| HOFR, Ctirad a Viktor BRABEC. Thermal and thermodynamic properties of duplex DNA containing site-specific interstrand cross-link of antitumor cisplatin or its clinically ineffective trans isomer. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2001, roč. 276, č. 276, s. 9655-9661. ISSN 0021-9258. |
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@article{344040,
author = {Hofr, Ctirad and Brabec, Viktor},
article_location = {Bethesda, USA},
article_number = {276},
language = {eng},
issn = {0021-9258},
journal = {Journal of Biological Chemistry},
title = {Thermal and thermodynamic properties of duplex DNA containing site-specific interstrand cross-link of antitumor cisplatin or its clinically ineffective trans isomer},
volume = {276},
year = {2001}
}
TY - JOUR
ID - 344040
AU - Hofr, Ctirad - Brabec, Viktor
PY - 2001
TI - Thermal and thermodynamic properties of duplex DNA containing site-specific interstrand cross-link of antitumor cisplatin or its clinically ineffective trans isomer
JF - Journal of Biological Chemistry
VL - 276
IS - 276
SP - 9655
EP - 9655
PB - Amer. Soc. Biochem. Mol. Biol.
SN - 00219258
N2 - The effect of the site-specific interstrand cross-link formed by cisplatin or transplatin on the thermal stability and energetics of 20-base pairs DNA duplex is reported. The cross-linked or unplatinated 20-bp duplexes were investigated with the aid of differential scanning calorimetry, temperature-dependent UV absorption and circular dichroism. The cross-link of both platinum isomers increases the thermal stability of the modified duplexes by changing the molecularity of denaturation. The structural perturbation due to the interstrand cross-link of cisplatin increases entropy of the duplex. By contrast, the interstrand cross-link of transplatin is enthalpically almost neutral. These differences are consistent with distinct conformational distortions induced by the interstrand cross-links of the two isomers. It has been proposed that the results of the present work might be associated with distinctly different antitumor effects of these platinum compounds.
ER -
HOFR, Ctirad a Viktor BRABEC. Thermal and thermodynamic properties of duplex DNA containing site-specific interstrand cross-link of antitumor cisplatin or its clinically ineffective trans isomer. \textit{Journal of Biological Chemistry}. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2001, roč.~276, č.~276, s.~9655-9661. ISSN~0021-9258.
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