D 2000

Mycelium extracts of Armillaria trigger a hypersensitive response in sensitive plant cells

KAŠPAROVSKÝ, Tomáš, Lea LOJKOVÁ a Vladimír MIKEŠ

Základní údaje

Originální název

Mycelium extracts of Armillaria trigger a hypersensitive response in sensitive plant cells

Autoři

KAŠPAROVSKÝ, Tomáš (203 Česká republika, garant), Lea LOJKOVÁ a Vladimír MIKEŠ (203 Česká republika)

Vydání

Catania, Book of Abstracts of EFPP Congres 2000, Taormina, Italy, s. 63-63, 2000

Nakladatel

EFPP

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Itálie

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/00:00002368

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Elicitor; Armillaria; defence reactiion
Změněno: 20. 5. 2009 16:43, prof. Mgr. Tomáš Kašparovský, Ph.D.

Anotace

V originále

The genus Armillaria includes serious pathogens causing root rot of a broad host range of plants. Water extracts of mycelium of A. tabescens inhibited the growth of Picea cell cultures and disturbed photosynthesis in leaves of Acer pseudoplatanus measured by fluorimetry. Extracts of mycelium of A. tabescens obtained by supercritical fluid extraction (SFE) trigger a hypersensitive response in suspension of tobacco cells accompanied by an alkalization of extracellular medium and production of active oxygen species. The HPLC analysis and GC-MS of SFE proved the presence of several toxins and sterols, namely ergosterol and dehydroergosterol. This reponse was calcium dependent and was stopped by inhibitors of protein kinases. At least two sterols had elicitor activity; one of them was ergosterol.

Česky

The genus Armillaria includes serious pathogens causing root rot of a broad host range of plants. Water extracts of mycelium of A. tabescens inhibited the growth of Picea cell cultures and disturbed photosynthesis in leaves of Acer pseudoplatanus measured by fluorimetry. Extracts of mycelium of A. tabescens obtained by supercritical fluid extraction (SFE) trigger a hypersensitive response in suspension of tobacco cells accompanied by an alkalization of extracellular medium and production of active oxygen species. The HPLC analysis and GC-MS of SFE proved the presence of several toxins and sterols, namely ergosterol and dehydroergosterol. This reponse was calcium dependent and was stopped by inhibitors of protein kinases. At least two sterols had elicitor activity; one of them was ergosterol.

Návaznosti

MSM 143100005, záměr
Název: Strukturně-funkční vztahy biomolekul a jejich role v metabolismu
Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Strukturně-funkční vztahy biomolekul a jejich role v metabolismu