Determination of Gentiopicroside in Extracts of Centaurium
erythreae and Gentiana lutea by Micellar Electrokinetic
Capillary Chromatography

J 2000

Determination of Gentiopicroside in Extracts of Centaurium erythreae and Gentiana lutea by Micellar Electrokinetic Capillary Chromatography

GLATZ, Zdeněk; Jiřina POSPÍŠILOVÁ a Pavel MUSIL

Základní údaje

Originální název

Determination of Gentiopicroside in Extracts of Centaurium erythreae and Gentiana lutea by Micellar Electrokinetic Capillary Chromatography

Autoři

GLATZ, Zdeněk; Jiřina POSPÍŠILOVÁ a Pavel MUSIL

Vydání

J.Liquid Chromatogr. Rel. Technol. New York, Marcel Dekker, 2000, 1082-6076

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.844

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/00:00003302

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000088408400004

Klíčová slova anglicky

micellar electrokinetic chromatography; gentiopicroside; gentiana; centaurium

Štítky

centaurium, gentiana, Gentiopicroside, Micellar Electrokinetic Chromatography

Změněno: 27. 2. 2001 10:58, prof. RNDr. Zdeněk Glatz, CSc.

Anotace

V originále

Micellar electrokinetic capillary chromatography has been developed as a promising method for the determination of gentiopicroside. The separation conditions have been optimised with respect to the different parameters including SDS concentration, pH and ion strength of the background electrolyte, and temperature of capillary. A buffer consisting of 100 mM SDS in 20 mM sodium dihydrogen phosphate, 20 mM disodium tetraborate pH 8.6 was found to be the most suitable electrolyte for this separation. The applied voltage of 28 kV (positive polarity) and the temperature of capillary 20 oC gave the best separation of secoiridiods. The linear detection range for concentration versus peak area for the assay is from 5 to 500 mg . ml-1 (correlation coefficient 0.9998) with a detection limit of 0.3 mg . ml-1. The inter-day reproducibility of the peak area was 2.5 % and the inter-day reproducibility of the migration time was below 0.16 %. The developed method was successfully used to quantify gentiopicroside in extracts of the overground parts of Centaurium erythreae and the roots of Gentiana lutea, and in the liqueur Becherovka. The results were in good agreement with those determined by HPLC.

Návaznosti

GA303/00/D062, projekt VaV

Název: Biologická dostupnost a mechanismus antioxidačního účinku fenolických kyselin a lignanů

Investor: Grantová agentura ČR, Biologická dostupnost a mechanismus antioxidačního účinku fenolických kyselin a lignanů

Citovat

GLATZ, Zdeněk; Jiřina POSPÍŠILOVÁ a Pavel MUSIL. Determination of Gentiopicroside in Extracts of Centaurium erythreae and Gentiana lutea by Micellar Electrokinetic Capillary Chromatography. J.Liquid Chromatogr. Rel. Technol. New York: Marcel Dekker, 2000, roč. 23, č. 12, s. 1831-1839. ISSN 1082-6076.

@article{326211,
   author = {Glatz, Zdeněk and Pospíšilová, Jiřina and Musil, Pavel},
   article_location = {New York},
   article_number = {12},
   keywords = {micellar electrokinetic chromatography; gentiopicroside; gentiana; centaurium},
   language = {eng},
   issn = {1082-6076},
   journal = {J.Liquid Chromatogr. Rel. Technol.},
   title = {Determination of Gentiopicroside in Extracts of Centaurium erythreae and Gentiana lutea by Micellar Electrokinetic Capillary Chromatography},
   volume = {23},
   year = {2000}
}

TY  - JOUR
ID  - 326211
AU  - Glatz, Zdeněk - Pospíšilová, Jiřina - Musil, Pavel
PY  - 2000
TI  - Determination of Gentiopicroside in Extracts of Centaurium erythreae and Gentiana lutea by Micellar Electrokinetic Capillary Chromatography
JF  - J.Liquid Chromatogr. Rel. Technol.
VL  - 23
IS  - 12
SP  - 1831
EP  - 1831
PB  - Marcel Dekker
SN  - 10826076
KW  - micellar electrokinetic chromatography
KW  - gentiopicroside
KW  - gentiana
KW  - centaurium
N2  - Micellar electrokinetic capillary chromatography has been developed as a promising method for the determination of gentiopicroside. The separation conditions have been optimised with respect to the different parameters including SDS concentration, pH and ion strength of the background electrolyte, and temperature of capillary. A buffer consisting of 100 mM SDS in 20 mM sodium dihydrogen phosphate, 20 mM disodium tetraborate pH 8.6 was found to be the most suitable electrolyte for this separation. The applied voltage of 28 kV (positive polarity) and the temperature of capillary 20 oC gave the best separation of secoiridiods. The linear detection range for concentration versus peak area for the assay is from 5 to 500 mg . ml-1 (correlation coefficient 0.9998) with a detection limit of 0.3 mg . ml-1. The inter-day reproducibility of the peak area was 2.5 % and the inter-day reproducibility of the migration time was below 0.16 %. The developed method was successfully used to quantify gentiopicroside in extracts of the overground parts of Centaurium erythreae and the roots of Gentiana lutea, and in the liqueur Becherovka. The results were in good agreement with those determined by HPLC.
ER  -

GLATZ, Zdeněk; Jiřina POSPÍŠILOVÁ a Pavel MUSIL. Determination of Gentiopicroside in Extracts of Centaurium erythreae and Gentiana lutea by Micellar Electrokinetic Capillary Chromatography. \textit{J.Liquid Chromatogr. Rel. Technol.}. New York: Marcel Dekker, 2000, roč.~23, č.~12, s.~1831-1839. ISSN~1082-6076.

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