Cytoskeleton and mitochondrial topology in Xantophyllomyces
dendrorhous (Phaffia rhodozyma)

J 2000

Cytoskeleton and mitochondrial topology in Xantophyllomyces dendrorhous (Phaffia rhodozyma)

SLANINOVÁ, Iva, Judit KUCSERA a Augustin SVOBODA

Základní údaje

Originální název

Cytoskeleton and mitochondrial topology in Xantophyllomyces dendrorhous (Phaffia rhodozyma)

Autoři

SLANINOVÁ, Iva, Judit KUCSERA a Augustin SVOBODA

Vydání

Folia Microbiologica, Praha, MÚ ČAV, 2000, 0015-5632

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.752

Kód RIV

RIV/00216224:14110/00:00003447

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

cytoskeleton; Xanthophyllomyces; Phaffia rhodozyma; microtubules; mitochondria

Štítky

Cytoskeleton, Microtubules, mitochondria, Phaffia rhodozyma, Xanthophyllomyces

Změněno: 20. 12. 2000 08:21, prof. MUDr. Iva Slaninová, Ph.D.

Anotace

V originále

The morphology of budding and conjugating cells and associated changes in microtubules and actin distribution were studied in the yeast Phaffia rhodozyma (Basidiomycetes) by phase-contrast and fluorescence microscopy. The non-budding interphase cell showed a nucleus situated in the central position and bundles of cytoplasmic microtubules either stretching parallel to the longitudinal cell axis or randomly distributed in the cell; none of these, however, were in contact with the nucleus. During mitosis, the nucleus divided in the daughter cell, cytoplasmic microtubules disappeared and were replaced by a spindle. The cytoplasmic microtubules reappeared after mitosis had finished. Actin patches were present in both the bud and the mother cell. Cells were induced to mate by nitrogen depletion. The partner cells fused at their conjugation projections where actin patches had been accumulated . Cell fusion resulted in a zygote that produced basidiophore with parallel bundles of microtubules extended along the basidiophore axis and with actin patches concentrated at the cell poles. The fused nucleus moved towards the basidiophore tip. During this movement, meiotic division was taking place; the nuclei were eventually distributed to basidiospores. Mitochondria visualised by means of DIOC6 (3) appeared as vesicles of various sizes; their large amounts were found, often lying adjacent to microtubules, in the cytoplasm of both vegetative cells and zygotes.

Návaznosti

GA204/00/0394, projekt VaV

Název: Buněčná stěna kvasinek jako extracelulární matrix: cytoskelet a stěnové funkce

Investor: Grantová agentura ČR, Buněčná stěna kvasinek jako extracelulární matrix: cytoskelet a stěnové funkce

GA204/99/D025, projekt VaV

Název: Úloha cytoskeletu a buněčné stěny v polarizaci buňky

Investor: Grantová agentura ČR, Úloha cytoskeletu a buněčné stěny v polarizaci buňky

Citovat

SLANINOVÁ, Iva, Judit KUCSERA a Augustin SVOBODA. Cytoskeleton and mitochondrial topology in Xantophyllomyces dendrorhous (Phaffia rhodozyma). Folia Microbiologica. Praha: MÚ ČAV, 2000, roč. 45, č. 1, s. 89. ISSN 0015-5632.

@article{344791,
   author = {Slaninová, Iva and Kucsera, Judit and Svoboda, Augustin},
   article_location = {Praha},
   article_number = {1},
   keywords = {cytoskeleton; Xanthophyllomyces; Phaffia rhodozyma; microtubules; mitochondria},
   language = {eng},
   issn = {0015-5632},
   journal = {Folia Microbiologica},
   title = {Cytoskeleton and mitochondrial topology in Xantophyllomyces dendrorhous (Phaffia rhodozyma)},
   volume = {45},
   year = {2000}
}

TY  - JOUR
ID  - 344791
AU  - Slaninová, Iva - Kucsera, Judit - Svoboda, Augustin
PY  - 2000
TI  - Cytoskeleton and mitochondrial topology in Xantophyllomyces dendrorhous (Phaffia rhodozyma)
JF  - Folia Microbiologica
VL  - 45
IS  - 1
SP  - 89
EP  - 89
PB  - MÚ ČAV
SN  - 00155632
KW  - cytoskeleton
KW  - Xanthophyllomyces
KW  - Phaffia rhodozyma
KW  - microtubules
KW  - mitochondria
N2  - The morphology of budding and conjugating cells and associated changes in microtubules and actin distribution were studied in the yeast Phaffia rhodozyma (Basidiomycetes) by phase-contrast and fluorescence microscopy. The non-budding interphase cell showed a nucleus situated in the central position and bundles of cytoplasmic microtubules either stretching parallel to the longitudinal cell axis or randomly distributed in the cell; none of these, however, were in contact with the nucleus. During mitosis, the nucleus divided in the daughter cell, cytoplasmic microtubules disappeared and were replaced by a spindle. The cytoplasmic microtubules reappeared after mitosis had finished. Actin patches were present in both the bud and the mother cell. Cells were induced to mate by nitrogen depletion. The partner cells fused at their conjugation projections where actin patches had been accumulated . Cell fusion resulted in a zygote that produced basidiophore with parallel bundles of microtubules extended along the basidiophore axis and with actin patches concentrated at the cell poles. The fused nucleus moved towards the basidiophore tip. During this movement, meiotic division was taking place; the nuclei were eventually distributed to basidiospores. Mitochondria visualised by means of DIOC6 (3) appeared as vesicles of various sizes; their large amounts were found, often lying adjacent to microtubules, in the cytoplasm of both vegetative cells and zygotes.
ER  -

SLANINOVÁ, Iva, Judit KUCSERA a Augustin SVOBODA. Cytoskeleton and mitochondrial topology in Xantophyllomyces dendrorhous (Phaffia rhodozyma). \textit{Folia Microbiologica}. Praha: MÚ ČAV, 2000, roč.~45, č.~1, s.~89. ISSN~0015-5632.

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