Thermal and thermodynamic properties of duplex DNA containing
site-specific interstrand cross-link of antitumor cisplatin or
its clinically ineffective trans isomer

J 2001

Thermal and thermodynamic properties of duplex DNA containing site-specific interstrand cross-link of antitumor cisplatin or its clinically ineffective trans isomer

HOFR, Ctirad a Viktor BRABEC

Základní údaje

Originální název

Thermal and thermodynamic properties of duplex DNA containing site-specific interstrand cross-link of antitumor cisplatin or its clinically ineffective trans isomer

Autoři

HOFR, Ctirad a Viktor BRABEC

Vydání

Journal of Biological Chemistry, Bethesda, USA, Amer. Soc. Biochem. Mol. Biol. 2001, 0021-9258

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10610 Biophysics

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 7.258

Kód RIV

RIV/00216224:14310/01:00003419

Organizační jednotka

Přírodovědecká fakulta

Změněno: 25. 5. 2001 15:20, prof. RNDr. Viktor Brabec, DrSc.

Anotace

V originále

The effect of the site-specific interstrand cross-link formed by cisplatin or transplatin on the thermal stability and energetics of 20-base pairs DNA duplex is reported. The cross-linked or unplatinated 20-bp duplexes were investigated with the aid of differential scanning calorimetry, temperature-dependent UV absorption and circular dichroism. The cross-link of both platinum isomers increases the thermal stability of the modified duplexes by changing the molecularity of denaturation. The structural perturbation due to the interstrand cross-link of cisplatin increases entropy of the duplex. By contrast, the interstrand cross-link of transplatin is enthalpically almost neutral. These differences are consistent with distinct conformational distortions induced by the interstrand cross-links of the two isomers. It has been proposed that the results of the present work might be associated with distinctly different antitumor effects of these platinum compounds.

Návaznosti

GA305/99/0695, projekt VaV

Název: Ovlivnění konformace DNA protinádorově účinnými komplexy kovů. Vztah k vývoji nových cytostatik.

Citovat

HOFR, Ctirad a Viktor BRABEC. Thermal and thermodynamic properties of duplex DNA containing site-specific interstrand cross-link of antitumor cisplatin or its clinically ineffective trans isomer. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2001, roč. 276, č. 276, s. 9655-9661. ISSN 0021-9258.

@article{344040,
   author = {Hofr, Ctirad and Brabec, Viktor},
   article_location = {Bethesda, USA},
   article_number = {276},
   language = {eng},
   issn = {0021-9258},
   journal = {Journal of Biological Chemistry},
   title = {Thermal and thermodynamic properties of duplex DNA containing site-specific interstrand cross-link of antitumor cisplatin or its clinically ineffective trans isomer},
   volume = {276},
   year = {2001}
}

TY  - JOUR
ID  - 344040
AU  - Hofr, Ctirad - Brabec, Viktor
PY  - 2001
TI  - Thermal and thermodynamic properties of duplex DNA containing site-specific interstrand cross-link of antitumor cisplatin or its clinically ineffective trans isomer
JF  - Journal of Biological Chemistry
VL  - 276
IS  - 276
SP  - 9655
EP  - 9655
PB  - Amer. Soc. Biochem. Mol. Biol.
SN  - 00219258
N2  - The effect of the site-specific interstrand cross-link formed by cisplatin or transplatin on the thermal stability and energetics of 20-base pairs DNA duplex is reported. The cross-linked or unplatinated 20-bp duplexes were investigated with the aid of differential scanning calorimetry, temperature-dependent UV absorption and circular dichroism. The cross-link of both platinum isomers increases the thermal stability of the modified duplexes by changing the molecularity of denaturation. The structural perturbation due to the interstrand cross-link of cisplatin increases entropy of the duplex. By contrast, the interstrand cross-link of transplatin is enthalpically almost neutral. These differences are consistent with distinct conformational distortions induced by the interstrand cross-links of the two isomers. It has been proposed that the results of the present work might be associated with distinctly different antitumor effects of these platinum compounds.
ER  -

HOFR, Ctirad a Viktor BRABEC. Thermal and thermodynamic properties of duplex DNA containing site-specific interstrand cross-link of antitumor cisplatin or its clinically ineffective trans isomer. \textit{Journal of Biological Chemistry}. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2001, roč.~276, č.~276, s.~9655-9661. ISSN~0021-9258.

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