GLATZ, Zdeněk a Hana MAŠLAŇOVÁ. Specific thiol determination by micellar electrokinetic chromatography and on-column detection reaction with 2,2-dipyridyldisulphide. Journal of Chromatography A. Netherlands: Elsevier, 2000, roč. 895, 1-2, s. 179-187. ISSN 0021-9606. |
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@article{329154, author = {Glatz, Zdeněk and Mašlaňová, Hana}, article_location = {Netherlands}, article_number = {1-2}, keywords = {thiol; on-column derivatization; MEKC}, language = {eng}, issn = {0021-9606}, journal = {Journal of Chromatography A}, title = {Specific thiol determination by micellar electrokinetic chromatography and on-column detection reaction with 2,2-dipyridyldisulphide}, volume = {895}, year = {2000} }
TY - JOUR ID - 329154 AU - Glatz, Zdeněk - Mašlaňová, Hana PY - 2000 TI - Specific thiol determination by micellar electrokinetic chromatography and on-column detection reaction with 2,2-dipyridyldisulphide JF - Journal of Chromatography A VL - 895 IS - 1-2 SP - 179 EP - 179 PB - Elsevier SN - 00219606 KW - thiol KW - on-column derivatization KW - MEKC N2 - A new method for specific determination of glutathione using micellar electrokinetic chromatography and on-column reaction with 2,2 -dipyridyldisulphide is described. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively into the capillary as two discrete plugs separated with a short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione, on-column mixing and reaction occur. Glutathione is in this reaction quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amount of the 2-thiopyridone which is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of glutathione is thus estimated indirectly from the result of 2-thiopyridone determination. ER -
GLATZ, Zdeněk a Hana MAŠLAŇOVÁ. Specific thiol determination by micellar electrokinetic chromatography and on-column detection reaction with 2,2-dipyridyldisulphide. \textit{Journal of Chromatography A}. Netherlands: Elsevier, 2000, roč.~895, 1-2, s.~179-187. ISSN~0021-9606.
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