Vliv alkaloidů Chelidonium majus na nádorové buňky a kvasinky

SLANINOVÁ, Iva, Eva TÁBORSKÁ, Hana BOCHOŘÁKOVÁ a Jiří SLANINA. Vliv alkaloidů Chelidonium majus na nádorové buňky a kvasinky. In Cells II. Cěské Budějovice: KOPP Publishing. s. 97. Cells II. 2000.

Základní údaje

• Originální název: Vliv alkaloidů Chelidonium majus na nádorové buňky a kvasinky
• Název anglicky: The effect of Chelidonius majus alkaloids on tumor and yeast cells
• Autoři: SLANINOVÁ, Iva, Eva TÁBORSKÁ, Hana BOCHOŘÁKOVÁ a Jiří SLANINA.
• Vydání: Cěské Budějovice, Cells II, s. 97-97, Cells II, 2000.
• Nakladatel: KOPP Publishing

Další údaje

• Typ výsledku: Stať ve sborníku
• Obor: 10600 1.6 Biological sciences
• Stát vydavatele: Česká republika
• Utajení: není předmětem státního či obchodního tajemství
• Kód RIV: RIV/00216224:14110/00:00003448
• Organizační jednotka: Lékařská fakulta
• Klíčová slova anglicky: benzophenenthridine alkaloids; cytotoxicity; cytoskeleton
• Štítky: benzophenenthridine alkaloids, Cytoskeleton, cytotoxicity

Anotace

Four quarternary benzo(c)phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine and two quaternary protoberberine alkaloids berberine and koptisine, were examined for their effects on HeLa cell line (human cervix carcinoma cells) and yeasts S. cerevisiae and Sch. japonicus var. versatilis. The agent concentrations ranged from 1 to 100 mg/ml. Cytotoxicity by neutral red (NR) uptake assay using 96-well plates was tested. In vivo antimicrotubular effect by indirect immunofluorescence of tubulin was tested. ID50 was assessed: sanguinarine ID50 = 0,8 mg/ml, sanguilutine ID50 = 8,3 mg/ml, chelerythrine ID50 = 6,2 mg/ml , chelilutine ID50 = 5,2 mg/ml, coptisine ID50 = 2,6 mg/ml and berberine ID50 =10,0 mg/ml. Alkaloids at lower concentrations (5 to 10 mg/ml) produced a decrease in microtubule numbers, particularly at the cell periphery. The strongest effect was observed in sanguinarine which showed microtubule rearangement at the concentration 0,1 mg/ml and toxic effect at the concentration 1 mg/ml. In yeasts, neither microtubules nor cell growth were affected to any noticable extent even at the highest concentration (100 mg/ml) of the alkaloids.The dynamics of alkaloid distribution in cells was recorded by viewing fluorescence of alkaloids through filters with the excitation spectrum of 355 to 425 nm and 450 to 490 nm. In HeLa cells sanguinarine penetrated into nuclei few minutes after treatment while sanguilutine produced crystal-shaped bodies accumulated in the area of Golgi apparatus. Chelerythrine and chelilutine showed fluorescence in areas near the nucleus. Koptisine and berberine showed 3 type of fluorescence. Fluorescence of some lattice in cytoplasm, fluorescence of small vesicles and nucleoli fluorescence.

Anotace anglicky

Four quarternary benzo(c)phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine and two quaternary protoberberine alkaloids berberine and koptisine, were examined for their effects on HeLa cell line (human cervix carcinoma cells) and yeasts S. cerevisiae and Sch. japonicus var. versatilis. The agent concentrations ranged from 1 to 100 mg/ml. Cytotoxicity by neutral red (NR) uptake assay using 96-well plates was tested. In vivo antimicrotubular effect by indirect immunofluorescence of tubulin was tested. ID50 was assessed: sanguinarine ID50 = 0,8 mg/ml, sanguilutine ID50 = 8,3 mg/ml, chelerythrine ID50 = 6,2 mg/ml , chelilutine ID50 = 5,2 mg/ml, coptisine ID50 = 2,6 mg/ml and berberine ID50 =10,0 mg/ml. Alkaloids at lower concentrations (5 to 10 mg/ml) produced a decrease in microtubule numbers, particularly at the cell periphery. The strongest effect was observed in sanguinarine which showed microtubule rearangement at the concentration 0,1 mg/ml and toxic effect at the concentration 1 mg/ml. In yeasts, neither microtubules nor cell growth were affected to any noticable extent even at the highest concentration (100 mg/ml) of the alkaloids.The dynamics of alkaloid distribution in cells was recorded by viewing fluorescence of alkaloids through filters with the excitation spectrum of 355 to 425 nm and 450 to 490 nm. In HeLa cells sanguinarine penetrated into nuclei few minutes after treatment while sanguilutine produced crystal-shaped bodies accumulated in the area of Golgi apparatus. Chelerythrine and chelilutine showed fluorescence in areas near the nucleus. Koptisine and berberine showed 3 type of fluorescence. Fluorescence of some lattice in cytoplasm, fluorescence of small vesicles and nucleoli fluorescence.

Návaznosti

• GA204/99/D025, projekt VaV: Název: Úloha cytoskeletu a buněčné stěny v polarizaci buňky

Investor: Grantová agentura ČR, Úloha cytoskeletu a buněčné stěny v polarizaci buňky