2000
Vliv alkaloidů Chelidonium majus na nádorové buňky a kvasinky
SLANINOVÁ, Iva, Eva TÁBORSKÁ, Hana BOCHOŘÁKOVÁ a Jiří SLANINAZákladní údaje
Originální název
Vliv alkaloidů Chelidonium majus na nádorové buňky a kvasinky
Název anglicky
The effect of Chelidonius majus alkaloids on tumor and yeast cells
Autoři
Vydání
Cěské Budějovice, Cells II, s. 97-97, Cells II, 2000
Nakladatel
KOPP Publishing
Další údaje
Typ výsledku
Stať ve sborníku
Obor
10600 1.6 Biological sciences
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14110/00:00003448
Organizační jednotka
Lékařská fakulta
Klíčová slova anglicky
benzophenenthridine alkaloids; cytotoxicity; cytoskeleton;
Změněno: 20. 12. 2000 08:45, prof. MUDr. Iva Slaninová, Ph.D.
V originále
Four quarternary benzo(c)phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine and two quaternary protoberberine alkaloids berberine and koptisine, were examined for their effects on HeLa cell line (human cervix carcinoma cells) and yeasts S. cerevisiae and Sch. japonicus var. versatilis. The agent concentrations ranged from 1 to 100 mg/ml. Cytotoxicity by neutral red (NR) uptake assay using 96-well plates was tested. In vivo antimicrotubular effect by indirect immunofluorescence of tubulin was tested. ID50 was assessed: sanguinarine ID50 = 0,8 mg/ml, sanguilutine ID50 = 8,3 mg/ml, chelerythrine ID50 = 6,2 mg/ml , chelilutine ID50 = 5,2 mg/ml, coptisine ID50 = 2,6 mg/ml and berberine ID50 =10,0 mg/ml. Alkaloids at lower concentrations (5 to 10 mg/ml) produced a decrease in microtubule numbers, particularly at the cell periphery. The strongest effect was observed in sanguinarine which showed microtubule rearangement at the concentration 0,1 mg/ml and toxic effect at the concentration 1 mg/ml. In yeasts, neither microtubules nor cell growth were affected to any noticable extent even at the highest concentration (100 mg/ml) of the alkaloids.The dynamics of alkaloid distribution in cells was recorded by viewing fluorescence of alkaloids through filters with the excitation spectrum of 355 to 425 nm and 450 to 490 nm. In HeLa cells sanguinarine penetrated into nuclei few minutes after treatment while sanguilutine produced crystal-shaped bodies accumulated in the area of Golgi apparatus. Chelerythrine and chelilutine showed fluorescence in areas near the nucleus. Koptisine and berberine showed 3 type of fluorescence. Fluorescence of some lattice in cytoplasm, fluorescence of small vesicles and nucleoli fluorescence.
Anglicky
Four quarternary benzo(c)phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine and two quaternary protoberberine alkaloids berberine and koptisine, were examined for their effects on HeLa cell line (human cervix carcinoma cells) and yeasts S. cerevisiae and Sch. japonicus var. versatilis. The agent concentrations ranged from 1 to 100 mg/ml. Cytotoxicity by neutral red (NR) uptake assay using 96-well plates was tested. In vivo antimicrotubular effect by indirect immunofluorescence of tubulin was tested. ID50 was assessed: sanguinarine ID50 = 0,8 mg/ml, sanguilutine ID50 = 8,3 mg/ml, chelerythrine ID50 = 6,2 mg/ml , chelilutine ID50 = 5,2 mg/ml, coptisine ID50 = 2,6 mg/ml and berberine ID50 =10,0 mg/ml. Alkaloids at lower concentrations (5 to 10 mg/ml) produced a decrease in microtubule numbers, particularly at the cell periphery. The strongest effect was observed in sanguinarine which showed microtubule rearangement at the concentration 0,1 mg/ml and toxic effect at the concentration 1 mg/ml. In yeasts, neither microtubules nor cell growth were affected to any noticable extent even at the highest concentration (100 mg/ml) of the alkaloids.The dynamics of alkaloid distribution in cells was recorded by viewing fluorescence of alkaloids through filters with the excitation spectrum of 355 to 425 nm and 450 to 490 nm. In HeLa cells sanguinarine penetrated into nuclei few minutes after treatment while sanguilutine produced crystal-shaped bodies accumulated in the area of Golgi apparatus. Chelerythrine and chelilutine showed fluorescence in areas near the nucleus. Koptisine and berberine showed 3 type of fluorescence. Fluorescence of some lattice in cytoplasm, fluorescence of small vesicles and nucleoli fluorescence.
Návaznosti
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