Preparation of specific probes detecting prophages of
serogroups A, B and F in lysogenic strains of Staphylococcus
aureus

D 2000

Preparation of specific probes detecting prophages of serogroups A, B and F in lysogenic strains of Staphylococcus aureus

DOŠKAŘ, Jiří; Petra PALLOVÁ; Roman PANTŮČEK; Stanislav ROSYPAL; Vladislava RŮŽIČKOVÁ et al.

Základní údaje

Originální název

Preparation of specific probes detecting prophages of serogroups A, B and F in lysogenic strains of Staphylococcus aureus

Autoři

DOŠKAŘ, Jiří; Petra PALLOVÁ; Roman PANTŮČEK ; Stanislav ROSYPAL; Vladislava RŮŽIČKOVÁ; Pavla PANTŮČKOVÁ a Jana KAILEROVÁ

Vydání

Kolding, 9th International Symposium on Staphylococci and Staphylococcal Infections, s. 102-102, 2000

Nakladatel

JDS

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

Genetika a molekulární biologie

Stát vydavatele

Dánsko

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/00:00002294

Organizační jednotka

Přírodovědecká fakulta

Změněno: 2. 1. 2001 15:55, prof. RNDr. Roman Pantůček, Ph.D.

Anotace

V originále

On the basis of quantitative HindIII-restriction analysis of genomic DNAs, the S. aureus bacteriophages of International Typing Set were divided into five clusters designated as A, F, Ba, Bb and Bc. The clusters A and F include all the phages of serogroups A and F and correspond with the species 3A and 77 proposed by Ackermann and DuBow (1987). On the other hand, the phages of serogroup B were divided into three clusters designated as Ba, Bb and Bc that differ significantly each from the other in their restriction pattern. The clusters Ba and Bb may represent two separate species, while the cluster Bc, formed at a low similarity level, may include more than one phage species. For each of the phage serogroups A, B and F, the common HindIII-restriction fragments were identified of which those of phage 3A (1700 bp), of 53 (4060 bp) and of 77 (8300 bp) were used for the preparation of probes specific to the phages of serogroups A, B and F. These probes have been shown to be very effective, it making possible to determine up to three different prophages in a given lysogenic strain at the same time. Restriction maps of the phages 3A, 53 and 77 representing individual serogroups were constructed. The restriction map of phage 3A and that of phage 77 is linear, whereas that of phage 53 is circular and exhibits a circular permutation. DNAs of the phages of serogroups A and F have cohesive ends. On each restriction map, the sites corresponding to specific probes are indicated. The size of intact genomic DNA of all phages estimated by the PFGE varies within the range 41.5 - 46.2 kb.

Návaznosti

GA301/99/D075, projekt VaV

Název: Genotypová diagnostika a typizace klinicky významných koaguláza negativních stafylokoků

Investor: Grantová agentura ČR, Genotypová diagnostika a typizace klinicky významných koaguláza negativních stafylokoků

MSM 143100008, záměr

Název: Genomy a jejich funkce

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Genomy a jejich funkce

Citovat

DOŠKAŘ, Jiří; Petra PALLOVÁ; Roman PANTŮČEK; Stanislav ROSYPAL; Vladislava RŮŽIČKOVÁ; Pavla PANTŮČKOVÁ a Jana KAILEROVÁ. Preparation of specific probes detecting prophages of serogroups A, B and F in lysogenic strains of Staphylococcus aureus. In 9th International Symposium on Staphylococci and Staphylococcal Infections. Kolding: JDS, 2000, s. 102.

@inproceedings{327171,
   author = {Doškař, Jiří and Pallová, Petra and Pantůček, Roman and Rosypal, Stanislav and Růžičková, Vladislava and Pantůčková, Pavla and Kailerová, Jana},
   address = {Kolding},
   booktitle = {9th International Symposium on Staphylococci and Staphylococcal Infections},
   language = {eng},
   location = {Kolding},
   pages = {102-102},
   publisher = {JDS},
   title = {Preparation of specific probes detecting prophages of serogroups A, B and F in lysogenic strains of Staphylococcus aureus},
   year = {2000}
}

TY  - CONF
ID  - 327171
AU  - Doškař, Jiří - Pallová, Petra - Pantůček, Roman - Rosypal, Stanislav - Růžičková, Vladislava - Pantůčková, Pavla - Kailerová, Jana
PY  - 2000
TI  - Preparation of specific probes detecting prophages of serogroups A, B and F in lysogenic strains of Staphylococcus aureus
PB  - JDS
CY  - Kolding
N2  - On the basis of quantitative HindIII-restriction analysis of genomic DNAs, the S. aureus bacteriophages of International Typing Set were divided into five clusters designated as A, F, Ba, Bb and Bc. The clusters A and F include all the phages of serogroups A and F and correspond with the species 3A and 77 proposed by Ackermann and DuBow (1987). On the other hand, the phages of serogroup B were divided into three clusters designated as Ba, Bb and Bc that differ significantly each from the other in their restriction pattern. The clusters Ba and Bb may represent two separate species, while the cluster Bc, formed at a low similarity level, may include more than one phage species. For each of the phage serogroups A, B and F, the common HindIII-restriction fragments were identified of which those of phage 3A (1700 bp), of 53 (4060 bp) and of 77 (8300 bp) were used for the preparation of probes specific to the phages of serogroups A, B and F. These probes have been shown to be very effective, it making possible to determine up to three different prophages in a given lysogenic strain at the same time. Restriction maps of the phages 3A, 53 and 77 representing individual serogroups were constructed. The restriction map of phage 3A and that of phage 77 is linear, whereas that of phage 53 is circular and exhibits a circular permutation. DNAs of the phages of serogroups A and F have cohesive ends. On each restriction map, the sites corresponding to specific probes are indicated. The size of intact genomic DNA of all phages estimated by the PFGE varies within the range 41.5 - 46.2 kb.
ER  -

DOŠKAŘ, Jiří; Petra PALLOVÁ; Roman PANTŮČEK; Stanislav ROSYPAL; Vladislava RŮŽIČKOVÁ; Pavla PANTŮČKOVÁ a Jana KAILEROVÁ. Preparation of specific probes detecting prophages of serogroups A, B and F in lysogenic strains of Staphylococcus aureus. In \textit{9th International Symposium on Staphylococci and Staphylococcal Infections}. Kolding: JDS, 2000, s.~102.

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