SLANINOVÁ, Iva, Eva TÁBORSKÁ, Hana BOCHOŘÁKOVÁ and Jiří SLANINA. Vliv alkaloidů Chelidonium majus na nádorové buňky a kvasinky (The effect of Chelidonius majus alkaloids on tumor and yeast cells). In Cells II. Cěské Budějovice: KOPP Publishing. p. 97. Cells II. 2000.
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Basic information
Original name Vliv alkaloidů Chelidonium majus na nádorové buňky a kvasinky
Name (in English) The effect of Chelidonius majus alkaloids on tumor and yeast cells
Authors SLANINOVÁ, Iva, Eva TÁBORSKÁ, Hana BOCHOŘÁKOVÁ and Jiří SLANINA.
Edition Cěské Budějovice, Cells II, p. 97-97, Cells II, 2000.
Publisher KOPP Publishing
Other information
Type of outcome Proceedings paper
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14110/00:00003448
Organization unit Faculty of Medicine
Keywords in English benzophenenthridine alkaloids; cytotoxicity; cytoskeleton;
Tags benzophenenthridine alkaloids, Cytoskeleton, cytotoxicity
Changed by Changed by: prof. MUDr. Iva Slaninová, Ph.D., učo 2105. Changed: 20/12/2000 08:45.
Abstract
Four quarternary benzo(c)phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine and two quaternary protoberberine alkaloids berberine and koptisine, were examined for their effects on HeLa cell line (human cervix carcinoma cells) and yeasts S. cerevisiae and Sch. japonicus var. versatilis. The agent concentrations ranged from 1 to 100 mg/ml. Cytotoxicity by neutral red (NR) uptake assay using 96-well plates was tested. In vivo antimicrotubular effect by indirect immunofluorescence of tubulin was tested. ID50 was assessed: sanguinarine ID50 = 0,8 mg/ml, sanguilutine ID50 = 8,3 mg/ml, chelerythrine ID50 = 6,2 mg/ml , chelilutine ID50 = 5,2 mg/ml, coptisine ID50 = 2,6 mg/ml and berberine ID50 =10,0 mg/ml. Alkaloids at lower concentrations (5 to 10 mg/ml) produced a decrease in microtubule numbers, particularly at the cell periphery. The strongest effect was observed in sanguinarine which showed microtubule rearangement at the concentration 0,1 mg/ml and toxic effect at the concentration 1 mg/ml. In yeasts, neither microtubules nor cell growth were affected to any noticable extent even at the highest concentration (100 mg/ml) of the alkaloids.The dynamics of alkaloid distribution in cells was recorded by viewing fluorescence of alkaloids through filters with the excitation spectrum of 355 to 425 nm and 450 to 490 nm. In HeLa cells sanguinarine penetrated into nuclei few minutes after treatment while sanguilutine produced crystal-shaped bodies accumulated in the area of Golgi apparatus. Chelerythrine and chelilutine showed fluorescence in areas near the nucleus. Koptisine and berberine showed 3 type of fluorescence. Fluorescence of some lattice in cytoplasm, fluorescence of small vesicles and nucleoli fluorescence.
Abstract (in English)
Four quarternary benzo(c)phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine and two quaternary protoberberine alkaloids berberine and koptisine, were examined for their effects on HeLa cell line (human cervix carcinoma cells) and yeasts S. cerevisiae and Sch. japonicus var. versatilis. The agent concentrations ranged from 1 to 100 mg/ml. Cytotoxicity by neutral red (NR) uptake assay using 96-well plates was tested. In vivo antimicrotubular effect by indirect immunofluorescence of tubulin was tested. ID50 was assessed: sanguinarine ID50 = 0,8 mg/ml, sanguilutine ID50 = 8,3 mg/ml, chelerythrine ID50 = 6,2 mg/ml , chelilutine ID50 = 5,2 mg/ml, coptisine ID50 = 2,6 mg/ml and berberine ID50 =10,0 mg/ml. Alkaloids at lower concentrations (5 to 10 mg/ml) produced a decrease in microtubule numbers, particularly at the cell periphery. The strongest effect was observed in sanguinarine which showed microtubule rearangement at the concentration 0,1 mg/ml and toxic effect at the concentration 1 mg/ml. In yeasts, neither microtubules nor cell growth were affected to any noticable extent even at the highest concentration (100 mg/ml) of the alkaloids.The dynamics of alkaloid distribution in cells was recorded by viewing fluorescence of alkaloids through filters with the excitation spectrum of 355 to 425 nm and 450 to 490 nm. In HeLa cells sanguinarine penetrated into nuclei few minutes after treatment while sanguilutine produced crystal-shaped bodies accumulated in the area of Golgi apparatus. Chelerythrine and chelilutine showed fluorescence in areas near the nucleus. Koptisine and berberine showed 3 type of fluorescence. Fluorescence of some lattice in cytoplasm, fluorescence of small vesicles and nucleoli fluorescence.
Links
GA204/99/D025, research and development projectName: Úloha cytoskeletu a buněčné stěny v polarizaci buňky
Investor: Czech Science Foundation, The role of cytoskeleton and cell wall in the cell polarization
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