Role of Arachidonic Acid Metabolites in Effects of TNF-alfa on
Proliferation, Viability, Differentiation and Apoptosis of
Human Leukaemic Cell Line HL-60

D 2000

Role of Arachidonic Acid Metabolites in Effects of TNF-alfa on Proliferation, Viability, Differentiation and Apoptosis of Human Leukaemic Cell Line HL-60

ŠTIKA, Jiří; Jan VONDRÁČEK; Jiřina HOFMANOVÁ; Alois KOZUBÍK; Vladimír ŠIMEK et al.

Základní údaje

Originální název

Role of Arachidonic Acid Metabolites in Effects of TNF-alfa on Proliferation, Viability, Differentiation and Apoptosis of Human Leukaemic Cell Line HL-60

Autoři

ŠTIKA, Jiří ; Jan VONDRÁČEK; Jiřina HOFMANOVÁ; Alois KOZUBÍK a Vladimír ŠIMEK

Vydání

First edition. České Budějovice, Cells II, s. 135-135, 2000

Nakladatel

Kopp nakladatelství

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

30102 Immunology

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/00:00003964

Organizační jednotka

Přírodovědecká fakulta

ISBN

80-7232-120-X

Změněno: 8. 1. 2002 15:22, Mgr. Jiří Štika, Ph.D.

Anotace

V originále

Tumor necrosis factor-a (TNF-a) has been found to reduce cellular proliferation and viability, and to induce apoptosis, as well as a partial differentiation. These effects could contribute to elimination of leukemic cells. Using human leukemic cell line HL-60 as a model, we administered cyclooxygenase (indomethacin) and lipoxygenase (MK-886) inhibitors together with TNF-a, in order to evaluate the possible role of prostaglandins and leukotrienes in these processes. The following methods were used to assess cell proliferation, differentiation and apoptosis: cells were counted with haemocytometer; apoptosis and cell cycle were quantified using flowcytometry and fluorescence microscopy; viability was detected by means of light microscopy and flowcytometry; differentiation was followed as nonspecific esterase activity. Our results indicate that indomethacin can potentiate TNF-a-induced apoptosis, resulting in the decrease of number of cells and their viability during simultaneous treatment with TNF-a. However, no effect of indomethacin on the TNF-a-induced differentiation was observed. MK-886 reduced proliferation and viability of HL-60 cells and induced their apoptosis. Similar effects were observed when it was administered in combination with TNF-a. MK-886 strongly potentiated differentiation after TNF-a treatment, but did not induce differentiation when administered alone. In conclusion, inhibition of leukotriene production could potentiate differentiation by TNF-a, while prostaglandins could play a negative role in proapoptotic effects of TNF-a.

Návaznosti

GA524/99/0694, projekt VaV

Název: Vysoce nenasycené mastné kyseliny a cytokiny - jejich úloha pro zachování homeostázy na úrovni buněčných populací

Citovat

ŠTIKA, Jiří; Jan VONDRÁČEK; Jiřina HOFMANOVÁ; Alois KOZUBÍK a Vladimír ŠIMEK. Role of Arachidonic Acid Metabolites in Effects of TNF-alfa on Proliferation, Viability, Differentiation and Apoptosis of Human Leukaemic Cell Line HL-60. In Cells II. First edition. České Budějovice: Kopp nakladatelství, 2000, s. 135. ISBN 80-7232-120-X.

@inproceedings{332331,
   author = {Štika, Jiří and Vondráček, Jan and Hofmanová, Jiřina and Kozubík, Alois and Šimek, Vladimír},
   address = {České Budějovice},
   booktitle = {Cells II},
   edition = {First edition},
   language = {eng},
   location = {České Budějovice},
   isbn = {80-7232-120-X},
   pages = {135-135},
   publisher = {Kopp nakladatelství},
   title = {Role of Arachidonic Acid Metabolites in Effects of TNF-alfa on Proliferation, Viability, Differentiation and Apoptosis of Human Leukaemic Cell Line HL-60},
   year = {2000}
}

TY  - CONF
ID  - 332331
AU  - Štika, Jiří - Vondráček, Jan - Hofmanová, Jiřina - Kozubík, Alois - Šimek, Vladimír
PY  - 2000
TI  - Role of Arachidonic Acid Metabolites in Effects of TNF-alfa on Proliferation, Viability, Differentiation and Apoptosis of Human Leukaemic Cell Line HL-60
PB  - Kopp nakladatelství
CY  - České Budějovice
SN  - 807232120X
N2  - Tumor necrosis factor-a (TNF-a) has been found to reduce cellular proliferation and viability, and to induce apoptosis, as well as a partial differentiation. These effects could contribute to elimination of leukemic cells. Using human leukemic cell line HL-60 as a model, we administered cyclooxygenase (indomethacin) and lipoxygenase (MK-886) inhibitors together with TNF-a, in order to evaluate the possible role of prostaglandins and leukotrienes in these processes. The following methods were used to assess cell proliferation, differentiation and apoptosis: cells were counted with haemocytometer; apoptosis and cell cycle were quantified using flowcytometry and fluorescence microscopy; viability was detected by means of light microscopy and flowcytometry; differentiation was followed as nonspecific esterase activity. Our results indicate that indomethacin can potentiate TNF-a-induced apoptosis, resulting in the decrease of number of cells and their viability during simultaneous treatment with TNF-a. However, no effect of indomethacin on the TNF-a-induced differentiation was observed. MK-886 reduced proliferation and viability of HL-60 cells and induced their apoptosis. Similar effects were observed when it was administered in combination with TNF-a. MK-886 strongly potentiated differentiation after TNF-a treatment, but did not induce differentiation when administered alone. In conclusion, inhibition of leukotriene production could potentiate differentiation by TNF-a, while prostaglandins could play a negative role in proapoptotic effects of TNF-a.
ER  -

ŠTIKA, Jiří; Jan VONDRÁČEK; Jiřina HOFMANOVÁ; Alois KOZUBÍK a Vladimír ŠIMEK. Role of Arachidonic Acid Metabolites in Effects of TNF-alfa on Proliferation, Viability, Differentiation and Apoptosis of Human Leukaemic Cell Line HL-60. In \textit{Cells II}. First edition. České Budějovice: Kopp nakladatelství, 2000, s.~135. ISBN~80-7232-120-X.

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