Migrating Schwann cells support the endoneurium of
freeze-treated nerve segment by laminins, fibronectin,
thrombospondin, and chondroitin sulfate.

D 2000

Migrating Schwann cells support the endoneurium of freeze-treated nerve segment by laminins, fibronectin, thrombospondin, and chondroitin sulfate.

DUBOVÝ, Petr; Ivana SVÍŽENSKÁ a Ilona KLUSÁKOVÁ

Základní údaje

Originální název

Migrating Schwann cells support the endoneurium of freeze-treated nerve segment by laminins, fibronectin, thrombospondin, and chondroitin sulfate.

Autoři

DUBOVÝ, Petr; Ivana SVÍŽENSKÁ a Ilona KLUSÁKOVÁ

Vydání

Praha, Sborník lékařský, s. 4-4, Vol. 101, No. 4, 2000

Nakladatel

Karlova Univerzita

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/00:00002596

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Schwann cell; extracellular matrix; peripheral nerve

Štítky

extracellular matrix, Peripheral nerve, Schwann cell

Změněno: 21. 12. 2000 12:15, MUDr. Ivana Hradilová Svíženská, CSc.

Anotace

V originále

The acellular nerve segment is thought to be a method of choice for reparation of nerve defect. Repeated periods of freezing and thawing are frequently used for preparation of the standard acellular nerve segment in experimental application. It was believed that the basal lamina tubes and endoneurial extracellular matrix molecules survive the procedures and promote the growth of regenerating axons. We used a model of the freeze-treated nerve segment to study the molecular content of the endoneurium in absence and presence of Schwann cells. The sciatic nerves of 10 adult female rats were cut and the 10 mm long nerve segments were frozen 5 times with forceps pre-cooled in liquid nitrogen. The rats were secrificed after 14 days. Serial longitudinal frozen sections, 10 ľm thick, were incubated with antibodies against S-100 protein, laminin-1, -2, and -3, fibronectin, thrombospondin, as well as chondroitin sulfate. The binding of primary antibodies was detected with indirect biotin-streptavidin-TRITC immunohistochemical method. Our results suggest that Schwann cells are crucial for axon-promoting condition in the endoneurium of the acellular nerve segment.

Návaznosti

GA309/00/0407, projekt VaV

Název: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Investor: Grantová agentura ČR, Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

GA309/97/0107, projekt VaV

Název: Exprese integrinů při diferenciaci Schwannových buněk a odvozených struktur v periferní nervové soustavě laboratorního potkana

Investor: Grantová agentura ČR, Exprese integrinů při diferenciaci Schwannových buněk a odvozených struktur v periferní nervové soustavě laboratorního potkana

Citovat

DUBOVÝ, Petr; Ivana SVÍŽENSKÁ a Ilona KLUSÁKOVÁ. Migrating Schwann cells support the endoneurium of freeze-treated nerve segment by laminins, fibronectin, thrombospondin, and chondroitin sulfate. In Sborník lékařský. Praha: Karlova Univerzita, 2000, s. 4. Vol. 101, No. 4.

@inproceedings{344836,
   author = {Dubový, Petr and Svíženská, Ivana and Klusáková, Ilona},
   address = {Praha},
   booktitle = {Sborník lékařský},
   keywords = {Schwann cell; extracellular matrix; peripheral nerve},
   language = {eng},
   location = {Praha},
   pages = {4-4},
   publisher = {Karlova Univerzita},
   title = {Migrating Schwann cells support the endoneurium of freeze-treated nerve segment by laminins, fibronectin, thrombospondin, and chondroitin sulfate.},
   year = {2000}
}

TY  - CONF
ID  - 344836
AU  - Dubový, Petr - Svíženská, Ivana - Klusáková, Ilona
PY  - 2000
TI  - Migrating Schwann cells support the endoneurium of freeze-treated nerve segment by laminins, fibronectin, thrombospondin, and chondroitin sulfate.
VL  - Vol. 101, No. 4
PB  - Karlova Univerzita
CY  - Praha
KW  - Schwann cell
KW  - extracellular matrix
KW  - peripheral nerve
N2  - The acellular nerve segment is thought to be a method of choice for reparation of nerve defect. Repeated periods of freezing and thawing are frequently used for preparation of the standard acellular nerve segment in experimental application. It was believed that the basal lamina tubes and endoneurial extracellular matrix molecules survive the procedures and promote the growth of regenerating axons. We used a model of the freeze-treated nerve segment to study the molecular content of the endoneurium in absence and presence of Schwann cells. The sciatic nerves of 10 adult female rats were cut and the 10 mm long nerve segments were frozen 5 times with forceps pre-cooled in liquid nitrogen. The rats were secrificed after 14 days. Serial longitudinal frozen sections, 10 ľm thick, were incubated with antibodies against S-100 protein, laminin-1, -2, and -3, fibronectin, thrombospondin, as well as chondroitin sulfate. The binding of primary antibodies was detected with indirect biotin-streptavidin-TRITC immunohistochemical method. Our results suggest that Schwann cells are crucial for axon-promoting condition in the endoneurium of the acellular nerve segment.
ER  -

DUBOVÝ, Petr; Ivana SVÍŽENSKÁ a Ilona KLUSÁKOVÁ. Migrating Schwann cells support the endoneurium of freeze-treated nerve segment by laminins, fibronectin, thrombospondin, and chondroitin sulfate. In \textit{Sborník lékařský}. Praha: Karlova Univerzita, 2000, s.~4. Vol. 101, No. 4.

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