with polyclonal antibody against S-100 protein. The results demonstrated that migrating Schwann cells (S-100+) repopulated the nerve segment from untreated regions of the distal nerve stump. Subsequent sections incubated for the above-mentioned molecules of the extracellular matrix revealed immunostaining only in the regions containing the Schwann cells. Simultaneous (double) immunostaining confirmed that the immunofluorescence for laminins, fibronectin, thrombospondin and chondroitin sulfate is restricted to the presence of Schwann cells. Our results suggest that Schwann cells that are crucial for arrangement of axon-promoting condition synthesize the ECM molecules and support the endoneurium by them without presence of the axons. This supports a critical role of Schwann cell migration for the growth of axons through the acellular nerve segment.
ER  -

DUBOVÝ, Petr, Ivana SVÍŽENSKÁ and Ilona KLUSÁKOVÁ. Schwann cells migrating in absence of the axons in the basal lamina tubes express immunoreactivity for laminins, fibronectin, thrombospondin, and chondroitin sulfate. \textit{Eur. J. Neurosci.}. Oxford: Blackwell Science Ltd., 2000, vol.~12, Suppl. 11, p.~289. ISSN~0953-816X.

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